111 results about "Non specific adsorption" patented technology

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

The invention relates to the technical field of biological detection, and provides a kit and a method for rapidly detecting a platelet antibody by utilizing a phytolectin solid-phase adsorption method. The detection kit provided by the invention comprises a reaction plate which is coated with phytolectin and adsorbs platelets. According to the method, the platelet of multiple persons is adsorbed by utilizing the non-specific adsorption capacity of the phytolectin, and the adsorbed platelet is directly freeze-dried to the bottom of a microwell plate. During use, a detection result can be obtained only by adding detected plasma / serum into the reaction plate for incubation and then adding an anti-human globule reagent and indicating red blood cells, so that intermediate links of detection are reduced, and standardization and automation of detection are facilitated.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention discloses a multi-nucleic acid probe rapid modification and multi-target detection device based on electric field regulation and control. An instrument main body in the device is integrated with an automatic liquid path system and a micro electrochemical workstation module, and is used for realizing programmable reagent and sample conveying and multi-channel electrochemical measurement. The detachable sensing chip is formed by bonding a microelectrode array chip and a micro-fluidic chip, and is switched through a PCB (Printed Circuit Board) to realize plug and play. According to the present invention, the positive suction-negative discharge strategy of the electric field is utilized, the preferred positive potential is applied to the target electrode to efficiently attract the probe, and the preferred negative potential is applied to the non-target electrode to inhibit the non-specific adsorption so as to achieve the spatial selective rapid fixation and the accurate density regulation and control of the multi-nucleic acid probe in the extremely short time; the device can be used for quantitatively detecting various body fluid disease markers in a plurality of urine samples at the same time in single operation, and an efficient, accurate and portable multi-marker comprehensive analysis solution is provided for early screening and instant diagnosis of tumors.

This invention discloses a method and sensor for adiponectin detection using a polyaniline and polyacrylic acid-modified wire mesh carbon electrode, relating to the field of adiponectin detection technology. The method involves electrochemically activating the wire mesh carbon electrode, electropolymerizing it to form a polyaniline-polyacrylic acid composite modification layer, immobilizing adiponectin monoclonal antibody after activation with N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, blocking the antibody with bovine serum albumin, and reacting it with a serum sample. The adiponectin concentration is determined based on the peak potential shift and peak current change. This invention simultaneously achieves both conductive transport and antibody immobilization, reduces non-specific adsorption and serum interference, and improves detection sensitivity, repeatability, and quantitative accuracy.

A method for measuring the concentration of an analyte on the basis of a periodically variable external regulation force, and an oscillator system, which aim to solve the problem of non-specific adsorption in existing immunoassay techniques leading to reduced sensitivity and specificity in the measurement of analyte concentrations in samples. The measurement method comprises: obtaining a detection system, and setting a periodically variable external regulation force; binding both a capture probe and a detection probe with an analyte in a sample; making labeled particles, which are bound to the detection probe, perform periodic reciprocating motion under the action of the external regulation force; and by means of a monitoring and imaging apparatus, recording motion imaging signals of the labeled particles to select specifically bound molecules, and compiling statistics to calculate the proportion of the specifically bound molecules so as to obtain the analyte concentration. A specifically bound analyte can be accurately selected, thereby enhancing the capability and detection sensitivity of specific detection of analytes. The method has no complex elution processes, has a short detection response time, and exhibits high-sensitivity detection capability in complex environments.

The invention belongs to the technical field of biomedical detection, and particularly discloses a nucleic acid aptamer for specifically recognizing serum alpha fetoprotein and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer comprises a sequence as shown in SEQ ID No.1, or has more than 90% of homology with the sequence as shown in SEQ ID No.1, or one or more nucleotides are deleted or added in the sequence as shown in SEQ ID No.1. The nucleic acid aptamer can realize specific efficient recognition of AFP protein, also shows extremely high selectivity in a complex serum sample environment, eliminates non-specific adsorption interference of homologous protein and other serum interference protein, greatly reduces the false positive rate, and improves the accuracy and specificity of clinical detection. And the material constructed by directionally functionalizing the aptamer on the surface of the magnetic nano-carrier has rapid magnetic response performance, separation and enrichment of target protein can be realized within seconds, and the clinical diagnosis time is greatly shortened.

