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ONOO<-> spectroscopic probe, preparation method thereof and method for measuring ONOO<-> by ultraviolet visible absorption spectrum and fluorescence spectrum

A fluorescent probe and probe technology, applied in fluorescence/phosphorescence, color/spectral characteristic measurement, chemical instruments and methods, etc., can solve the problems of unfavorable rapid detection and analysis, cumbersome operation process, low sensitivity, etc., and achieve cheap and easy reagents Obtaining, quick and easy operation, high sensitivity effect

Active Publication Date: 2019-07-05
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Colorimetric detection of ONOO is now possible - The fluorescent probes are very few, and in the prior art, the detection of ONOO - However, there are problems of low sensitivity, poor accuracy, cumbersome operation process, and poor recognition, which is not conducive to rapid detection and analysis.

Method used

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  • ONOO&lt;-&gt; spectroscopic probe, preparation method thereof and method for measuring ONOO&lt;-&gt; by ultraviolet visible absorption spectrum and fluorescence spectrum
  • ONOO&lt;-&gt; spectroscopic probe, preparation method thereof and method for measuring ONOO&lt;-&gt; by ultraviolet visible absorption spectrum and fluorescence spectrum
  • ONOO&lt;-&gt; spectroscopic probe, preparation method thereof and method for measuring ONOO&lt;-&gt; by ultraviolet visible absorption spectrum and fluorescence spectrum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Synthesis and characterization of embodiment 1 fluorescent probe L

[0029] Fluorescent probe L was obtained by heating 1,2-diaminoanthraquinone and quinoline-2-carbaldehyde in a methanol solution at 65 °C for 24 h under reflux, then cooling, suction filtration, washing and drying.

[0030] 1H NMR (600 MHz, DMSO) δ 8.52 (s, 1H), 8.23 ​​(d, J = 22.1 Hz, 1H), 8.20 (s, 1H), 8.11 (s, 1H), 7.99 (s, 1H), 7.88 (s, 1H), 7.80 (s, 1H), 7.74(s, 1H), 7.66 – 7.62 (m, 1H), 7.62 – 7.55 (m, 1H), 7.47 (s, 1H), 6.78 (s,1H ), 6.38 (s, 1H), 3.33 (s, 2H). HRMS (ESI): calcd. for C 24 h 15 N 3 o 2 (L+H) 378.12, found 378.12344.

[0031] The reaction formula is:

[0032] .

Embodiment 2

[0033] Embodiment 2 fluorescent probe L is used for ONOO - UV absorbance determination

[0034] Take 2 mL of DMSO:phosphate buffer solution (4:1) containing 1 mM fluorescent probe and add it to a clean colorimetric tube, and detect it on a UV-Vis spectrophotometer, with ONOO - With the addition of , the color of the solution changed from pink to golden yellow, and a new absorption peak appeared at 477 nm; with ONOO - With the increase, the maximum absorbance detected on the UV-Vis spectrophotometer gradually shifted to the left and enhanced to see figure 1 .

Embodiment 3

[0035] Embodiment 3 fluorescent probe L is used for ONOO - Fluorescence spectrometry

[0036] Take 2 mL of DMSO:Phosphate buffer solution (4:1) containing 1 mM fluorescent probe and add it to a clean colorimetric tube, and detect it on a fluorescence photometer, with ONOO - The addition of , the emission peak at 632 nm disappeared, and a new absorption peak gradually appeared at 590 nm; with ONOO - The increase of the maximum absorption peak detected on the fluorescence photometer gradually shifted to the left and enhanced see figure 2 .

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Abstract

The invention discloses a spectroscopic probe for detecting peroxynitrite ion (ONOO<->), a preparation method of the spectroscopic probe, and a method for measuring the ONOO<-> by an ultraviolet visible absorption spectrum and a fluorescence spectrum, and belongs to the technical field of ONOO<-> detection. The synthetic method of the ONOO<-> spectroscopic probe comprises the following steps: performing heating reflux on 1,2-diaminoanthraquinone and quinoline-2-formaldehyde in a methanol solution, performing suction filtration, performing washing, and performing drying to obtain the spectroscopic probe. The ONOO<-> probe provided by the invention can quickly detect the ONOO<-> in a high-selectivity high-sensitivity manner, and the method is simple and convenient to operate.

Description

technical field [0001] The present invention relates to ONOO - The technical field of preparation and detection, specifically relates to a kind of ONOO - Spectral probe and preparation method, and UV-visible absorption spectrum, fluorescence spectrum determination of ONOO - Methods. Background technique [0002] ONOO - It is a strong oxidant that acts on macromolecules such as enzymes, proteins, lipids, and DNA to produce cytotoxic effects, causing cell metabolism disorders and energy depletion, leading to cell apoptosis, damage, and even death. [0003] Colorimetric detection of ONOO is now possible - The fluorescent probes are very few, and in the prior art, the detection of ONOO - However, there are problems of low sensitivity, poor accuracy, cumbersome operation process, and poor identification, which is not conducive to rapid detection and analysis. Contents of the invention [0004] The object of the present invention is to provide a kind of preparation and det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D215/12C09K11/06G01N21/33G01N21/64
CPCC07D215/12C09K11/06G01N21/33G01N21/6428G01N21/643C09K2211/1011C09K2211/1029
Inventor 李明璐宋胜梅梁帆李甘董川双少敏
Owner SHANXI UNIV
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