High-flux drug detection micro-fluidic chip and application
A microfluidic chip, high-throughput technology, applied in biological testing, material inspection products, laboratory containers, etc., can solve the problem of large sample demand, achieve good detection results, and achieve high-throughput and fast results Detection analysis, the effect of strong adsorption capacity
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[0023] Example 1 Fabrication of chips
[0024] see figure 1 , a microfluidic chip that can realize high-throughput drug detection. It consists of a substrate layer 1, a modification layer 2, and a reaction layer 3. The reaction layer 3 consists of 24 identical reaction modules 4. Each reaction module 4 consists of The inlet 5, the reaction channel 6 and the outlet 7 are constituted.
[0025] The substrate layer 1 and the modification layer 2 use commercially available glass slides that have been coated with polylysine, preferably with a thickness of 1 mm, a width of 25 mm, and a length of 77 mm.
[0026] The reaction layer 3 adopts PDMS as a material, adopts multi-layer soft lithography technology to make a mold with a micro-channel structure, and pours PDMS with breathable properties on the mold to cure and release the mold. The thickness of the reaction layer 3 can be adjusted according to actual needs, preferably 3-5mm.
[0027] After the reaction layer 3 is made, punch ...
Example Embodiment
[0028] Example 2 Preparation of the standard curve for the detection of methamphetamine (see figure 2 )
[0029] (1) Dilute the complete methamphetamine antigen to 200 μg / mL with 0.01 mol / L, pH7.4 phosphate buffered saline (PBS), and use a pipette to add 3 μL from each inlet 5 into the reaction channel 6 . The 3 reaction modules in the negative control area 8 were only fed with PBS. The chips were dried at 37°C overnight.
[0030] (2) Tween-20 with a volume fraction of 0.05% was added to PBS to prepare a PBST coating solution. Pipette 3 μL of coating solution from each inlet 5 into reaction channel 6. The chips were placed in a wet box and kept at 37°C for 1 h.
[0031] (3) 15 μL of PBST was added to each inlet 5 , and the PBST was drawn out from the outlet 7 using a syringe pump with a pulling function.
[0032] (4) Use PBST to prepare 2.4 μg / mL fluorescent latex microsphere solution with methamphetamine antibody.
[0033] (5) Use the mixture prepared in (4) to seriall...
Example Embodiment
[0037] Example 3 Ice drug detection
[0038](1) Dilute the complete methamphetamine antigen to 200 μg / mL with 0.01 mol / L PBS with pH 7.4, and use a pipette to add 3 μL from each inlet 5 into the reaction channel 6. The chips were dried at 37°C overnight.
[0039] (2) Tween-20 with a volume fraction of 0.05% was added to PBS to prepare a PBST coating solution. Pipette 3 μL of coating solution from each inlet 5 into reaction channel 6. The chips were placed in a wet box and kept at 37°C for 1 h.
[0040] (3) 15 μL of PBST was added to each inlet 5 , and the PBST was drawn out from the outlet 7 using a syringe pump with a pulling function.
[0041] (4) Use PBST to prepare 2.4 μg / mL fluorescent latex microsphere solution with methamphetamine antibody.
[0042] (5) Use the mixture prepared in (4) to mix with the methamphetamine sample.
[0043] (6) Add the methamphetamine sample obtained in (5) to the inlet 5, and pump it into the reaction channel 6 from the outlet 7 through a...
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