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

The invention relates to the technical field of traditional Chinese medicinal material detection, and particularly discloses an alpha-asarone extracting solution detection method and system based on a biosensor, and the method sequentially comprises the following steps: mixing medicinal material powder to be detected with an extraction solvent according to a certain solid-to-liquid ratio, and extracting volatile oil by adopting an ultrasonic or reflux method to obtain a crude extracting solution; volatile oil concentrated mother liquor is obtained; obtaining a standard solution; a combined-state electrode is obtained; obtaining electrochemical response data; and converting the electrochemical response data into the content of alpha-asarone in the sample according to the standard curve, and evaluating the precision, the recovery rate and the regeneration performance to obtain a final detection result. Recognition sites prepared by adopting the molecularly imprinted polymer are complementarily paired with template molecules in steric configuration, steric hindrance and key functional groups to realize specific recognition of alpha-asarone, so that false signals caused by matrix interference and non-specific adsorption are remarkably reduced; the electrochemical measurement method is extremely sensitive to electron transfer generated by surface bonding.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The application discloses an acridinium ester labeled complex, which comprises a body, and a labeling group labeled on the body, wherein the body is an antibody, and the labeling group is an acridinium ester group. A preparation method of the acridinium ester labeled complex comprises the following steps: sucking a labeling buffer into a centrifugal tube; sucking an antibody to be labeled into the centrifugal tube and mixing to generate a first mixed solution; adding an acridinium ester solution and mixing, and then performing reaction at 25 DEG C in the dark for 2 hours to generate a second reaction solution; adding a termination buffer and mixing, and then performing reaction at 25 DEG C in the dark for 30 minutes to generate a third reaction solution; performing desalting purification on the third reaction solution to generate a fourth mixed solution; and filtering the fourth mixed solution with a 0.22 mu m filter membrane to generate the acridinium ester labeled complex. According to the preparation method, the labeling efficiency of the acridinium ester and the antibody is higher, the reactivity is stronger, the non-specific adsorption is lower, the sensitivity is higher, the repeatability and stability are better, and the labeling method is simple, rapid and easy to repeat.

The invention relates to the technical field of N sugar chain detection, and particularly discloses an adsorption material for purifying an N sugar chain, a hydrophilic magnetic sphere containing the adsorption material and a kit. The adsorption material for purifying the N sugar chain, provided by the invention, comprises synthesized 1-fluorobetaine acyl chloride hydrochloride which is a hydrophilic molecule and can be connected to a magnetic microsphere through a bonding effect, so that a new hydrophilic magnetic sphere is obtained. The hydrophilic magnetic sphere combined with the hydrophilic molecule can increase the N sugar chain grabbing efficiency of the magnetic sphere and reduce non-specific adsorption to other molecules such as free dye. When being used for N-sugar spectrum detection of an antibody sample, the N-sugar spectrum detection kit is very good in repeatability, precision, linearity and accuracy indexes.

The invention discloses a strong cation exchange chromatography medium as well as a preparation method and application thereof, and belongs to the technical field of biological separation and purification. The structural formula of the strong cation exchange chromatography medium is shown in the specification, the preparation method comprises the following steps: (1) synthesizing epoxy porous polymer microspheres; (2) carrying out an epoxy-sulfydryl click reaction with sulfydryl sulfonate; and (3) hydrolyzing the residual epoxy group to obtain the epoxy resin. The strong cation exchange chromatography medium has the advantages of high protein loading capacity, low non-specific adsorption, good mechanical property and the like, the preparation process is simple, special hydrophilic modification is not needed, and the process is simplified, the industrial production period is shortened and the production cost is reduced under the condition of meeting the conventional protein elution condition. And meanwhile, the high efficiency of the click reaction can meet the characteristics of high loading capacity, good biocompatibility and the like of the chromatography medium.

