Method for detecting specific and/or non-specific adsorption of nucleic acids

A non-specific and specific technology used in the detection of nucleic acid specificity and/or non-specific adsorption, which can solve the problems of increased error rate of sequencing results, decreased sequencing throughput and sequencing quality

Pending Publication Date: 2018-06-22
GENEMIND BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These non-specific adsorptions will increase the error rate of sequencing results, resulting in a decrease in sequencing throughput and sequencing quality

Method used

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  • Method for detecting specific and/or non-specific adsorption of nucleic acids
  • Method for detecting specific and/or non-specific adsorption of nucleic acids
  • Method for detecting specific and/or non-specific adsorption of nucleic acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1: Investigating the influence of non-specific adsorption during DNA hybridization

[0092] Chip: glass with epoxy groups on the surface (purchased from Schott);

[0093] Fixed strand 1 (EKB-6P-Cy3): artificially synthesized specific sequence with -NH at the end 3 And the DNA short sequence of the fluorescent group Cy3 dye, the sequence is specifically:

[0094] TTTTTTTTTTTTACTTTGCCTCCTTCTGCATGGTATTTCTTTCTCTTCCGCACCCAG-3' (SEQ ID NO: 1);

[0095] Fixed strand 2 (EKB-7P-Cy3): artificially synthesized specific sequence with -NH at the end 3 And the DNA short sequence of the fluorescent group Cy3 dye, the sequence is specifically:

[0096] TTTTTTTTTTTCCACAAAATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCC TAC-3' (SEQ ID NO: 2);

[0097] Hybrid strand (EKB-6T-Cy5): artificially synthesized complementary pairing with fixed strand 1, non-complementary pairing with fixed strand 2, with a length of 35bp to be tested, a short DNA sequence with a fluorescent group Cy5 at the e...

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Abstract

The invention provides a method for detecting specific and/or non-specific adsorption of nucleic acids. The method comprises the following steps: reacting a to-be-detected nucleic acid with a first probe, wherein the first probe is immobilized on the surface of a first substrate, and the to-be-detected nucleic acid and the first probe are at least partially complementary and have different labels,and the labels can generate signals; detecting signals on the surface of the first substrate to obtain first detection results; reacting the to-be-detected nucleic acid with a second probe, wherein the second probe is immobilized on the surface of a second substrate, the second probe has second labels, and the to-be-detected nucleic acid is not complementary to the second probe; detecting signalson the surface of the second substrate to obtain second detection results; and based on the first detection results and the second detection results, detecting the specific and/or non-specific adsorption of the to-be-detected nucleic acid. The method can detect the specific and/or non-specific adsorption of the nucleic acid and the surfaces of the substrates or the probes on the surfaces of the substrates, and is applicable to production quality control of chips.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a method for detecting nucleic acid specific and / or non-specific adsorption. Background technique [0002] Nucleic acid detection on the chip, for example, using a chip with probes immobilized on the surface to capture nucleic acid and further analyze and detect the captured nucleic acid, including sequence determination, information analysis, etc. Non-specific adsorption will affect the amount of effective data obtained and the accuracy of test results. The qualitative or quantitative detection of non-specific adsorption can determine the quality of the chip, the surface modification of the chip, and be used for prediction of detection results. [0003] For example, in the current nucleic acid sequencing, especially in gene sequencing using chips, there are non-specific conditions when nucleic acid hybridization is performed on the chip, including non-specific ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6837
CPCC12Q1/6837C12Q2523/308C12Q2563/107
Inventor 赵陆洋黄天逊吴增丁颜钦
Owner GENEMIND BIOSCIENCES CO LTD
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