Temperature response type beta-cyclodextrin silica gel stationary phase and preparation method thereof

Abstract

The invention relates to a temperature response type beta-cyclodextrin silica gel stationary phase. The structural formula of the temperature response type beta-cyclodextrin silica gel stationary phase is as shown in the specification. The preparation method comprises the steps of deriving beta-cyclodextrin to obtain a beta-cyclodextrin derivative with a reaction group and a functional group, then performing silylation reaction, modifying a silica gel surface with a silylation reagent with two bonds, performing polymerization reaction on acrylamide and a glycidyl ether monomer to form a meshed temperature-sensitive polymer layer on the silica gel surface through azodiisobutyronitrile serving as an initiator, and finally implanting the beta-cyclodextrin derivative into a polymer chain to obtain the temperature response type beta-cyclodextrin silica gel stationary phase. The preparation process is simple, and the property is stable; the prepared temperature response type beta-cyclodextrin silica gel stationary phase can overcome the shortcomings that silica gel cannot resist water and alkali and undergoes non-specific adsorption; a prepared separation material is novel in structure and flexible in application; effective separation of compounds can be realized by controlling the column temperature; furthermore, the beta-cyclodextrin derivative in the polymer chain can improve the hydrophilicity and the chiral recognition capacity of the stationary phase, and is suitable for separation and chiral separation of polar and hydrophilic compounds and chemical molecules.

Description

technical field [0001] The invention relates to a method for preparing a liquid chromatography stationary phase, in particular, provides a method for preparing a high performance liquid chromatography temperature-responsive silica gel stationary phase. Background technique [0002] Water-soluble, temperature-sensitive polymers are very attractive functional polymer materials in recent years. Since it was discovered in the late 1970s that lightly ionized polyacrylamide has a volume phase transition phenomenon in a specific medium, people have studied a series of factors that cause its volume phase transition, such as temperature, pressure, solvent composition, and pH value. and ionic strength have been studied in depth. In recent years, this kind of temperature-sensitive polymer gel has shown broad application prospects in the fields of controlled release of drugs, separation of substances, and immobilized enzymes. There are continuous reports on the use of heat-sensitive...

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Problems solved by technology

[0004] Nevertheless, the types of temperature-sensitive chromatographic packing are still very limited, and its retention mechanism needs to be further studied. Therefore, it is of great significance to the preparation and application of temperature-sensitive chromatographic packing.

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