291 results about "Non specific adsorption" patented technology

The present teachings provide a manipulation chamber, device, and method related to surface modifiers added to an electrode exposed to the biomolecules or a layer adjacent to an electrode exposed to the biomolecules to decrease non-specific adsorption of the biomolecules such as proteins or nucleic acids in a biological sample.

The invention provides high sensitivity bioassay sensor systems in which non-specific adsorption of impurities such as, for example, proteins, in biological samples is inhibited. Polyethylene glycolated particles enclosing metal or semi-conductor which is in common with the sensor material are used for amplification.

A substrate surface on which either a substance to detect analyte or an analyte per se is immobilized, which surface is formed by a treatment of substrate surface with a liquid which contains uncrosslinked polymer based on polyethylene glycol chain segment, said treatment conducted either simultaneously with the immobilization of said substance or analyte or after said substance or analyte has been immobilized on said surface. The non-specific adsorption of impurity protein or the like which is co-existent in sample for assay is significantly restrained.

The invention belongs to the technical field of inorganic nanometer materials, in particular to a method for preparing shell-core micrometer/nanometer spheres capable of preventing functional materials. The method comprises the step of coating a compact silicon dioxide shell layer on the surfaces of the traditional micrometer/nanometer spheres wrapping functional materials to seal and protect the functional materials with an anti-leakage ratio of 98 percent. The shell layer of silicon dioxide is easy to modify by introducing rich chemical functional groups and convenient to couple biomacromolecules in the field of biomedicines. The shell-core micrometer/nanometer spheres, prepared by the method, have the advantages of avoiding the damage caused by the infiltration of the environmental medium into the micrometer/nanometer spheres to the properties of the functional materials, avoiding the direct contact with the bioactive materials, introducing the rich chemical functional groups to the surfaces of the micrometer/nanometer spheres, and being conveniently crosslinked with biological proteins and nucleic acid molecules, along with smooth surfaces and little non-specific adsorption; and the preparation method has the advantages of simpleness, easy operation, strong operability, good repeatability, and low cost. The shell-core micrometer/nanometer spheres coated with silicon dioxide are stable in performance and long in holding time, and can be widely applied in biochemical analysis fields of biological marking, biological separation, cell imaging and development, biological sensors and the like.

A microfluidic system comprising a microchannel, a carrier fluid comprising a fluorinated oil in the microchannel, at least one plug comprising an aqueous plug-fluid in the microchannel and substantially surrounded on all sides by the carrier-fluid, and a fluorinated surfactant comprising a functional group capable of selectively binding a target molecule is disclosed. A compound for use therewith and a method of synthesizing a fluorinated surfactant are also provided.

The invention discloses a preparation method of a graphene oxide/alpha fetoprotein aptamer electrochemical sensor. The preparation method comprises the following steps: taking carboxylated graphene oxide as a substrate, grafting an aminated alpha fetoprotein aptamer chain on the substrate through carbodiimide reaction, then modifying the obtained compound on an electrode, closing a non-specific adsorption site on the modified electrode with bovine serum albumin, so that a graphene oxide/alpha fetoprotein aptamer electrochemical sensing interface is formed; and taking the modified electrode as a working electrode, and investigating variation, caused by alpha fetoprotein solution with different concentrations, of an impedance signal on the sensing interface by adopting an impedance method. Compared with an existing alpha fetoprotein detection method, the preparation method has the advantages that an alpha fetoprotein aptamer is adopted for analyzing a target protein, harms such as redundant multiple labeling operation, radiation and pollution do not exist, and the sensor has the advantages of high sensitivity, good selectivity, simplicity in preparation, high analysis speed, mild reaction conditions, low preparation cost and the like.

The invention relates to a bioactive polysaccharide self-assembly finishing polyurethane material and a preparation method thereof. The bioactive polysaccharide self-assembly finishing polyurethane material is characterized by comprising a substrate and a finishing layer, wherein the substrate is made of a polyurethane material, and the finishing layer is prepared by finishing the surface of the substrate by using a layer-by-layer self-assembly technique; and the finishing layer contains electronegative lentinan sulfate and electropositive chitosan which are alternately self-assembled layer by layer on the surface of the substrate. The surface of the polyurethane material has favorable hydrophilicity, favorable function of resisting fibrinogen non-specific adsorption, high antibacterial activity for inhibiting Bcillus pyocyaneus and other bacteria, and favorable cell compatibility. In addition, the polyurethane material has the advantages of simple preparation technology, easy control, mild preparation conditions and low cost, and is especially suitable for preparing biomedical materials for artificial organs and devices with complicated shape and structure.

