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Method for preparing shell-core micrometer/nanometer spheres capable of preventing functional materials

A technology of functional substances and nanospheres is applied in the field of preparation of core-shell micro/nanospheres, which can solve the problems of easily damaged active substance activity, difficult biomacromolecule coupling, limited biomedical applications, etc. The preparation method is simple and easy to operate, and the operability is strong

Inactive Publication Date: 2010-06-09
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functional substances of the functional micro / nanospheres prepared by this method are easy to detach from the polymer matrix, leak, have short storage time, and unstable product quality.
Moreover, the surface of the polymer becomes rough after swelling; it has high non-specific adsorption to biomacromolecules; the functional substances exposed on the surface of the polymer are in direct contact with the biologically active substances, which is easy to damage the activity of the active substances
In addition, some commonly used polymer micro / nanospheres (such as polystyrene micro / nanospheres) have poor chemical functional groups on the surface and are not easy to couple with biological macromolecules, which limits their biomedical applications.

Method used

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  • Method for preparing shell-core micrometer/nanometer spheres capable of preventing functional materials
  • Method for preparing shell-core micrometer/nanometer spheres capable of preventing functional materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033](1) Prepare a mixed solution of deionized water and isopropanol with a volume ratio of 1:5, and add 100 mg of fluorescent polystyrene microspheres (12 μm) loaded with CdSe / ZnS quantum dots to the above 100 mL mixed solution; (2 ) Weigh 10mg of polyvinylpyrrolidone (PVP) into the above system, stir to dissolve and disperse for 60min; (3) Add 1mL tetraethyl orthosilicate (TEOS) to the above system at room temperature, and stir for 30min; (4) Add 1mL Ammonia water was added to the above system, and the reaction was closed with stirring for 24 hours. The obtained product is centrifuged at high speed, washed with water, and the obtained product can be maintained for a long time in PBS buffer solution. It can be seen from the figure that the quantum dot fluorescent microspheres with the core-shell structure have good dispersion and high fluorescence intensity (calculated, after the core-shell structure wraps, the fluorescence efficiency of the quantum dot fluorescent microsphe...

Embodiment 2

[0035] (1) Prepare a mixed solution of deionized water and isopropanol with a volume ratio of 1:3, and 10 mg of Fe 3 o 4 Magnetic polystyrene microspheres (12μm) were added to the above 10mL mixed solution; (2) 1mg of polyvinylpyrrolidone (PVP) was weighed and added to the above system, and stirred to dissolve and disperse for 10min; (3) At room temperature, 0.01mL of normal Ethyl silicate (TEOS) was added to the above system, and stirred for 30 minutes; (4) 0.01 mL of ammonia water was added to the above system, and stirred for 24 hours to seal the reaction. The obtained product is centrifuged at high speed, washed with water, and the obtained product can be kept in deionized water for a long time. It can be seen from the figure that the ferroferric oxide magnetic microspheres with a core-shell structure have good dispersion, and a single sphere is black, and black ferric oxide nanoparticles are successfully wrapped in the microspheres.

Embodiment 3

[0037] (1) Prepare a mixed solution of deionized water and isopropanol with a volume ratio of 1:7, add 50 mg of Cy5-loaded fluorescent polyacrylamide microspheres (3 μm) to the above 50 mL mixed solution; (2) weigh 6 mg Add polyvinylpyrrolidone (PVP) to the above system, stir to dissolve and disperse for 30 minutes; (3) add 0.5mL tetraethyl orthosilicate (TEOS) to the above system at room temperature, and stir for 30 minutes; (4) Add 0.5mL ammonia water to To the above-mentioned system, stir and close the reaction for 24h. The obtained product is centrifuged at high speed, washed with water, and the obtained product can be kept in ethanol for a long time.

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Abstract

The invention belongs to the technical field of inorganic nanometer materials, in particular to a method for preparing shell-core micrometer / nanometer spheres capable of preventing functional materials. The method comprises the step of coating a compact silicon dioxide shell layer on the surfaces of the traditional micrometer / nanometer spheres wrapping functional materials to seal and protect the functional materials with an anti-leakage ratio of 98 percent. The shell layer of silicon dioxide is easy to modify by introducing rich chemical functional groups and convenient to couple biomacromolecules in the field of biomedicines. The shell-core micrometer / nanometer spheres, prepared by the method, have the advantages of avoiding the damage caused by the infiltration of the environmental medium into the micrometer / nanometer spheres to the properties of the functional materials, avoiding the direct contact with the bioactive materials, introducing the rich chemical functional groups to the surfaces of the micrometer / nanometer spheres, and being conveniently crosslinked with biological proteins and nucleic acid molecules, along with smooth surfaces and little non-specific adsorption; and the preparation method has the advantages of simpleness, easy operation, strong operability, good repeatability, and low cost. The shell-core micrometer / nanometer spheres coated with silicon dioxide are stable in performance and long in holding time, and can be widely applied in biochemical analysis fields of biological marking, biological separation, cell imaging and development, biological sensors and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic nanometer materials, and in particular relates to a method for preparing core-shell micro / nanospheres capable of preventing leakage of functional substances. Background technique [0002] The application of functional micro / nanospheres in the field of biomedicine has attracted more and more attention. The most representative functional micro / nanospheres are magnetic micro / nanospheres and fluorescent micro / nanospheres. They are the hotspots of scholars at home and abroad. At present, there are commercially available magnetic micro / nanospheres and fluorescent micro / nanospheres. Most of its preparation methods adopt the embedding method, that is, by swelling the polymer carrier, the functional substances mixed with it (such as magnetic nanoparticles, fluorescent dye molecules, quantum dots, etc.) penetrate into the polymer matrix to achieve embedding. Combined with the role of fixed functional s...

Claims

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Application Information

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IPC IPC(8): B01J13/02
Inventor 张兵波王祎龙赵鹏郭方方时东陆
Owner TONGJI UNIV
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