Method for modifing protein chip surface

Abstract

The invention discloses a method to modify proteid chip surface, its character is fixing a layer of low molecular weight proteid molecule on the chip by normal method at first, then fixing the proteid chip which is needed to be fixed. The low molecular weight is 1X10 to the power 3 -9X10 to the power 5 Dalton. The method can modify the surface character of the proteid chip surface: on one hand, because of the covering of the low molecular weight molecule on the chip surface, the biology activity of the unfixed proteid molecule can be ensured, and at the same time, the aspecfic absorb is controlled on the surface; on the other hand, the low molecular weight molecule has amino, carboxyl, hydroxyl and other active group polar aminophenol on the proteid molecule surface, they can be used to fix porteid molecule directly after being activeted, and to form stable covalent bond.

Description

technical field

[0001] The invention relates to a method for modifying the surface of a protein chip. Background technique

[0002] With the development of science and technology, protein chips have been widely used in many biomedical fields. Commonly used protein chips are to immobilize target protein molecules on the surface of a solid substrate. Protein is a kind of organic macromolecule with complex composition and structure, which can interact with and adsorb on almost all solid surfaces. In the fabrication of protein chips, it is not only necessary to stably immobilize protein molecules on the surface to maintain their biological activity, but also to be able to effectively inhibit non-specific adsorption on the surface.

[0003] At present, the methods of immobilizing proteins on solid surfaces are mainly through physical adsorption or covalent linkage. As described in Jonsson, U.; Ivarsson, B.; Lundstrom, I.; Berghem, L.J. Colloid. Interactions such as bonds secu...