A polystyrene microsphere is provided. The polystyrene microsphere is micron or nano-scale, and the surface of the microsphere is coated with a polyacrylic resin Eudragit. The polystyrene microsphereof the present invention is a polystyrene microsphere with very low non-specific adsorption, and the surface of the microsphere contains a functional group carboxyl group, so that further functional modification of the surface of the polystyrene microsphere is not required, the operation is further greatly simplified, and the loss is reduced.

The present invention relates to a method for improving the efficiency of biochemical reactions in channels of microfluidic devices. More specifically, the present invention relates to the use of chitosan or a chitosan derivative for coating channel surfaces to reduce non-specific adsorption of reagents to microfluidic channels. This reduction of non-specific adsorption improves the efficiency and reproducibility of the reaction, e.g., amplification reactions, such as PCR, and reduces cross-contamination.

The invention discloses a kit and a method for detecting hepatitis B virus surface antigen (HBsAg). The detection kit is used for marking hepatitis B virus surface antibody (HBsAb) by using metalloporphyrin obtained by using water-soluble A3B type metalloporphyrin to mark the HBsAb. The detection kit and the detection method have the advantages that: (1) free metalloporphyrin molecules are separated from a coupled substance easily after an A3B type metalloporphyrin complex is coupled with the HBsAb to obtain a high-purity labelled antibody, so that non-specific adsorption background signals are reduced when the HBsAg is detected by chemical luminescence immunodetection, and detection sensitivity is greatly improved; (2) the method is simple, convenient and quick; automation is realized easily; and specificity of immunoreaction, high sensitivity of chemical luminescence reaction and stability of a chemical marker are achieved; and (3) a new method is provided for detecting the HBsAg quickly and accurately, and preventing and treating the hepatitis B virus.

The invention provides a method for detecting specific and/or non-specific adsorption of nucleic acids. The method comprises the following steps: reacting a to-be-detected nucleic acid with a first probe, wherein the first probe is immobilized on the surface of a first substrate, and the to-be-detected nucleic acid and the first probe are at least partially complementary and have different labels,and the labels can generate signals; detecting signals on the surface of the first substrate to obtain first detection results; reacting the to-be-detected nucleic acid with a second probe, wherein the second probe is immobilized on the surface of a second substrate, the second probe has second labels, and the to-be-detected nucleic acid is not complementary to the second probe; detecting signalson the surface of the second substrate to obtain second detection results; and based on the first detection results and the second detection results, detecting the specific and/or non-specific adsorption of the to-be-detected nucleic acid. The method can detect the specific and/or non-specific adsorption of the nucleic acid and the surfaces of the substrates or the probes on the surfaces of the substrates, and is applicable to production quality control of chips.

A method for producing a non-specific adsorption inhibitor includes reacting (A) a tosylated compound of polyoxyethylene monomethyl ether with (B) a polyamine having either an amino group or imino group (—NH—), or both, in total of 3 to 12.

The invention relates to a pH sensitive type liver-targeted compound nano drug delivery system based on sodium alginate and a preparation method. The system is prepared by blending a liver-targeted carrier GA-ALG-mPEG or GA-ALG and a pH sensitive type drug carrier DOX-ALG-mPEG or DOX-ALG proportionally in a mode of dialysis through self assembly. The preparation of the material comprises a sodium alginate derivative (ALG-mPEG), the liver-targeted carrier and the pH sensitive type drug carrier. By the modification of the hydrophilic mPEG according to the compound nano drug delivery system, the non-specific adsorption with cells and protein can be effectively avoided. Meanwhile, the capabilities of drug loading and ligand targeting of the material are enhanced. The pH sensitive type liver-targeted compound nano drug delivery system based on the sodium alginate has the function of liver targeting. Meanwhile, the drug release of a fixed point at the part of a tumor can also be realized according to the difference of pH at normal tissues and the part of the tumor so as to enhance the curative effect and lower the side effect of drugs. The preparation method is simple, is easy to amplified production and has favorable application prospect.

A binding protein molecule, characterized in that it has a first domain having a binding site to an inhibitor of non-specific adsorption in which the domain comprises a part of the variable region of an antibody as the binding site and a second domain having a binding site to a target substance in which the domain comprises a part of the variable region of an antibody as the binding site, wherein the first and second domains are bound via a linker.

