46results about How to "Improve fluorescence collection efficiency" patented technology

A micro flow control capillary electrophoresis liquid core waveguide fluorescence detector includes a liquid core waveguide capillary, a laser light source, a photodetector, a high-voltage power supply, an electrode, a liquid storage container, a sample tube, a pinhole diaphragm, a filter and a shadow shield; the device makes use of a liquid core waveguide phenomenon and uses the same liquid corewaveguide capillary as a capillary electrophoresis separation channel and for fluorescence signal transduction. In the invention, the light given out by the laser light source can be not focused by alens but directly radiates on the liquid core waveguide capillary through the pinhole diaphragm; the fluorescence led out by the outlet port of the liquid core waveguide capillary can not be collectedbythe lens but directly enters into the photodetector through the filter. The liquid core waveguide capillary is taken as a micro flow control capillary electrophoresis separation channel and an optica l conduction channel, so as to furthest reduce optical elements, simplify an optical system, make the structure more compact, so as to provide a feasible technical route for developing miniature microflow control analytical instruments.

The invention relates to the technical field of optical microscopic imaging, and in particular relates to a three-dimensional head-mounted microscope which comprises a lens assembly, an objective lensand a scanner assembly. A photoelectric detector is arranged on the objective lens and comprises a protection element, an optical filter, a photoelectric sensitive unit and a driving circuit. According to the scheme, related elements for fluorescence collection are simplified, so that the sizes of the related elements for fluorescence collection are smaller, and meanwhile, related experimental operation and detection can be carried out.

The invention is a biochip detecting system, composed of semiconductor cooling CCD camera, high-efficiency object lens, X-Y 2-D table, bar code detect-read head, filament lamp, camera bellows, and computer. Use the bar code detect-read head to scan the biochip with chemical luminescence label, make the computer resister by identifying sample, then place the sample on the X-Y planar table, adjust the planar table to make the sample at the center of the object lens, close the door of the camera obscura to make the sample in the camera obscura, image the chemical luminescence signal on the CCD camera, and set the CCD parameters by software in the computer, like time of exposure, resolution, etc, to start collecting the chemical luminescence image.

The invention relates to a liquid core waveguide detector, in particular to a liquid core waveguide fluorescence detector, which comprises a liquid core optical fiber, an exciting light source, and a fluorescence detection component. The side wall of the liquid core optical fiber is provided with an incident window; exciting liquid emitted by the exciting light source is emitted into the liquid core optical fiber at a proper angle through the incident window on the side all of the liquid core optical fiber, and is spread in a total reflection way along the axis direction of the liquid core optical fiber; a test sample in a liquid medium in the liquid core optical fiber is excited and emits fluorescence; and the fluorescence detection component detects the fluorescence transmitted through the side wall of the liquid core optical fiber. The liquid core waveguide detector has the advantages that: the exciting light and the fluorescence are separated by a liquid core waveguide principle, and the background noise of the detector can be reduced effectively; the exciting light is spread in a capillary tube along the axis, the exciting optical path and utilization rate are greatly increased, and the sensitivity of the detector is improved; meanwhile, the device is simple in structure, convenient to use, low in cost and wide in application range.

The disclosure provides a vacuum device for a miniaturized atomic interferometer, which includes upper and lower windows, a square cavity, a connection part, an atom source part, a three-dimensional magneto-optical trap part, an interference part and a detection part. The upper and lower windows and the fasteners are sealed with thick glass coated with anti-reflection film on both sides, which can greatly reduce the system error caused by the wavefront distortion effect. Through the reasonable design of the optical windows other than the upper and lower windows and direct embedded welding into the vacuum cavity, and the adoption of a reasonable atomic cooling interference scheme, the volume and weight of the vacuum device are greatly reduced, and the structure becomes simple. reliable. At the same time, the vacuum structure is also suitable for applications such as cold atom gyroscopes and gravity gradiometers after appropriate modification.

The invention proposes a fluorescence detection device, which includes a detection cell, a transmitting optical fiber, a focusing lens, a receiving optical fiber and a receiver; the detection cell is cylindrical, the side wall of the detection cell is a transparent side wall, and the upper end of the detection cell is an opening The focusing lens is installed above the opening of the detection pool; one end of the emitting fiber corresponds to the upper position of the focusing lens, and the other end of the emitting fiber is used to be installed on the excitation light source; the receiving optical fiber includes a winding part and a transmission part, the The winding part has a core, the transmission part has a core and a cladding; the winding part is wound outside the side wall of the detection cell, and the end of the transmission part is coupled with the receiver. The fluorescence detection device of the present invention can effectively improve fluorescence collection efficiency, receive fluorescence in all directions, and improve detection accuracy; and has the advantages of small size and easy implementation.

The invention relates to a fluorescence collecting object lens of a biological chip scanner, which is based on a CCD or CMOS imaging method. The fluorescence collecting object lens comprises eight lenses, and fluorescence signals successively penetrate through a plano-convex positive lens J1, a double-concave negative lens J2, a concave-convex positive lens J3, a plano-concave positive lens J4, a plano-convex positive lens J5, a concave-convex negative lens J6, a plano-plane lens J7 and a convex-concave positive lens J8 from an object side to a CCD imaging surface, wherein the lenses are arranged on a optical axis, the double-convex positive lens J5 and the concave-convex negative lens J6 form a double gummed lens, an aperture diaphragm is arranged on the surface of the plano-convex positive lens J4 close to the object side, and the plano-plane lens J7 is an optical filter of emission fluorescence. The fluorescence collecting object lens of the biological chip scanner provided by the invention is simple in structure, low in cost, and large in view field, can rapidly increase scanning speed, substantially improves the fluorescence collection efficiency and detection flexibility, and can be suitable for all visible optical bands.