High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for determination of indaziflam and metabolites of indaziflam

Abstract

The invention discloses a high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for the determination of indaziflam and metabolites of the indaziflam. The method comprises the steps as follows: establishing a standard working curve of a soil matrix of a known concentration gradient of indaziflam and four metabolites of the indaziflam by the HPLC-MS/MS; determining theresidual amount of the indaziflam and the four metabolites of the indaziflam in a soil sample extraction and purification liquid by the HPLC-MS/MS, recording peak areas of extracted ion chromatograms, quantifying the matrix with an external standard method, obtaining measured values of the indaziflam and the four metabolites of the indaziflam in the soil sample extraction and purification liquid; and then substituting the measured values into a quantitative calculation formula to finally obtain the residual amount of the indaziflam to be detected and the four metabolites of the indaziflam insoil samples. The method provided by the invention has the advantages that the sample pretreatment operation is simple and convenient, the dosage of an organic solvent is small, the target pesticideextraction rate is high, the purification effect is good, and the instrument analysis time is short. In addition, the instrument detection limit and the method detection limit are very low.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a high-performance liquid chromatography tandem mass spectrometry analysis method for indazinflufen and its metabolites in soil. The method is simple, fast, and has high sensitivity and accuracy. Background technique [0002] Triazine herbicides (triazine herbicides) rank second among herbicides and have played a huge role in the world's agricultural production. However, due to their large amount of use and long residual time, weeds are prone to develop resistance and can be harmful to soil. , surface water and other drinking water sources cause pollution, and it is urgent to develop new herbicides to replace traditional triazine herbicides. Therefore, a new type of high-efficiency alkyl triazine herbicide indazinflufen, which has the characteristics of less dosage, wide herbicidal spectrum, long residual effect period, and good weed control effect, came into being...

AI Technical Summary

Problems solved by technology

[0002] Triazine herbicides (triazine herbicides) rank second among herbicides and have played a huge role in the world's agricultural production. However, due to their large amount of use and long residual time, weeds are prone to develop resistance and can be harmful to soil. , surface water and other drinking water sources cause pollution, and it is urgent to develop new herbicides to replace traditional triazine herbicides

At present, there are no relevant reports on the analysis methods of indazine, flurafen and the four metabolites at home and abroad

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