173 results about "Non specific adsorption" patented technology

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The invention relates to the technical field of immunodetection and immunodiagnosis, in particular to a chemiluminescence substrate solution, a chemiluminescence detection kit and a detection method. The chemiluminescent substrate solution comprises a substrate solution 1, or comprises the substrate solution 1 and a substrate solution 2, the substrate solution 1 comprises sodium hydroxide, a surfactant and a blue light absorbent for absorbing blue light with the wavelength of 400-500 nm; the substrate solution 2 comprises inorganic acid and hydrogen peroxide. According to the application, the blue light absorbent is added into the chemiluminescent substrate liquid, so that blue light emitted by acridinium ester can be absorbed, a luminescent signal is reduced, and particularly, the luminescent value of a background caused by non-specific adsorption is reduced, so that the signal-to-noise ratio is increased.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

To provide a resin particle that is resistant to non-specific adsorption when used for detecting a target substance such as an antigen, in particular, to provide a resin particle exhibiting high sensitivity when detecting the target substance using a fluorescence depolarization method.SOLUTION: A resin particle has a europium complex, wherein the resin particle has a core-shell structure, wherein the resin particle has a core portion that contains a specific copolymer, and the resin particle has a shell portion that contains a specific polymer, wherein at least one of the core portion and the shell portion has a crosslinked structure.SELECTED DRAWING: Figure 1

The invention relates to the technical field of biological detection, and provides a kit and a method for rapidly detecting a platelet antibody by utilizing a phytolectin solid-phase adsorption method. The detection kit provided by the invention comprises a reaction plate which is coated with phytolectin and adsorbs platelets. According to the method, the platelet of multiple persons is adsorbed by utilizing the non-specific adsorption capacity of the phytolectin, and the adsorbed platelet is directly freeze-dried to the bottom of a microwell plate. During use, a detection result can be obtained only by adding detected plasma / serum into the reaction plate for incubation and then adding an anti-human globule reagent and indicating red blood cells, so that intermediate links of detection are reduced, and standardization and automation of detection are facilitated.

The invention relates to the technical field of filler preparation, and discloses an analysis filler for separating glycosylated hemoglobin and a preparation method thereof.The analysis filler for separating glycosylated hemoglobin is obtained by synthesizing nonporous Polymer microspheres of 5 micrometers through a dispersion polymerization method, conducting hydrophilic treatment on the surfaces of the Polymer microspheres to obtain Polymer base balls and finally introducing ion exchange groups to the Polymer base balls. The hydrophilicity of the surface of the matrix is enhanced through hydrophilization treatment, non-specific adsorption is reduced, meanwhile, the swelling property of the filler in water is not affected, and the mechanical strength is not affected. The glycosylated hemoglobin analysis filler which is uniform in granularity, good in separation effect and long in service life can be obtained through the preparation method of the analysis filler.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention discloses a high-hydrophilicity macroporous microsphere and a preparation method thereof, and the preparation method comprises the following steps: firstly, preparing a polystyrene seed microsphere and swelling; styrene, divinyl benzene and vinyl ester monomers are added for a polymerization reaction, then alcoholysis is carried out, and macroporous microspheres with primary hydrophilic groups on the surfaces are obtained; and finally, modifying a secondary hydrophilic group to obtain the high-hydrophilicity macroporous microsphere. The high-hydrophilicity macroporous microsphere provided by the invention is excellent in comprehensive performance, the surface of the microsphere is completely covered with the high-hydrophilicity macroporous microsphere through primary and secondary hydrophilic groups, and the hydrophilic groups are directly connected with the microsphere through chemical bonds, so that the high-hydrophilicity macroporous microsphere has good stability and can effectively reduce non-specific adsorption of base spheres and improve the recovery rate of protein purification; the preparation method of the high-hydrophilicity macroporous microspheres provided by the invention is simple and efficient, has wide raw material sources, can be used for preparing fillers such as affinity chromatography fillers, ion chromatography fillers, composite mode chromatography fillers and the like, and has wide application prospects and good industrialization conditions.