The invention relates to a modified luminous particle as well as a preparation method and application thereof. The modified light-emitting particles comprise light-emitting particles and zwitterionic polymers adsorbed on the light-emitting particles, and the modified light-emitting particles can react with active oxygen to generate detectable chemiluminescence signals. According to the scheme provided by the invention, the repulsive force between particles and the non-specific adsorption resistance can be improved, so that the specificity of the light-excited chemiluminescence detection reagent is improved, and the accuracy of a detection result is ensured.

A random terpolymer of N-(2-hydroxypropyl) methacrylamide, carboxybetaine methacrylamide and sulfobetaine methacrylamide, and a polymer brush and to a functionalized polymer brush containing this terpolymer are disclosed. The random terpolymer increases the resistance of the substrate surface to non-specific adsorption of substances from biological media and / or to non-specific interaction with biological media components, and is suitable for use in the form of a polymer brush, for example in sensors or membranes.

The invention is applicable to the technical field of biological detection, and provides a method for detecting staphylococcus aureus based on combination of a screen-printed electrode and CRISPR / Cas13a, a probe containing a DNA / RNA hybrid chain is combined with a magnetic bead to form a simple composite structure, when the Cas13a recognizes 16sRNA of S. aureus, trans-cleavage activity can be immediately activated, the probe chain is rapidly cut off, the cut-off amount depends on the activated amount, and when the Cas13a recognizes 16sRNA of S. aureus, the Cas13a can be used for detecting staphylococcus aureus. And after transient magnetic separation, a result is observed on the screen-printed electrode. The method has ultrahigh specificity: the CRISPR / crRNA sequence ensures that staphylococcus aureus and other microorganisms can be accurately distinguished; the trans-cutting of Cas13a provides chemical amplification, and the magnetic separation purification greatly reduces background signals caused by non-specific adsorption, so that the signal-to-noise ratio and the sensitivity are remarkably improved.

This application discloses a chemiluminescence detection kit for acrylonitrile magnetic microparticles and its detection method. The kit includes a magnetic bead working solution and a luminescent label. The magnetic bead working solution includes acrylonitrile monoclonal antibody A and streptavidin. The luminescent label includes acrylonitrile monoclonal antibody B, modified nano-silica, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N,N-dimethylformamide, acrylonitrile succinimide ester, glutaraldehyde, and ethanolamine. This application uses modified nano-silica as a carrier and grafts a polyethylene glycol hydrophilic layer onto its surface, which amplifies the acrylonitrile ester molecule signal, reduces non-specific adsorption, and improves the sensitivity, specificity, and reliability of the detection.

The application discloses a fast and efficient chemical proteomics sample preparation method, relates to the field of proteomics, and designs and synthesizes agarose microspheres coated with a cleavable linker compound. The free end of the cleavable linker compound contains an azido group, which has good compatibility with existing alkyne probes. Through one-step efficient click chemistry reaction, the probe-labeled peptide segment can be specifically captured on the surface of the agarose microspheres. Since the agarose microspheres and the peptide segment are covalently connected, very strong denaturants or surfactants can be used to wash the medium to remove non-specific adsorption as much as possible. Finally, the probe-labeled peptide segment can be efficiently dissociated from the agarose microspheres through cleavage treatment. The sample preparation method in the application simplifies the cumbersome "click chemistry and streptavidin enrichment" into one-step fast "click chemistry" reaction, and greatly simplifies the operation process.

The invention discloses a magnetic particle chemiluminescence detection kit and a detection method thereof, the kit comprises a magnetic bead working solution and a luminescent marker, the magnetic bead working solution comprises a peptin monoclonal antibody A and streptavidin, the luminous marker is prepared from a peptin monoclonal antibody B, modified nano silicon dioxide, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N, N-dimethylformamide, acridine succinimide ester, glutaraldehyde and ethanolamine. The acridinium ester molecular signal can be amplified by taking the modified nano silicon dioxide as a carrier and grafting the polyethylene glycol hydrophilic layer on the surface, the non-specific adsorption can be reduced, and the effects of improving the sensitivity, specificity and reliability of detection are achieved.

