46results about How to "Improve fluorescence collection efficiency" patented technology

Exemplary embodiments include systems, methods, and compositions for the intra-operative detection of target tissue. At least one embodiment includes a fluorescence detection instrument that may be used for intra-operative detection of a fluorescent targeting agent, its binding site, and its interaction within cancer tissues. An exemplary embodiment is highly sensitive to the local deposition of fluorescence agents even at a low concentration. In at least one embodiment, the system includes a handheld navigation instrument that is usable to excite, detect, and report the fluorescent deposition of the targeting agent in real-time. In alternative embodiments, the system includes a wearable unit to excite, detect, and visually report the fluorescent deposition of the targeting agent to the user. The wearable unit includes eyewear that allow the user to perform image-guided surgery based on the near real-time fluorescence detection of the fluorescent targeting agent.

A magnetometer includes a diamond sensor, an excitation light source, a diamond sensor case, and a photodiode. The excitation light source irradiates the diamond sensor case with excitation light. In the diamond sensor case, a reflection film which reflects excitation light is formed on either a front surface or an inner surface, and the diamond sensor is stored. The photodiode detects intensity of fluorescence generated from the diamond sensor. The diamond sensor case includes a fluorescence output window and an excitation-light reception window. Fluorescence generated by the diamond sensor is output through the fluorescence output window. Excitation light emitted by the excitation light source is received through the excitation-light reception window. The photodiode is provided on a side of a second surface opposite to a first surface which is a magnetism measurement surface of the diamond sensor.

The invention relates to a real-time online optical fiber oxygen sensor. A high power light-emitting diode or pulse lamp is adopted as the excitation light source. The excitation light is transmitted by an exciting optical fiber to irradiate an oxygen fluorescence sensing membrane, and the generated fluorescence is collected by a launching optical fiber after passing through an optical filter and is transmitted to an optoelectronic conversion device to be detected. In a probe of the optical fiber oxygen sensor, the exciting optical fiber and the launching optical fiber forms an angle of 50-70 degrees, thus effectively reducing interference of media. The maximal fluorescence receiving efficiency can be obtained near the angle of 60 degrees. The oxygen fluorescence sensing membrane is placed in the sensor and can continuously detect the oxygen content in the gas and the dissolved oxygen in the water on line in real time. The sensor has the advantages of long service life, wide measurement range, high sensitivity, good stability and repeatability, easy miniaturization and the like.

The invention provides a flow-through fluorescence detection cell. The flow-through fluorescence detection cell comprises an upper clamping member, a lower clamping member and a quartz tube. The upper clamping member has a cylindrical structure, the lower end surface of the upper clamping member is provided with a through rectangular upper groove, and the upper end surface of the upper clamping member is downward vertically provided with a light through hole; the top of the lower clamping member is provided with a through rectangular lower groove; and the quartz tube is clamped between the upper groove and the lower groove as a flow-through cell, and the through directions of the upper groove and the lower groove parallel to the axial direction of the quartz tube. Light from a light source goes through the through hole and irradiates to the quartz tube, and strongly reflected excitation light generated by an air-quartz tube external surface optical interface is blocked by two inner side edges of the upper groove, and cannot be propagated to a fluorescence collection light path, so the high background noise caused by reflected excitation light is effectively weakened, and the detection signal-to-noise ratio is improved. The detection cell has the advantages of simple structure and easy manufacturing, and is suitable for capillary electrophoresis, liquid chromatography and flow analysis fluorescence detectors.

The invention discloses a cold atom number detecting device. The cold atom number detecting device comprises a first detector, a second planoconvex lens, a first planoconvex lens, a third planoconvex lens, a fourth planoconvex lens, a second detector, a reflector, an adapting light barrel, an optical slit, a beam-expanding light barrel, a beam expanding lens, a quarter wave plate, a first polarization beam splitter, a half wave plate, a second polarization beam splitter and a detecting chamber; the cold atom number detecting device is simple in structure and convenient to operate and can realize three-dimensional adjustment of the position of the detector, and therefore, the cold atom number detecting device facilitates detection of cold atomic fluorescence signals; the cold atom number detecting device is designed modularly, can be conveniently used for the cold atom number detection of different detection systems, the cold atom number detecting device adopts heavy-caliber and short-focal-length lens combination for bi-directional detection, the fluorescence collecting efficiency is high and the signal to noise ratio is increased.

