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High-flux drug detection micro-fluidic chip and application

A microfluidic chip, high-throughput technology, applied in biological testing, material inspection products, laboratory containers, etc., can solve the problem of large sample demand, achieve good detection results, and achieve high-throughput and fast results Detection analysis, the effect of strong adsorption capacity

Pending Publication Date: 2017-09-29
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the colloidal gold test paper detection method can be used for rapid on-site detection, a single test paper can only detect a single sample and a single drug, and each test paper can only read the test results of a single test paper
At the same time, due to the variety of poisons, the demand for samples is also very large

Method used

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  • High-flux drug detection micro-fluidic chip and application
  • High-flux drug detection micro-fluidic chip and application
  • High-flux drug detection micro-fluidic chip and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Fabrication of Chips

[0024] see figure 1 , a microfluidic chip capable of high-throughput drug detection. It consists of a substrate layer 1, a modification layer 2, and a reaction layer 3. The reaction layer 3 is composed of 24 identical reaction modules 4, and each reaction module 4 consists of An inlet 5, a reaction channel 6 and an outlet 7 are formed.

[0025] The substrate layer 1 and the modification layer 2 are commercially available glass slides coated with poly-lysine, preferably with a thickness of 1 mm, a width of 25 mm, and a length of 77 mm.

[0026] The reaction layer 3 is made of PDMS, and a mold with a microchannel structure is made by multi-layer soft lithography technology, and the gas-permeable PDMS is poured on the mold to be cured and demoulded. The thickness of the reaction layer 3 can be adjusted according to actual needs, preferably 3-5mm.

[0027] After the reaction layer 3 is finished, use a hole puncher with a diameter of 1mm t...

Embodiment 2

[0028] Embodiment 2 The making of methamphetamine detection standard curve (see figure 2 )

[0029] (1) Dilute the complete antigen of methamphetamine to 200 μg / mL with 0.01 mol / L, pH7.4 phosphate buffer solution (PBS), and use a pipette gun to add 3 μL from each inlet 5 into the reaction channel 6. The 3 reaction modules in the negative control area 8 are only fed with PBS. Chips were dried overnight at 37°C.

[0030] (2) Add Tween-20 with a volume fraction of 0.05% in PBS to prepare a PBST coating solution. Add 3 μL of the coating solution from each inlet 5 into the reaction channel 6 with a pipette gun. Put the chips into a wet box and keep at 37°C for 1h.

[0031] (3) 15 µL of PBST was added to each inlet 5, and PBST was drawn out from the outlet 7 using a syringe pump having a suction function.

[0032] (4) Use PBST to configure 2.4 μg / mL fluorescent latex microsphere solution with methamphetamine antibody.

[0033] (5) Use the mixture prepared in (4) to gradiently...

Embodiment 3

[0037] Example 3 Detection of methamphetamine

[0038](1) Dilute the complete antigen of methamphetamine to 200 μg / mL with 0.01 mol / L, pH7.4 PBS, and add 3 μL from each inlet 5 into the reaction channel 6 using a pipette gun. Chips were dried overnight at 37°C.

[0039] (2) Add Tween-20 with a volume fraction of 0.05% in PBS to prepare a PBST coating solution. Add 3 μL of the coating solution from each inlet 5 into the reaction channel 6 with a pipette gun. Put the chips into a wet box and keep at 37°C for 1h.

[0040] (3) 15 µL of PBST was added to each inlet 5, and PBST was drawn out from the outlet 7 using a syringe pump having a suction function.

[0041] (4) Use PBST to configure 2.4 μg / mL fluorescent latex microsphere solution with methamphetamine antibody.

[0042] (5) Use the mixture prepared in (4) to mix with the methamphetamine sample.

[0043] (6) Add the methamphetamine sample obtained in (5) to the inlet 5, and draw it into the reaction channel 6 from the ou...

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Abstract

The invention provides a high-flux drug detection micro-fluidic chip. The high-flux drug detection micro-fluidic chip consists of a substrate layer, a modification layer and a reaction layer, wherein the reaction layer consists of n reaction modules; each reaction module consists of an entrance, a reaction channel and an outlet. The chip modified by polylysine is hydrophilic, facilitates a sample to be detected to flow into the chip, and has an adsorption effect on drug complete antigens; the polylysine of the modification layer is matched with PDMS (Polydimethylsiloxane) of the reaction layer, so that the hydrophilic feature of the polylysine can be combined with the high-protein adsorption feature of the PDMS to achieve a better detection effect; detection of different samples occurs in different reaction channels, so that the phenomenon of mutual interference is avoided, and non-specific adsorption is lowered; a single chip is provided with a plurality of reaction modules to realize high-flux rapid result detection and analysis; the sizes, intervals and number of the reaction modules can be adjusted as required to realize simultaneous field detection of a plurality of samples and drugs, so that different detection requirements are met.

Description

technical field [0001] The invention belongs to the detection field of prohibited drugs, and relates to a microfluidic chip device for high-throughput drug detection and its application technology. Background technique [0002] Although chemicals such as morphine, methamphetamine, and ecstasy (MDMA) were originally developed to treat human diseases and relieve human pain, they have serious side effects and are addictive after use, so It is also included in the drug list. The abuse of drugs has brought great impact and harm to people's life and life safety. [0003] The current drug detection methods mainly include the colloidal gold test paper detection in the early stage and the gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry methods used in the later court detection. Although the colloidal gold test paper detection method can be detected quickly on the spot, a single test paper can only detect a single sample and a single drug, and each c...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/53
CPCG01N33/53B01L3/502707B01L2300/0861
Inventor 牟颖宋祺孙敬敬李文放丁国荣
Owner ZHEJIANG UNIV
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