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Liquid chromatography column, and method for analyzing hemoglobin

A technology of liquid chromatography and analysis method, applied in the field of columns for liquid chromatography, can solve problems such as high price of engineering plastics, and achieve the effects of inhibiting non-specific adsorption, high separation performance, and inexpensive production

Active Publication Date: 2013-12-11
SEKISUI MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, engineering plastics represented by PEEK have the disadvantage of being expensive

Method used

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  • Liquid chromatography column, and method for analyzing hemoglobin
  • Liquid chromatography column, and method for analyzing hemoglobin
  • Liquid chromatography column, and method for analyzing hemoglobin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、2 and comparative example 1～5

[0059] Empty column preparation

[0060] The materials shown in Table 1 were used for the column tube and end fittings, and a column with an inner diameter of 4.6 mm and a length of 15 mm was prepared.

[0061] In Comparative Example 1, the stainless steel (SUS316) column was coated with silicone resin using SR2410 (manufactured by Dow Corning).

[0062] Table 1

[0063]

Column material

Example 1

polyethylene

Example 2

Polymethylmethacrylate

Comparative example 1

Stainless steel (SUS316) is coated with silicone resin

Comparative example 2

Stainless steel (SUS316) without surface treatment

Comparative example 3

Polypropylene

Comparative example 4

polyethylene terephthalate

Comparative Example 5

polycarbonate

[0064] Preparation of Filler Particles

[0065] To 2000 mL of an aqueous solution of 3% by weight polyvinyl alcohol (manufactured by Nippon Synthetic Chemical Industry Co...

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PUM

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Abstract

A purpose of the present invention is to provide a liquid chromatography column which can be produced at a low cost and rarely undergoes the non-specific adsorption of a biological component thereon. In addition, the purpose of the invention is to provide a method for analyzing hemoglobin by using the liquid chromatography column. The present invention is a liquid chromatography column comprising a column pipe and an end fitting, wherein the column pipe and the end fitting are made of polyethylene or polymethyl methacrylate.

Description

technical field [0001] The present invention relates to a column for liquid chromatography. Furthermore, the present invention relates to a method for analyzing hemoglobins using the column for liquid chromatography. Background technique [0002] In the fields of organic chemistry, biochemistry, medicine, etc., liquid chromatography is widely used in the determination or analysis of components in samples. For example, in the medical field, liquid chromatography is used to measure hemoglobin A1c, which is a diagnostic index for diabetes. Hemoglobin A1c is glycated hemoglobin in which sugar in the blood is chemically bonded to the N-terminus of the β chain of hemoglobin. It can be said that the ratio of hemoglobin A1c in hemoglobins, that is, the ratio of glycated hemoglobin to the total of glycated hemoglobin and non-glycated hemoglobin reflects 1 to 2 Blood glucose averages over a period of months. The hemoglobin A1c value (%), which represents the ratio of hemoglobin A1c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/60G01N30/88
CPCG01N30/60G01N30/88G01N2030/8831G01N30/6004G01N2030/8822G01N30/6052
Inventor 与谷卓也
Owner SEKISUI MEDICAL CO LTD
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