35results about How to "Inhibition of non-specific adsorption" patented technology

The invention discloses a three-dimensional carrier of a microarray biochip and a preparation method thereof. The three-dimensional carrier of a microarray biochip is prepared by growing a zinc oxide nanorod on the surface of a substrate material and growing a polymer brush on the surface of the zinc oxide nanorod. The preparation method comprises the following steps of: activating the surface of the substrate material, and growing a zinc oxide nanorod on the surface of the substrate material; performing surface modification on the grown zinc oxide nanorod; connecting the modified surface of the zinc oxide nanorod with an initiator; and finally, growing a polymer brush to obtain a three-dimensional carrier of a microarray biochip. The preparation method is simple, does not need special equipment, has low production cost and good repeatability, and can be used for realizing large-scale production; the prepared three-dimensional carrier has low price and high fixing density for probe molecule, can be used for effectively inhibiting non-specific protein adsorption and improving the sensitivity and the specificity, and can be widely applied to the fields of biology, basic medicine, disease diagnosis, drug screening, food safety, environmental monitoring and the like.

The present invention relates to a bilayer lipids membrane surface modified protein chip, a silicon substrate being a solid substrate on which being, in turn, a L-alpha-phosphatidylethanolamine monomolecular layer linked to the silicon substrate through covalent bonds and a p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer associated with the phosphatidylethanolamine monomolecular layer by a hydrophobic effect. The protein chip is obtained by fixing the L-alpha-phosphatidylethanolamine monomolecular layer at the surface of the silicon substrate through covalent bonds in a micro- runner system and then adsorbing the p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer. Said protein chip is capable of applied in an imaging ellipsometry biosensor to explore target molecules capable of associating specifically therewith by covalently fixing ligands molecules at terminal tetrafunctional radicals of the protein chip surface PEG, wherein the surface morphology changes of the protein chip are capable of being observed in said imaging ellipsometry system, after both are associated. The bilayer lipids membrane surface modified protein chip in accordance with the present invention is capable of avoiding effectively a nonspecific adsorption, with its ligands molecules having excellent stability and being kept high biological activities.

The invention discloses a method for preparing a dendritic-polymer-based microarray antibody chip and a product of the method. The method for preparing the dendritic-polymer-based microarray antibody chip comprises the steps that a glass slide is cleaned in ethyl alcohol, and then is immersed in an ethanol solution containing 3-glycidoxypropyltrimethoxysilane, and after annealing, the glass slide is immersed into a dry dichloromethane or tetrahydrofuran solution containing triethylamine and alpha-bromoisobutyryl bromide, and then is flushed with dichloromethane or tetrahydrofuran; nitrogen is blown into a methanol solution containing oligo (ethylene glycol) methacrylate and glycidyl methacrylate, 2,2'-bipyridyl and cuprous bromide are added into the methanol solution under ultrasonic waves, the glass slide is immersed into the methanol solution, and the methanol solution is sealed and stored in an inert and light-avoiding atmosphere box to obtain an activated carrier material; finally, a microarray chip sample application instrument is used for printing detection antibodies on the activated carrier material, standing is carried out, and the microarray antibody chip is obtained through washing with a phosphate buffer solution. The obtained microarray antibody chip is high in sensitivity, good in specificity and capable of being used for early stage screening of diseases.

The invention discloses a biological detector based on the interference effect of an ordered porous nanostructure film. The biological detector includes a detection pool, a sensing unit arranged in the detection pool, and an optical fiber spectrometer for detecting the reflection interference spectrum of the sensing unit. The sensing unit is a porous film with three-dimensional ordered nanopores, and the diameter of the nanopores is 20nm-500nm. The invention also discloses a method for detecting biomolecules by using the biodetector. When the substance to be measured is adsorbed on the pore surface of the ordered porous film as the sensing unit, it will change the spectral distribution of the reflected interference light, resulting in a spectral line shift, which can be used to quantitatively or qualitatively analyze the sample The concentration of substances in the medium, the attachment rate, the interaction, the change of the geometric size, etc.

The invention discloses a synthesizing method of phosphocholine chitosan derivant, which comprises the following steps: reacting chitose powder and disubstituted Choline phosphonate with different proportions in the composite system of alkaline, carbon tetrachloride and secondary alcohol at 0-40 Deg C for 12-24h; hydrolyzing through alkaline liquid to obtain the product with different coupling rate. The invention modifies biological function and compatibility, which can be applied in the drug and gene carrier, tissue engineering rack, biological medical film, aquagel and biological intelligent instrument domain.