The invention discloses a laminated crossed fluorescent amplified magnetic separating and detecting method of DNA, which comprises the following steps: marking DNA of two different sequences a and b on the SiO2 nanometer particle simultaneously; marking DNA on the magnetic nanometer particle surface of sequence e; crossing one end of target DNA and the DNA of the sequence b on the SiO2 nanometer particle; crossing the other end of target DNA and DNA of sequence e on the magnetic nanometer particle; marking the DNA of two different sequences c and d on the SiO2 nanometer particle simultaneously; crossing DNA of SiO2 nanometer particle on the sequence c and fluorescent DNA; finishing the first sandwich crossing; crossing the DNA of first sandwich crossed SiO2 nanometer particle on the sequence a and one end of connected DNA; crossing the other end of DNA and the DNA on the nanometer particle on the sequence d after fluorescent cross; cleaning the product after the second sandwich cross through magnetic separation; removing residual fluorescent molecule and non-specific adsorption; denaturizing the DNA after the second sandwich cross; proceeding fluorescent detection for the unlinked fluorescent DNA.

The invention relates to a highly cross-linked monodisperse epoxy-group-containing polymer microsphere and a preparation method thereof. The microsphere contains an epoxy group, methacrylic acid glycidyl ester is used as a monomer, divinyl benzene (DVB) is used as a cross-linking agent, and the microsphere is obtained through polymerization; the particle size of the microsphere is 2 to 10 [mu]m, and the polydispersity index is 1.04 to 1.07. The preparation method comprises the following steps: (1), methacrylic acid glycidyl ester, the DVB and an initiating agent are together added into a solvent for uniform mixing, after nitrogen is introduced, sealing is performed, the reaction temperature is controlled to be 82 to 90 DEG C, and the reaction time lasts for 3 to 5 hours; (2), a reaction product obtained in the step (1) is washed and dried, so that the polymer microsphere is obtained. The polymer microsphere prepared by the invention contains the epoxy group, the epoxy group is an active group, derivative reaction is facilitated, and the problems of high hydrophobicity, high probability of non-specific adsorption can be solved.

An object of the present invention is to provide a labeled particle having a high reactivity with an antigen and a suppressed non-specific adsorption, and an immunochromatographic method using the labeled particle. The present invention provides a labeled particle, wherein a fragmented antibody is immobilized to a labeling substance via a chemical bond.

The invention discloses a method for detecting multiple targets based on nucleic acid aptamer fluorescence sensor, which applies polypyrrole nanoparticles and DNA silver nanoclusters for the first time and establishes an aptamer sensing platform based on a steric hindrance effect.In addition, the invention obtains a good detection result based on the low non-specific adsorption characteristic ofthe polypyrrole nanoparticles, and realizes the detection of multiple targets based on the specific recognition characteristic of the aptamer sequence, improving the universal performance of the invention.The method for detecting multiple targets based on nucleic acid aptamer fluorescence sensor, provided by the invention, has the advantages of label-free property, no enzyme and high sensitivity,and can realize high-efficiency detection in biological samples.

The present invention relates to surface-modified substrates that demonstrate reduced non-specific adsorption of biological agents. The substrates are silicon or carbon substrates having ethylene glycol oligomers covalently bound to at least one substrate surface. The substrates may be used in sensor devices, such as biochips, and in implantable medical devices in order to reduce the non-specific binding of biological agents.

The invention relates to a temperature response type beta-cyclodextrin silica gel stationary phase. The structural formula of the temperature response type beta-cyclodextrin silica gel stationary phase is as shown in the specification. The preparation method comprises the steps of deriving beta-cyclodextrin to obtain a beta-cyclodextrin derivative with a reaction group and a functional group, then performing silylation reaction, modifying a silica gel surface with a silylation reagent with two bonds, performing polymerization reaction on acrylamide and a glycidyl ether monomer to form a meshed temperature-sensitive polymer layer on the silica gel surface through azodiisobutyronitrile serving as an initiator, and finally implanting the beta-cyclodextrin derivative into a polymer chain to obtain the temperature response type beta-cyclodextrin silica gel stationary phase. The preparation process is simple, and the property is stable; the prepared temperature response type beta-cyclodextrin silica gel stationary phase can overcome the shortcomings that silica gel cannot resist water and alkali and undergoes non-specific adsorption; a prepared separation material is novel in structure and flexible in application; effective separation of compounds can be realized by controlling the column temperature; furthermore, the beta-cyclodextrin derivative in the polymer chain can improve the hydrophilicity and the chiral recognition capacity of the stationary phase, and is suitable for separation and chiral separation of polar and hydrophilic compounds and chemical molecules.

A non-specific adsorption inhibitor includes a water-soluble polymer obtained by polymerizing a monomer unit that includes a monomer (A) that forms a polymer that has a lower critical solution temperature (LCST) of 80° C. or less in an aqueous solution under normal pressure when subjected to homopolymerization, and a monomer (B) that forms a polymer that does not have the LCST in an aqueous solution under normal pressure when subjected to homopolymerization.