Method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using ion complementary peptide

A dimethylsiloxane, hydrophobic coating technology, applied in coatings, chemical instruments and methods, laboratory containers, etc., can solve the problems of low anti-protein adsorption performance, unstable coatings, etc. Enhanced protein adsorption performance, enhanced coating stability, and inhibition of non-specific adsorption
CN104497334AInactive Publication Date: 2015-04-08SHAANXI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHAANXI NORMAL UNIV
Publication Date
2015-04-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using an ion complementary peptide, which comprises the following steps: by using an ion complementary peptide EAR16-II or ion complementary peptide EAR16-II, methyl cellulose and dodecyl-beta-D-maltoside as a surface modifier, carrying out physical adsorption to perform self-assembly on the polydimethylsiloxane microchannel surface so as to form a stable hydrophobic film; and in the presence of saccharide derivatives, changing the configuration of the EAR16-II to form the more stable beta-folding self-assembled hydrophobic film. The method can effectively inhibit the non-specific adsorption of the protein to the microchannel surface, enhances the surface anti-pollution capacity and biological compatibility of the polydimethylsiloxane, and implements high-efficiency recurrent separation of the analyte.
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Description

technical field

[0001] The invention belongs to the technical field of microfluidic chip separation, and in particular relates to a method for modifying the surface of a polydimethylsiloxane microfluidic chip to realize the antifouling property of the surface. Background technique

[0002] Polydimethylsiloxane (PDMS) is a widely used material for making microfluidic chips. It has the advantages of non-toxicity, good air permeability, good chemical inertness and optical properties, but the surface of PDMS is highly hydrophobic, The electroosmotic flow is unstable, and the adsorption of analytes, especially proteins, is serious. The most effective way to overcome the shortcomings of PDMS is to modify the chip surface.

[0003] At present, the commonly used surface modification methods mainly include physical adsorption coating method and chemical bonding coating method. The chemical bonding coating method is a method with cumbersome steps, and PDMS itself is chemically inert...

Claims

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