Magnetic nanoparticle and immunomagnetic nanoparticle with cell-like structure as well as preparation method and application thereof

A magnetic nanoparticle and cell-mimicking technology, applied in the field of biomimetic immunomagnetic nanomaterials, can solve the problems of complex process and long cycle, and achieve the effect of good biocompatibility and good magnetic response performance.

Inactive Publication Date: 2019-03-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In short, the traditional extraction of cell membranes and the integration of cell membranes and magnetic nano-platforms involve complex processes, long cycles, and the need for special extruders and other equipment

Method used

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  • Magnetic nanoparticle and immunomagnetic nanoparticle with cell-like structure as well as preparation method and application thereof
  • Magnetic nanoparticle and immunomagnetic nanoparticle with cell-like structure as well as preparation method and application thereof
  • Magnetic nanoparticle and immunomagnetic nanoparticle with cell-like structure as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] In this embodiment, the preparation of immunomagnetic nanoparticles with a cell-like structure includes the following steps:

[0056] (1) Preparation of graphene and PEI coated Fe 3 o 4 Magnetic nanoparticles (denoted as product L1, MNs@PEI@G), including the following sub-steps:

[0057] (11) Fe 3 o 4 Magnetic nanoparticles (MNs) were uniformly dispersed in deionized water to obtain Fe 3 o 4 The concentration of magnetic nanoparticles is 1mg / mL suspension, polyethyleneimine (PEI) is dissolved in deionized water to obtain a PEI aqueous solution with a concentration of 1.5mg / mL; 5mL is uniformly dispersed with Fe 3 o 4 After mixing the suspension of magnetic nanoparticles and 0.5mL PEI aqueous solution, react under shaking conditions for 2h, carry out magnetic separation on the obtained reaction solution and collect the separated solid product, and then wash the solid product three times with deionized water to obtain PEI coated Fe 3 o 4 Magnetic nanoparticles (M...

Embodiment 2

[0080] In this embodiment, the preparation of immunomagnetic nanoparticles with a cell-like structure includes the following steps:

[0081] (1) Preparation of graphene and PAH coated Fe 3 o 4 Magnetic nanoparticles (denoted as product L1, MNs@PAH@G), including the following sub-steps:

[0082] (11) Fe 3 o 4 Magnetic nanoparticles (MNs) were uniformly dispersed in deionized water to obtain Fe 3 o 4 The concentration of magnetic nanoparticles is 1.25mg / mL suspension, polyallylamine hydrochloride (PAH) is dissolved in deionized water to obtain a PEI aqueous solution with a concentration of 1mg / mL; 5mL is uniformly dispersed with Fe 3 o 4 After mixing the suspension of magnetic nanoparticles and 0.5mL PAH aqueous solution, react under shaking conditions for 1h, carry out magnetic separation on the obtained reaction solution and collect the separated solid product, and then wash the solid product three times with deionized water to obtain PAH coated Fe 3 o 4 Magnetic nano...

Embodiment 3

[0093] In this embodiment, the preparation of immunomagnetic nanoparticles with a cell-like structure includes the following steps:

[0094] (1) Preparation of graphene and PEI coated Fe 3 o 4 Magnetic nanoparticles (denoted as product L1, MNs@PEI@G), including the following sub-steps:

[0095] (11) Fe 3 o 4 Magnetic nanoparticles (MNs) were uniformly dispersed in deionized water to obtain Fe 3 o 4 The concentration of magnetic nanoparticles is 1.25mg / mL suspension, polyethyleneimine (PEI) is dissolved in deionized water to obtain a PEI aqueous solution with a concentration of 2mg / mL; 2mL is uniformly dispersed with Fe 3 o 4 After mixing the suspension of magnetic nanoparticles and 0.5mL PEI aqueous solution, react under shaking conditions for 3h, carry out magnetic separation to the obtained reaction solution and collect the separated solid product, and then wash the solid product 3 times with deionized water to obtain PEI coated Fe 3 o 4 Magnetic nanoparticles (MNs@...

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Abstract

The invention discloses a magnetic nanoparticle and an immunomagnetic nanoparticle with a cell-like structure as well as a preparation method and application thereof. The magnetic nanoparticle with the cell-like structure uses a superparamagnetic Fe3O4 nanoparticle as a core and is wrapped with a white cell membrane on the surface, so that the magnetic nanoparticle has excellent magnetic responseperformance, furthermore, the white cell membrane wrapping on the surface of the magnetic nanoparticle is homologous with white cells, thereby effectively weakening non-specific adsorption to the white cell; an antibody is modified onto the white cell membrane wrapping on the surface of the magnetic nanoparticle through mediating of lipid molecules-polyethylene glycol-biotin molecules and avidin,so as to obtain the immunomagnetic nanoparticle with the cell-like structure. CTCs cells can be enriched and separated with high efficiency and high purity.

Description

technical field [0001] The invention belongs to the technical field of bionic immunomagnetic nanometer materials, and relates to a magnetic material with a cell-like structure, a preparation method and an application of the magnetic material in the enrichment of circulating tumor cells. Background technique [0002] Circulating tumor cells (CTCs) refer to tumor cells shed from solid tumors into the blood circulation. CTCs spread to other tissues or organs of the body through the circulatory system and play an important role in tumor metastasis and recurrence. The detection of CTCs can provide indicative information for cancer treatment, cancer metastasis and cancer prognosis. Therefore, the detection of circulating tumor cells in peripheral blood has important clinical application value. [0003] However, the concentration of CTCs in blood is extremely low (1mL blood contains only one to dozens of CTCs), which seriously hinders the counting and further research of CTCs. At...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54326G01N33/54346
Inventor 吴尧周小熙易强英张宇佳康珂
Owner SICHUAN UNIV
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