Amido modified magnetic nano-particle and method for preparing immune magnetic nano-separation agent

A magnetic nanoparticle and magnetic nanoparticle technology, which is applied in the field of preparation of immunomagnetic nano-separation reagents, can solve the problems of unsuitability for industrial production and low yield, and achieve the effects of improved synthesis efficiency, good dispersion, and fast magnetic separation

Inactive Publication Date: 2008-09-24
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this invention is still in the laboratory stage. Although the particle size and shape of the product prepared by the inverse microemulsion method are uniform, the yield is extremely low, which is not suitable for industrial production.

Method used

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  • Amido modified magnetic nano-particle and method for preparing immune magnetic nano-separation agent
  • Amido modified magnetic nano-particle and method for preparing immune magnetic nano-separation agent
  • Amido modified magnetic nano-particle and method for preparing immune magnetic nano-separation agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Prepare 1250ml 0.5M NaOH and 125ml 1M FeCl respectively 3 , 125ml 0.5M FeCl 2 and 125ml 0.4M HCl solution, NaOH solution was placed in a three-necked flask, under N 2 Heating the constant temperature water bath to 60°C under protective conditions, the prepared FeCl 3 , FeCl 2 After mixing with HCl solution, it was quickly added to a three-necked flask, stirred at 50 rpm for 15 min, and then cooled to room temperature. Washed 5 times with deionized water and methanol successively, the resulting product Fe 3 o 4 Magnetic nanoparticles are dispersed in methanol. The TEM image of the obtained product is shown in figure 1 Shown in (a); And detect the magnetic property of product, as figure 2 The hysteresis curve is shown.

[0036] 2) The product prepared in the previous step (Fe dispersed in methanol 3 o 4 Magnetic nanoparticles, Fe 3 o 4 Magnetic nanoparticles (20 g) are placed in a flask, a total of 1000 ml of toluene and methanol are added at a volume rati...

Embodiment 2

[0041] 1) Prepare 1250ml 0.5M KOH and 125ml 1M Fe respectively 2 (SO 4 ) 3 , 125ml 0.5M FeSO 4 and 125ml of 0.4M HCl solution, the KOH solution was placed in a three-necked flask, heated to 70°C at a constant temperature under the protection of argon (Ar), and the prepared Fe 2 (SO 4 ) 3 , FeSO 4 After mixing with HCl solution, it was quickly added into a three-necked flask, stirred vigorously at 100 rpm for 20 min, and then cooled to room temperature. Washed several times with deionized water and ethanol successively, the obtained product Fe 3 o 4 Magnetic nanoparticles are dispersed in ethanol.

[0042] 2) Put 20 g of magnetic nanoparticles prepared in the previous step in a flask, add 1050 ml of toluene and ethanol at a volume ratio of 1:15, add 10 ml of sodium silicate, and heat up the flask to 100° C. Stir vigorously at 100 rpm, reflux for 24 hours, and wash with toluene several times to obtain Fe coated with silica gel. 3 o 4 magnetic nanoparticles.

[0043] ...

Embodiment 3

[0047] 1) Prepare 1250ml 0.5M KOH and 125ml 1M Fe respectively 2 (SO 4 ) 3 , 125ml 0.5M FeSO 4 and 125ml 0.4M HCl solution, put the KOH solution in a three-necked flask, under N 2 Heating at constant temperature to 90°C under protective conditions, the prepared Fe 2 (SO 4 ) 3 , FeSO 4 After mixing with HCl solution, it was quickly added into a three-necked flask, stirred vigorously at 80 rpm for 30 min, and then cooled to room temperature. Wash several times with deionized water and methanol successively, and the obtained product Fe 3 o 4 Magnetic nanoparticles were dispersed in methanol.

[0048] 2) Put 20 g of the magnetic nanoparticles prepared in the previous step in a flask, add 1000 ml of toluene and methanol at a volume ratio of 1:30, add 200 ml of tetraethyl orthosilicate, and heat up the flask to 120° C. 2 Protected, stirred vigorously at a speed of 80rpm, refluxed for 30h, and washed several times with toluene to obtain Fe coated with silica gel. 3 o 4 ma...

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Abstract

The invention discloses a preparation method of magnetic nano-particles modified by aminogen. The preparation method comprises the steps that: (1) the alcoholysis of the magnetic nano-particles is carried out in a mixed solvent of toluene and low class monohydric alcohol, and surfaces of the magnetic nano-particles are coated by silica gel; the magnetic nano-particles coated by the silica gel are obtained; (2) the magnetic nano-particles coated by the silica gel react with a silane coupling agent, and the magnetic nano-particles coated by the silica gel with surfaces to modify an amino functional group are made. The invention also discloses a preparation method of an immunity magnetic nano separation agent which comprises the preparation method. The preparation method of the invention has the advantages of stable and moderate reaction system, simple and controllable operation and cheap agent material cost, and the waste reaction solution is easy to treat, and does not pollute environment. The preparation method is adapted to the industrial production. The magnetic nano-particles modified by aminogen has the advantages of small particle size, narrow distribution and good dispersibility, and the immunity magnetic nano separation agent has the advantages of high antibody combining rate, low non-specific binding rate, rapid magnetic separation, etc.

Description

technical field [0001] The invention relates to an amino-modified magnetic nano particle suitable for industrialization and a preparation method of an immunomagnetic nano-separation reagent prepared therefrom. Background technique [0002] In the late 1970s, magnetic separation technology began to be applied in the field of biology, and has achieved remarkable research results in cytology, molecular biology, biochemistry and biomedicine. [0003] Magnetic separation technology uses magnetic particles as a carrier to coat biomolecules such as enzymes, proteins, peptides, antibodies, and antigens. Under the directional control of an external magnetic field, through operations such as affinity adsorption, cleaning, and desorption, complex biological molecules can be separated in one step. The target molecule is separated in the system, which has many advantages such as simple and convenient magnetic separation, high specificity and high sensitivity of affinity adsorption, so it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08C07K1/14C12N15/10
CPCY02P20/582
Inventor 汪勇先梁胜张国欣尹端沚
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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