The present invention provides a particle that shows a small non-specific adsorption, has a reactive functional group for chemically bonding a ligand thereto, and is suitable for an agglutination method. The particle includes a polymer having a unit having a side chain A and a unit having a side chain B, wherein the side chain A has a carboxyl group at a terminal thereof, and has an ester bond and an amide bond in a molecular structure thereof; the side chain B has an alkyl group at a terminal thereof; and a zeta potential at the time when the particles are dispersed in water is minus 40 mV or higher and minus 5 mV or lower.

The invention relates to the technical field of biology, in particular to a magnetic microsphere, a preparation method and application thereof and a reagent containing the magnetic microsphere. The preparation method comprises the following steps: firstly, preparing carboxyl-containing polymer microspheres by a one-step method, then carrying out heat treatment under an alkaline condition, and then carrying out THF oscillation to finally prepare porous polystyrene carboxyl microspheres with uniform pore channels, meanwhile, avoiding using divinyl benzene as a cross-linking agent, reducing the luminous background of the finally obtained magnetic microspheres, and improving the luminous efficiency of the magnetic microspheres. The method can be better applied to the field of chemiluminescence immunoassay. Due to the existence of a surface porous structure and carboxyl, the stability and the functionalization degree of the magnetic material are improved, the problems of magnetic leakage and magnetic shell layer falling are avoided, the surface of the porous ball is smoother and more uniform, and a solid foundation is laid for the subsequent coating process and reduction of non-specific adsorption of the surface of the magnetic ball; the magnetic ball is prepared by using a grinding method, and has the advantages of safety, no toxicity, easiness in operation, no pollution and easiness in industrial popularization.

The present application relates to the field of micro-nano magnetic material for in vitro detection, and particularly relates to a preparation method of high-loading streptavidin magnetic microspheres, the magnetic microspheres and application thereof; in the preparation process, first, streptavidin is reacted with a crosslinking agent to obtain polymerized streptavidin, and then the polymerized streptavidin is coupled on the surface of the magnetic microspheres to obtain streptavidin magnetic microspheres; wherein the structure of the crosslinking agent comprises a hydrophilic flexible chain. The present application has the effects of improving the loading and stability of streptavidin on the surface of the magnetic microspheres, reducing non-specific adsorption, and enhancing the sensitivity of detection.

The invention discloses a self-calibration functionalization strategy based on a CNT FET sensor, and relates to the technical field of CNT FET sensor calibration. According to the self-calibration functionalization strategy based on the CNT FET sensor, the same-chip multi-channel integration is realized through local differentiation functionalization compatible with a micro-nano process, and the self-calibration target in the device is finally achieved by combining the physical and chemical barrier effect of a hydrogel three-dimensional anti-fouling layer; high stability, high specificity, high reliability and strong anti-interference capability of detection in a complex practical application scene are guaranteed, the defects that in the prior art, the operation process for eliminating influences is complex, and real-time drifting of a device cannot be eliminated are successfully overcome, and the defects that due to an existing modification method, non-specific adsorption is caused, and same-piece contrast cannot be achieved are overcome. And the stability and reliability of the detection process cannot be ensured.

The application discloses a method for constructing a high-throughput protein capture interface by antibody adsorption, which comprises the following steps: S1, synthesizing a tetrahedral framework nucleic acid structure; S2, mixing a capture antibody and the synthesized tetrahedral framework nucleic acid structure on a gold island substrate to prepare a high-throughput protein capture interface; S3, placing the gold island substrate in a wet box for overnight incubation; S4, blocking the capture interface to prevent non-specific adsorption; S5, incubating a protein target; S6, incubating a detection antibody; S7, incubating a fluorescent secondary antibody; S8, washing the capture interface with pure water and centrifuging and air-drying; and S9, performing fluorescent imaging and quantification on the capture interface by using a chip scanner. The method for constructing a high-throughput protein capture interface by antibody adsorption has high uniformity, and can realize high-throughput, high-sensitivity and high-accuracy protein capture and quantitative detection.