The invention relates to the field of phosphorylated tau protein detection, in particular to a method for improving phosphorylated tau protein detection sensitivity and a kit thereof. The invention provides a method for improving phosphorylated tau protein detection sensitivity. The method comprises the following steps: S101, obtaining a blood sample; s102, adding the detection reagent into the blood sample; s103, detecting the concentration of the phosphorylated tau protein in the blood sample by using a chemiluminescence method; wherein the detection reagent comprises an alkaline phosphatase polymer-antibody compound. The alkaline phosphatase polymer-antibody compound prepared by the invention is high in yield, good in stability, high in coupling efficiency, low in background, good in precision, strong in anti-interference capability, smaller in batch difference and lower in non-specific adsorption, the sensitivity is remarkably improved, and the compound shows remarkable advantages in detection of phosphorylated tau protein in a blood sample.

The invention belongs to the technical field of phospholipid membrane monolithic columns, and particularly relates to a preparation method and application of a bionic phospholipid membrane monolithic column for screening and separating antimicrobial peptides. The bionic phospholipid membrane monolithic column is prepared from phospholipid monomers or mixed phospholipids, and is directly prepared in a quartz capillary through an in-situ polymerization reaction, so that the monolithic column has richer phospholipid types and a more complex phospholipid membrane structure, a biological membrane environment can be more accurately simulated, and the separation efficiency and selectivity are improved; the obtained mixed phospholipid membrane monolithic column is relatively good in polymerization condition, is tightly combined with a capillary wall, is excellent in structural performance, can realize separation of alkyl benzophenone compounds, has non-specific adsorption resistance, can also be used for separation, purification and screening of antibacterial peptides, and has wide application prospects. The method has important significance, and solves the problems of low efficiency, high cost, activity loss and the like existing in the conventional antibacterial peptide screening and purifying method.

The invention discloses a multi-nucleic acid probe rapid modification and multi-target detection device based on electric field regulation and control. An instrument main body in the device is integrated with an automatic liquid path system and a micro electrochemical workstation module, and is used for realizing programmable reagent and sample conveying and multi-channel electrochemical measurement. The detachable sensing chip is formed by bonding a microelectrode array chip and a micro-fluidic chip, and is switched through a PCB (Printed Circuit Board) to realize plug and play. According to the present invention, the positive suction-negative discharge strategy of the electric field is utilized, the preferred positive potential is applied to the target electrode to efficiently attract the probe, and the preferred negative potential is applied to the non-target electrode to inhibit the non-specific adsorption so as to achieve the spatial selective rapid fixation and the accurate density regulation and control of the multi-nucleic acid probe in the extremely short time; the device can be used for quantitatively detecting various body fluid disease markers in a plurality of urine samples at the same time in single operation, and an efficient, accurate and portable multi-marker comprehensive analysis solution is provided for early screening and instant diagnosis of tumors.

This invention discloses a method and sensor for adiponectin detection using a polyaniline and polyacrylic acid-modified wire mesh carbon electrode, relating to the field of adiponectin detection technology. The method involves electrochemically activating the wire mesh carbon electrode, electropolymerizing it to form a polyaniline-polyacrylic acid composite modification layer, immobilizing adiponectin monoclonal antibody after activation with N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, blocking the antibody with bovine serum albumin, and reacting it with a serum sample. The adiponectin concentration is determined based on the peak potential shift and peak current change. This invention simultaneously achieves both conductive transport and antibody immobilization, reduces non-specific adsorption and serum interference, and improves detection sensitivity, repeatability, and quantitative accuracy.

