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Low-molecular-weight substance mass spectrometry detection method based on palladium-gold core-shell micro-nano material

A technology of micro-nano materials and low molecular weight, which is applied in the field of mass spectrometry detection of low molecular weight substances, can solve the problems of cumbersome pretreatment steps, difficulty in achieving low cost of serum, and long time consumption, and achieve low cost, high accuracy, and low preparation cost. Effect

Active Publication Date: 2020-07-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the cumbersome and time-consuming pretreatment steps of gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, it is difficult to achieve low-cost, large-sample analysis and detection of serum and apply it to clinical practice
Compared with the above two mass spectrometry methods, matrix-assisted laser desorption time-of-flight mass spectrometer has the characteristics of simple sample preparation and high analysis efficiency, but it has extremely high requirements for the matrix

Method used

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  • Low-molecular-weight substance mass spectrometry detection method based on palladium-gold core-shell micro-nano material
  • Low-molecular-weight substance mass spectrometry detection method based on palladium-gold core-shell micro-nano material
  • Low-molecular-weight substance mass spectrometry detection method based on palladium-gold core-shell micro-nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the detection of glucose standard substance

[0055] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometer, using reflection mode, positive ion detection; prepared micro-nano particles; prepared glucose standard solution;

[0056] (2) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0057] (3) Detect under the mass spectrometer, and analyze the mass spectrum image, and the test results are as follows: Figure 4 shown.

Embodiment 2

[0058] Embodiment 2: the detection of lysine standard substance

[0059] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometer, using reflection mode, positive ion detection; prepared micro-nano particles; prepared lysine standard solution;

[0060] (2) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0061] (3) Detect under the mass spectrometer, and analyze the mass spectrum image, and the test results are as follows: Figure 5 shown.

Embodiment 3

[0062] Example 3: Detection of Small Molecules in Serum Samples

[0063] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometer, using reflection mode, positive ion detection; prepared palladium-gold alloy core-shell micro-nano particles;

[0064] (2) Take 0.1 microliter of serum and dilute it 10 times to prepare a serum sample;

[0065] (3) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0066] (4) Detect under the mass spectrometer, and analyze the mass spectrum image, and the test results are as follows: figure 1 shown.

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Abstract

The invention discloses a low-molecular-weight substance mass spectrometry detection method based on a palladium-gold core-shell micro-nano material, and particularly relates to application of the low-molecular-weight substance mass spectrometry detection method in serum low-molecular-weight substance detection. The palladium-gold alloy core-shell micro-nano particles are used as a matrix material, a serum sample does not need to be subjected to any pretreatment steps such as enrichment or separation, and low-molecular-weight substances in serum can be efficiently and quickly detected and analyzed by taking a very small amount of sample. The detection method is high in accuracy, low in cost and high in detection flux, meets the requirements of clinical serum detection, and has clinical application potential.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry detection of low molecular weight substances, in particular to a detection method and application of serum low molecular weight substances or metabolite mass spectrometry based on palladium-gold core-shell micro-nano materials. Background technique [0002] Diagnosis is the key to screening cancer and can guide clinical treatment. Notably, blood test-based diagnosis offers desirable sensitivity and specificity, is low cost, and is noninvasive compared to imaging and biopsy methods. Metabolic profiling is an emerging precision medicine tool that can detect pathway end products. However, due to sample complexity and low metabolite abundance in patient specimens, metabolic profiling is still insufficient for diagnostic applications. Therefore, there is an urgent need to construct new metabolic analysis tools for the clinical application of cancer screening and treatment. [0003] Compare...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/628
Inventor 钱昆曹敬黄琳苏海洋张梦吉
Owner SHANGHAI JIAO TONG UNIV
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