This invention discloses a field-effect transistor biosensor based on a framework nucleic acid-gold nanoparticle composite structure and its fabrication method, comprising the following steps: 1) providing a carbon nanotube field-effect transistor substrate; 2) depositing a hafnium oxide dielectric layer on the surface of the carbon nanotube channel; 3) depositing gold nanoparticles on the surface of the hafnium oxide dielectric layer; 4) preparing a tetrahedral framework nucleic acid probe; 5) adding the probe solution to the surface of the gold nanoparticles for incubation and fixation; 6) sealing unreacted sites on the surface of the gold nanoparticles and washing with buffer solution. This invention achieves precise interface modification through atomic layer deposition, electron beam evaporation, and gold-sulfur bond anchoring. It utilizes the rigid structure of the tetrahedral framework nucleic acid to achieve precise, directional, and spaced probe distribution, effectively overcoming Debye shielding limitations and reducing non-specific adsorption. The sensor exhibits ultra-high sensitivity, excellent stability and reusability, strong specificity, and good versatility, and can be used for highly sensitive and specific detection of various targets such as nucleic acids and proteins.

The application discloses a reagent containing pig pseudorabies virus gB protein magnetic microparticle coating, a preparation method and a kit, and relates to the technical field of antibody detection. The preparation method of the reagent containing pig pseudorabies virus gB protein magnetic microparticle coating comprises the following steps: incubating magnetic microparticles and gB protein antibodies in a coating buffer solution; removing the reaction solution, and performing first sealing on the gB protein magnetic microparticle coating with a first sealing solution; after the first sealing is completed, the first sealing solution is washed away, and then the gB protein magnetic microparticle coating is sealed for the second time with a second sealing solution; after the second sealing is completed, the second sealing solution is washed away, and then resuspension is performed to obtain the reagent containing the gB protein magnetic microparticle coating. The two-step sealing method is adopted to prepare the reagent containing the pig pseudorabies virus gB protein magnetic microparticle coating, the sealing effect is good, and non-specific adsorption in the system is reduced. The kit provided by the application contains the reagent, and has the advantages of high analysis sensitivity, a wide linear range and high accuracy.

This invention relates to the field of protein extraction technology, and in particular to a highly efficient method for extracting and preserving natural BICC1 protein from serum, comprising the following steps: 1) preparation of aminated magnetic Fe3O4 nanoparticles; 2) preparation of Fe3O4@Au nanoparticles; 3) preparation of BICC1 template protein; 4) binding of BICC1 template protein; 5) immobilization of BICC1 template protein; 6) elution of BICC1 template protein to prepare BICC1 magnetic molecularly imprinted polymer microspheres; 7) purification of natural BICC1 protein. This invention features a simple method with mild reaction conditions. The colloidal gold layer of the magnetic molecularly imprinted polymer achieves highly efficient adsorption of template molecules, and the outermost polymer immobilization layer, composed of neutral epoxy groups, reduces non-specific adsorption, enabling efficient and specific extraction of natural BICC1 protein directly from serum.

The invention relates to the technical field of biology, in particular to a bispecific antibody targeting IgE and IL-13 and application of the bispecific antibody. The invention provides a bispecific antibody targeting IgE and IL-13, the bispecific antibody has high specificity on IgE and IL-13 adsorption, generates an equivalent combination effect or a better synergistic effect of two antibodies, and enriches treatment means of diseases caused by abnormality of IgE and IL-13. The immunoadsorbent prepared by adopting the bispecific antibody disclosed by the invention can adsorb IgE and IL-13 in blood with high specificity, has low non-specific adsorption on IgG, IgA and IgM, and has great clinical application potential.