A method for measuring the concentration of an analyte on the basis of a periodically variable external regulation force, and an oscillator system, which aim to solve the problem of non-specific adsorption in existing immunoassay techniques leading to reduced sensitivity and specificity in the measurement of analyte concentrations in samples. The measurement method comprises: obtaining a detection system, and setting a periodically variable external regulation force; binding both a capture probe and a detection probe with an analyte in a sample; making labeled particles, which are bound to the detection probe, perform periodic reciprocating motion under the action of the external regulation force; and by means of a monitoring and imaging apparatus, recording motion imaging signals of the labeled particles to select specifically bound molecules, and compiling statistics to calculate the proportion of the specifically bound molecules so as to obtain the analyte concentration. A specifically bound analyte can be accurately selected, thereby enhancing the capability and detection sensitivity of specific detection of analytes. The method has no complex elution processes, has a short detection response time, and exhibits high-sensitivity detection capability in complex environments.

The invention belongs to the technical field of biomedical detection, and particularly discloses a nucleic acid aptamer for specifically recognizing serum alpha fetoprotein and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer comprises a sequence as shown in SEQ ID No.1, or has more than 90% of homology with the sequence as shown in SEQ ID No.1, or one or more nucleotides are deleted or added in the sequence as shown in SEQ ID No.1. The nucleic acid aptamer can realize specific efficient recognition of AFP protein, also shows extremely high selectivity in a complex serum sample environment, eliminates non-specific adsorption interference of homologous protein and other serum interference protein, greatly reduces the false positive rate, and improves the accuracy and specificity of clinical detection. And the material constructed by directionally functionalizing the aptamer on the surface of the magnetic nano-carrier has rapid magnetic response performance, separation and enrichment of target protein can be realized within seconds, and the clinical diagnosis time is greatly shortened.

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

The invention relates to the technical field of traditional Chinese medicinal material detection, and particularly discloses an alpha-asarone extracting solution detection method and system based on a biosensor, and the method sequentially comprises the following steps: mixing medicinal material powder to be detected with an extraction solvent according to a certain solid-to-liquid ratio, and extracting volatile oil by adopting an ultrasonic or reflux method to obtain a crude extracting solution; volatile oil concentrated mother liquor is obtained; obtaining a standard solution; a combined-state electrode is obtained; obtaining electrochemical response data; and converting the electrochemical response data into the content of alpha-asarone in the sample according to the standard curve, and evaluating the precision, the recovery rate and the regeneration performance to obtain a final detection result. Recognition sites prepared by adopting the molecularly imprinted polymer are complementarily paired with template molecules in steric configuration, steric hindrance and key functional groups to realize specific recognition of alpha-asarone, so that false signals caused by matrix interference and non-specific adsorption are remarkably reduced; the electrochemical measurement method is extremely sensitive to electron transfer generated by surface bonding.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The invention belongs to the technical field of biological separation and purification, and particularly relates to a strong anion exchange chromatography filler as well as a preparation method and application thereof. The surface of the porous polyglycidyl methacrylate-divinylbenzene microsphere is modified with a layer of branched polyethyleneimine, so that the hydrophilic property of the material is greatly improved, and the porous polyglycidyl methacrylate-divinylbenzene microsphere has good biocompatibility; the high-density branched polyethyleneimine is added into the filler, non-specific adsorption caused by hydrophobic interaction in the protein separation and purification process is avoided, and meanwhile, the high-density branched polyethyleneimine is convenient for modifying a high-density quaternary ammonium group, so that the surface charge density of the filler is greatly improved, and the protein adsorption capacity of the filler is improved.

The application discloses an acridinium ester labeled complex, which comprises a body, and a labeling group labeled on the body, wherein the body is an antibody, and the labeling group is an acridinium ester group. A preparation method of the acridinium ester labeled complex comprises the following steps: sucking a labeling buffer into a centrifugal tube; sucking an antibody to be labeled into the centrifugal tube and mixing to generate a first mixed solution; adding an acridinium ester solution and mixing, and then performing reaction at 25 DEG C in the dark for 2 hours to generate a second reaction solution; adding a termination buffer and mixing, and then performing reaction at 25 DEG C in the dark for 30 minutes to generate a third reaction solution; performing desalting purification on the third reaction solution to generate a fourth mixed solution; and filtering the fourth mixed solution with a 0.22 mu m filter membrane to generate the acridinium ester labeled complex. According to the preparation method, the labeling efficiency of the acridinium ester and the antibody is higher, the reactivity is stronger, the non-specific adsorption is lower, the sensitivity is higher, the repeatability and stability are better, and the labeling method is simple, rapid and easy to repeat.

