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Wide-range electron beam excited fluorescence imaging and spectral measurement device and method thereof

A spectrum measurement and fluorescence spectrum technology, which is applied in the field of large-scale electron beam excited fluorescence imaging and spectrum measurement devices, can solve the problems of inability to meet the requirements of fluorescence imaging analysis, uneven collection efficiency of fluorescence intensity, etc., and achieve high-resolution imaging, high The effect of fluorescence collection efficiency

Active Publication Date: 2018-07-10
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the collection efficiency of fluorescence intensity at each position is not uniform under a large-scale field of view, that is, under the large-scale electron beam scanning imaging in a scanning electron microscope, it cannot meet the required fluorescence imaging analysis requirements

Method used

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  • Wide-range electron beam excited fluorescence imaging and spectral measurement device and method thereof
  • Wide-range electron beam excited fluorescence imaging and spectral measurement device and method thereof
  • Wide-range electron beam excited fluorescence imaging and spectral measurement device and method thereof

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Embodiment Construction

[0031] The present invention will be further described through the embodiments below in conjunction with the accompanying drawings.

[0032] like figure 1 As shown, the large-scale electron beam excited fluorescence imaging and spectrum measurement device of this embodiment includes: scanning electron microscope system, scanning signal generator, large-scale fluorescence collection coupling transmission system, fluorescence intensity detector, fluorescence spectrum detector, scanning synchronization Signal collector, collaborative control and data processing output system; among them, the collaborative control and data processing output system serves as a synchronous control and data acquisition center, and is connected with scanning electron microscope system, scanning signal generator, large-scale fluorescence collection coupling transmission system, fluorescence intensity The detector, the fluorescence spectrum detector and the scanning synchronous signal collector are conn...

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Abstract

The invention discloses a wide-range electron beam excited fluorescence imaging and spectral measurement device and a method thereof. The wide-range electron beam excited fluorescence imaging and spectral measurement device comprises a electron microscope scanning system, a scanning signal generator, a wide-range fluorescence collecting, coupling and transmitting system, a fluorescence intensity detector, a fluorescence spectral detector, a synchronous scanning signal collector and a cooperative control and data processing output system. Since a wide-range fluorescence coupler is introduced, fluorescence signals excited within an imaging view field by the microscope scanning system can be converged and coupled to a fluorescence transmission light path with high collection efficiency, the problems that, to images acquired by wide-range fluorescence scanning imaging, it is difficult to calculate and compare fluorescence excitation intensity or fluorescence yield and fluorescence spectruminformation of different positions according to uniform standards are solved, and wide-range rapid detection and analysis on the basis of electron beam excited fluorescence signals can be completed.

Description

technical field [0001] The invention relates to electron beam excited fluorescence signal detection and processing technology, in particular to a large-scale electron beam excited fluorescence imaging and spectrum measuring device and method thereof. Background technique [0002] The fluorescent signal excited by the electron beam refers to the electromagnetic waves emitted by the electron beam in the ultraviolet, infrared or visible light band except for secondary electrons, backscattered electrons, Auger electrons and X-rays when the electron beam bombards the surface of the material; The basic principle is that the electrons inside the material are excited by the incident electrons to a high-energy state, and after a certain relaxation time, they transition back to a low-energy state and release energy, and part of the energy is emitted in the form of electromagnetic radiation. The physical process of a material producing fluorescence under electron beam excitation is det...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/6402G01N2021/0131G01N2021/0162G01N2021/6423
Inventor 朱瑞徐军刘亚琪
Owner PEKING UNIV
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