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Preparation method and application of magnetic microsphere for biological protein separation

A magnetic microsphere and biological protein technology, applied in the field of biological protein separation, can solve the problems that the specific separation effect of biological protein needs to be further studied, and the preparation process of magnetic microspheres is complicated.

Active Publication Date: 2014-09-10
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of the magnetic microspheres disclosed in this paper is relatively complicated, and its specific separation effect on biological proteins needs to be further studied

Method used

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  • Preparation method and application of magnetic microsphere for biological protein separation
  • Preparation method and application of magnetic microsphere for biological protein separation
  • Preparation method and application of magnetic microsphere for biological protein separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Preparation of magnetic microsphere matrix

[0031] The magnetic microsphere matrix with carboxyl functional groups used in this example was prepared according to Example 1 in Chinese Patent CN92105584.

[0032] 2) Preparation of emulsion

[0033] The components of the emulsion and their mass percentages are as follows:

[0034]

[0035] The above-mentioned components are mixed in proportion, ultrasonically treated to dissolve all the solid components, and then further fully mixed.

[0036] 3) Preparation of dispersion system

[0037] The magnetic microsphere matrix obtained in step 1) is dispersed in the emulsion prepared in step 2) at a concentration of 20 mg / mL, and the magnetic microsphere matrix is ​​uniformly dispersed by ultrasonic treatment to obtain a dispersion system.

[0038] 4) Polymerization and post-treatment

[0039] The dispersion system prepared in step 3) was stirred and reacted at 75° C. for 30 hours, the supernatant was separated by a magn...

Embodiment 2

[0067] 1) Preparation of magnetic microsphere matrix

[0068] The magnetic microsphere matrix used in this example was prepared according to Examples 1-6 in Chinese Patent Publication CN102746529A. That is, polystyrene polymer seed particles are first prepared; then the seed particles are modified with an emulsion containing polyvinylpyrrolidone, divinylbenzene, styrene and toluene (pore forming agent), and the porosity is obtained by emulsion polymerization. polystyrene particles; the porous polystyrene particles were nitrated using nitric acid; then FeSO 4 Iron was incorporated into the porous polystyrene particles; and then the resulting particles were coated and carboxyl functionalized to obtain the magnetic microsphere matrix with carboxyl functional groups used in this example.

[0069] 2) Preparation of emulsion

[0070] The components of the emulsion and their mass percentages are as follows:

[0071]

[0072] The above-mentioned components are mixed in proportio...

Embodiment 3

[0082] Experiment with reference to the steps of Example 2, the difference is only to replace the emulsion in Example 2 with the following emulsion:

[0083] The components of the emulsion and their mass percentages are as follows:

[0084]

[0085]

[0086] The results are shown in Table 5-8.

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Abstract

The invention provides a preparation method of a magnetic microsphere for biological protein separation, which comprises the following steps: preparing and using a suitable emulsion to treat a magnetic microsphere matrix, and performing emulsion polymerization to modify the surface of the magnetic microsphere matrix, thus obtaining the magnetic microsphere of which the surface is coated with a polyacrylate polymer layer, wherein the emulsion comprises the following components: a monoacrylate compound, a diol acrylate compound, an initiator, and optional anionic surfactant and water. When being used for biological protein separation, the magnetic microsphere obviously reduces the non-specific adsorption of other proteins on the premise of not influencing the capability of linking to a specific protein, thus providing a new choice for separation engineering of high-protein-specificity adsorption.

Description

technical field [0001] The invention relates to the technical field of biological protein separation, in particular to a preparation method and application of magnetic microspheres for biological protein separation. Background technique [0002] Magnetic nanoparticles (such as Fe 3 o 4 Nanoparticles) not only have the unique properties of nanomaterials, such as small particle size, large specific surface, high coupling capacity, etc., but also have magnetic responsiveness and superparamagnetism, which can be aggregated and positioned under a constant magnetic field, and can be assembled and positioned under an alternating magnetic field. Absorb electromagnetic waves to generate heat. In addition, magnetic nanoparticles can also be modified with various active functional groups (such as -OH, -COOH, -NH3, etc.) through surface modification. Therefore, magnetic nanoparticles have broad application prospects in biomedical fields such as bioseparation, drug release, and hyperth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/14C08F222/14C08F220/28C08F220/18C08F2/26C08F2/44C07K1/22G01N33/53G01N33/577
Inventor 饶微杜凯赵莉
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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