The invention relates to the technical field of N sugar chain detection, and particularly discloses an adsorption material for purifying an N sugar chain, a hydrophilic magnetic sphere containing the adsorption material and a kit. The adsorption material for purifying the N sugar chain, provided by the invention, comprises synthesized 1-fluorobetaine acyl chloride hydrochloride which is a hydrophilic molecule and can be connected to a magnetic microsphere through a bonding effect, so that a new hydrophilic magnetic sphere is obtained. The hydrophilic magnetic sphere combined with the hydrophilic molecule can increase the N sugar chain grabbing efficiency of the magnetic sphere and reduce non-specific adsorption to other molecules such as free dye. When being used for N-sugar spectrum detection of an antibody sample, the N-sugar spectrum detection kit is very good in repeatability, precision, linearity and accuracy indexes.

The invention discloses a strong cation exchange chromatography medium as well as a preparation method and application thereof, and belongs to the technical field of biological separation and purification. The structural formula of the strong cation exchange chromatography medium is shown in the specification, the preparation method comprises the following steps: (1) synthesizing epoxy porous polymer microspheres; (2) carrying out an epoxy-sulfydryl click reaction with sulfydryl sulfonate; and (3) hydrolyzing the residual epoxy group to obtain the epoxy resin. The strong cation exchange chromatography medium has the advantages of high protein loading capacity, low non-specific adsorption, good mechanical property and the like, the preparation process is simple, special hydrophilic modification is not needed, and the process is simplified, the industrial production period is shortened and the production cost is reduced under the condition of meeting the conventional protein elution condition. And meanwhile, the high efficiency of the click reaction can meet the characteristics of high loading capacity, good biocompatibility and the like of the chromatography medium.

The present invention provides a diamond-like carbon film chromatographic column, a preparation method thereof and uses thereof. The preparation method of the diamond-like carbon film chromatographic column is as follows: a ternary nano-composite coating formed by a metal element, an N element and a C element is formed on the inner wall of the chromatographic column by using a micro-arc ion plating technique. The chromatographic column of the present invention can effectively reduce the non-specific adsorption of target analytes sensitive to metals, obtain stable, effective and ideal analysis results, and at the same time does not affect the durability and convenience of the chromatographic column.

The invention relates to a modified luminous particle as well as a preparation method and application thereof. The modified light-emitting particles comprise light-emitting particles and zwitterionic polymers adsorbed on the light-emitting particles, and the modified light-emitting particles can react with active oxygen to generate detectable chemiluminescence signals. According to the scheme provided by the invention, the repulsive force between particles and the non-specific adsorption resistance can be improved, so that the specificity of the light-excited chemiluminescence detection reagent is improved, and the accuracy of a detection result is ensured.

A random terpolymer of N-(2-hydroxypropyl) methacrylamide, carboxybetaine methacrylamide and sulfobetaine methacrylamide, and a polymer brush and to a functionalized polymer brush containing this terpolymer are disclosed. The random terpolymer increases the resistance of the substrate surface to non-specific adsorption of substances from biological media and / or to non-specific interaction with biological media components, and is suitable for use in the form of a polymer brush, for example in sensors or membranes.

