Superposition type fixed bed phase-conversion focusing chromatographic column and separation method

A technology of focused chromatography and fixed bed, which is applied in superimposed fixed bed phase-disguised focusing chromatography column, superoxide dismutase and compound amino acid, and separates lumbrokinase at the same time, achieving the effect of improving work efficiency and shortening the separation time.

Inactive Publication Date: 2014-02-12
北京双螺旋生物技术研究所
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Problems solved by technology

[0009] In order to solve the problems existing in the above-mentioned prior art, the object of the present invention is to provide a superimposed fixed bed phase-changing focusing chromatography column and a separation method, which can change the status quo of a single finished product in the current bioslurry production line, while maintaining the quality of the original chemical product Under the premise of the original level of economic accounting indicators such as production, energy consumption, and working hours, the intensive and continuous separation of various medicinal components in the same column bed is realized.

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  • Superposition type fixed bed phase-conversion focusing chromatographic column and separation method
  • Superposition type fixed bed phase-conversion focusing chromatographic column and separation method
  • Superposition type fixed bed phase-conversion focusing chromatographic column and separation method

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[0041] Related terminology

[0042] The double meaning of "disguised phase", one refers to the phenomenon of sequential classification and aggregation of various target substances on the column bed, which is similar to isoelectric focusing, but this technology does not rely on the participation of gradient media at all, and this "sequential classification and aggregation" without the concept of gradient only It can be called "disguise" to show the difference. In addition, it means that the slurry needs to pass through different separation areas during the separation process. Every time it reaches a pre-set area, it needs to leave the corresponding target substance, and then carry other solutes to be separated into the next pre-set area. This requires necessary adjustments to the physical and chemical parameters of the mobile phase before entering the next column section, in order to meet the requirements of the optimal separation conditions for the next target substance. This ad...

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Abstract

The invention provides a superposition type fixed bed phase-conversion focusing chromatographic column and further provides a method which simultaneously separates lumbrukinase, superoxide dismutase and compound amino acid by adopting the same. The present status that single finished products are produced in the traditional biological pulp production line is reformed, and intensified continuous separation for a plurality of drug effect ingredients in the same column bed is realized under the premise that the economic accounting indicators, such as quality, yield, energy consumption, working hours and the like of the original biochemical products are kept at the original level.

Description

technical field [0001] The invention relates to a superimposed fixed bed phase-changing focusing chromatographic column, and also relates to a method for simultaneously separating lumbrokinase, superoxide dismutase and compound amino acids by using the chromatographic column, belonging to the fields of biochemical separation media and biochemical pharmacy. Background technique [0002] When modern biochemical technology extracts biological products on a large scale, it generally follows the principle of a single target substance, that is, to specifically separate a single substance. For example, the earthworm body is a treasure trove of rich natural medicine resources. Lumbrokinase EK, superoxide dismutase SOD, and compound amino acid AA are three medicinal ingredients in the earthworm body that are beneficial to the human body. In order to realize their large-scale production requirements, they usually need to be designed separately. Three technological processes, construct...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/08C12N9/48C12N9/08C07C227/40
Inventor 倪宝谦倪成
Owner 北京双螺旋生物技术研究所
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