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Preparation method and micromolecule detection application of metal organic framework material

A metal-organic framework and organic framework technology, which is applied in the direction of material analysis, analysis materials, and measurement devices through electromagnetic means, can solve the problems of low concentration and interference of biomarkers, and achieve low preparation cost, high-efficiency analysis, and large sample volume. low effect

Pending Publication Date: 2021-10-08
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the low concentration of early cancer biomarkers and the interference of other biomolecules in serum, their detection has brought great challenges

Method used

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  • Preparation method and micromolecule detection application of metal organic framework material
  • Preparation method and micromolecule detection application of metal organic framework material
  • Preparation method and micromolecule detection application of metal organic framework material

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Experimental program
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Embodiment 1

[0040] Step 1: Preparation of instruments and reagents: set the matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection mode to positive ion reflection mode;

[0041] Step 2: preparing metal-organic framework series substrates, including the following steps;

[0042] Step 2.1: Mix dimethylformamide, ethanol, deionized water, triethylamine, and terephthalic acid into a closed container, and add gold ferrous chloride or cobalt chloride or nickel chloride respectively;

[0043] Step 2.2: The mixture in step 2.1 was subjected to ultrasonic reaction in a water bath for 4 hours at 0°C;

[0044] Step 2.3: centrifuge the reactant in step 2.2, and wash with deionized water and isopropanol five times each to remove residual dimethylformamide;

[0045] Step 2.4: Disperse the product in step 2.3 in deionized water, and use low-speed centrifugation to separate the ultra-thin metal organic framework material (MOF-UL). According to the difference of metal chlo...

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Abstract

The invention discloses a preparation method and micromolecule detection application of a metal organic framework material, and relates to the field of metal organic framework materials. The preparation method comprises the steps that: dimethylformamide, ethyl alcohol, deionized water, triethylamine and terephthalic acid are mixed and added into a closed container, and metal chloride is added, so that a mixture can be formed; centrifuging and washing are performed after water bath ultrasonic treatment; then dispersing is performed in deionized water, and low-speed centrifugal separation is performed to obtain the ultrathin metal organic framework material; and the ultrathin metal organic framework material is applied to small molecule detection. According to the framework material and the method, the metal ions and the thickness in the metal organic framework material can be accurately adjusted, the defects of a traditional matrix are overcome, the sample size is low, and serum small molecules can be rapidly detected with high throughput and high sensitivity.

Description

technical field [0001] The invention relates to the field of metal organic framework materials, in particular to a preparation method of metal organic framework materials and its small molecule detection application. Background technique [0002] Serum diagnosis of biomarkers is the key to early diagnosis of diseases. Macromolecular substances such as nucleic acids, proteins, etc., can be extended to detectable levels through polymerase chain reaction, fluorescence enhancement and other technical support, so as to be used for disease diagnosis. Compared with macromolecular substances, signal amplification for small molecule metabolites still faces great challenges, and detection methods for small molecule metabolites need to be developed. [0003] At present, the detection of small molecule metabolites mainly relies on two technologies: mass spectrometry and nuclear magnetic resonance. Mass spectrometry remains the primary technique for the detection of small molecule meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 钱昆杨静
Owner SHANGHAI JIAO TONG UNIV
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