The invention is applicable to the technical field of biological detection, and provides a method for detecting staphylococcus aureus based on combination of a screen-printed electrode and CRISPR / Cas13a, a probe containing a DNA / RNA hybrid chain is combined with a magnetic bead to form a simple composite structure, when the Cas13a recognizes 16sRNA of S. aureus, trans-cleavage activity can be immediately activated, the probe chain is rapidly cut off, the cut-off amount depends on the activated amount, and when the Cas13a recognizes 16sRNA of S. aureus, the Cas13a can be used for detecting staphylococcus aureus. And after transient magnetic separation, a result is observed on the screen-printed electrode. The method has ultrahigh specificity: the CRISPR / crRNA sequence ensures that staphylococcus aureus and other microorganisms can be accurately distinguished; the trans-cutting of Cas13a provides chemical amplification, and the magnetic separation purification greatly reduces background signals caused by non-specific adsorption, so that the signal-to-noise ratio and the sensitivity are remarkably improved.

The invention provides a phosphorylated protein enrichment method, and relates to the fields of materials, analytical chemistry and life science. The enrichment method of the phosphorylated protein comprises the following steps: dissolving a phosphorylated protein sample in a sample loading solution, mixing with super-hydrophilic metal ion functionalized microspheres, hatching, centrifuging, and discarding supernatant to obtain a super-hydrophilic material enriched with the phosphorylated protein; mixing the material enriched with the phosphorylated protein with an elution solution, incubating, and centrifuging to obtain enriched liquid of the phosphorylated protein; the super-hydrophilic metal ion functionalized microsphere is provided with a super-hydrophilic nano convex shell which is completely coated on the solid core. The enrichment method provided by the invention can overcome the non-specific adsorption problem caused by modification defects of a traditional monomolecular layer modified functional material, and has excellent enrichment selectivity; the method can overcome the limitation of an immunoprecipitation enrichment method antibody, has wide applicability, and is hopeful to occupy an important position in phosphorylated protein separation and enrichment.

The invention relates to an electrochemical biosensor and application and a detection method and device thereof, zwitterionic DMAPS provides strong antifouling performance for a sensing interface, non-specific adsorption of biomolecules such as protein and cells is effectively prevented, click chemistry is adopted as a sensing strategy, the integrity of a biomolecule structure is maintained, dissociation of an aptamer / CA72-4 is effectively reduced, and the biosensor can be used for detecting the biomolecules. The surface protein binding efficiency is improved, and the biosensor prepared by the method is used for a real patient blood sample and can realize direct and rapid electrochemical detection of CA72-4.

This application discloses a chemiluminescence detection kit for acrylonitrile magnetic microparticles and its detection method. The kit includes a magnetic bead working solution and a luminescent label. The magnetic bead working solution includes acrylonitrile monoclonal antibody A and streptavidin. The luminescent label includes acrylonitrile monoclonal antibody B, modified nano-silica, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N,N-dimethylformamide, acrylonitrile succinimide ester, glutaraldehyde, and ethanolamine. This application uses modified nano-silica as a carrier and grafts a polyethylene glycol hydrophilic layer onto its surface, which amplifies the acrylonitrile ester molecule signal, reduces non-specific adsorption, and improves the sensitivity, specificity, and reliability of the detection.

The invention discloses a nano enzyme with catalyst inactivation resistance and a preparation method and application thereof, and the preparation method comprises the following steps: 1) performing functional pretreatment on DNA to obtain functional modified DNA for subsequent DNA coating construction; and 2) connecting a certain size of metal nanoparticles with catalytic activity with the functionalized modified DNA to construct the DNA coating-coated nano enzyme with catalyst inactivation resistance. The DNA coating on the surface of the prepared nano-enzyme can effectively prevent catalyst inactivation caused by particle aggregation in a high-salt environment, meanwhile, the coating has the function of a molecular sieve, can effectively inhibit non-specific adsorption of biomacromolecules such as protein and the like, allows a small-molecule substrate to be in contact with a catalytic interface, and improves the catalytic activity of the nano-enzyme. Therefore, catalytic activity is maintained in complex biological environments such as serum, sensitive detection of molecules is realized, and a solution is provided for precise medical diagnosis.