The invention belongs to the bioanalysis technical field, and relates to a rotational scanning capillary array analyzer. The analyzer comprises a laser light source, a scanning reflector, a scanning objective, a detection window fixing device, a capillary sampling end, a capillary exhaust liquid end, a light beam steering system, a fluorescence spectroscope, a fluorescence color filter, an optical detector and a scanning central shaft, wherein, a plurality of capillaries are arranged around the scanning central shaft to form a cylindrical array, and the capillary array is fixedly arranged in the detection window fixing device; laser beam of the light source sequentially passes through the fluorescence spectroscope, the scanning reflector, the light beam steering system and the scanning objective, and the scanning objective converges the laser beam on the detection window; a biological sample marked with fluorescence in the capillary is activated by the laser, irradiated fluorescence passes through the scanning objective, the light beam steering system, the fluorescence spectroscope and the fluorescence color filter, and is detected by the optical detector. The rotational scanning capillary array analyzer has the advantages of high flux, high sensitivity, large fluorescence collection angle and good performance.

According to the diamond AFM probe system and the manufacturing method thereof. The diamond AFM probe system is a novel diamond AFM probe system and has high fluorescence collection efficiency, so thedetection sensitivity of the diamond AFM probe system is improved, and the manufacturing method is simple.

The invention discloses a rapid detecting device and method for a latent fingerprint. The rapid detecting device comprises a light source and transmitting module, a dichroic mirror, a flying scanningmodule, a sample platform, a fluorescence detection module and an overall machine control module; and the rapid detecting method comprises two modes of rough detecting and fine detecting, the rough detecting mode is used for rapidly positioning a suspicious fingerprint area on a detecting material, and the fine detecting mode is used for performing high-resolution detection on the suspicious fingerprint area to obtain precise fingerprint information. In the detecting process, a light source forms a one-dimension focal line on the detecting material, and a linear array detector is used for receiving fluorescence; and the light source and transmitting module, the dichroic mirror and the fluorescence detection module maintain to be stationary, and an optical module moving bearing platform drives a mirror and a focusing collimating mirror module to perform one-dimension flying scanning linear motion. According to the detecting device and method for the latent fingerprint, the detecting speed is high, an obtained fingerprint fluorescent image is not distorted, and the precision of the detecting result is improved.

The invention discloses an electron beam excitation fluorescence large-range direct detection imaging apparatus and a method thereof. The imaging apparatus comprises a scanning electron microscope system, a scanning signal generator, a fluorescence collection coupling system, a semiconductor photodetector, a scanning synchronization signal collector and a synergic control and data processing output system. According to the present invention, by using the modular architecture, the configuration adjustment and the subsequent upgrade of each module are flexible and convenient; and by introducingthe large-area semiconductor photoelectric detection chip of the semiconductor photodetector, the fluorescence excited by the scanning electron microscope system in the large imaging visual field range can be concentrated and coupled to the semiconductor photodetector at the same high collection efficiency, such that the problem that the fluorescence excitation intensities or the fluorescence excitation yields at different positions of the image obtained through the large-range fluorescence scanning imaging are difficultly calculated and compared by using the standard is solved so as to complete the large-range rapid detection and analysis based on the electron beam excitation fluorescence signals.

The invention provides a fluorescence detection device which comprises a detection pool, an emitting fiber, a focusing lens, a receiving fiber and a receiver, wherein the detection pool is cylindrical, a side wall of the detection pool is a transparent side wall, and an opening is formed at the upper end of the detection pool; the focusing lens is mounted above the opening of the detection pool; one end of the emitting fiber corresponds to a position above the focusing lens, and the other end of the emitting fiber is mounted on an excitation light source; the receiving fiber comprises a winding part and a transferring part, the winding part is provided with a fiber core, and the transferring part is provided with a fiber core and a coating layer; the winding part winds outside the side wall of the detection pool, and the tail end of the transferring part is coupled with the receiver. The fluorescence detection device provided by the invention can effectively improve the fluorescence collection efficiency, receive fluorescence from all dimensions and improve the detection precision, and has the advantages of small volume and facilitation of implementation.

The invention provides a differential acceleration sensing device based on NV color center solid spinning. The device comprises a symmetrical structure, NV color center sensing modules and a detection module, wherein the symmetrical structure comprises a magnetic source, an elastic beam and a symmetrical substrate, and the two NV color center sensing modules are fixed to the surfaces, perpendicular to the z axis, of protruding parts on the two sides of the symmetrical substrate respectively. According to the working principle, under the action of external acceleration, the elastic beam in the symmetrical structures deform to convert acceleration into three-axis displacement variation of the magnetic source along the x axis, the y axis and the z axis, then a magnetic field at the NV color center sensing module is influenced, the NV color center electron spinning energy level is changed, polarization and control of spinning of the NV color center are achieved through laser and microwaves of the detection module, magnetic field information is solved by reading double-path fluorescence signals released by the NV color center, and differential measurement of acceleration is achieved. According to the invention, the advantage of high sensitivity of NV color center magnetic measurement is utilized, and through reasonable design of a symmetrical structure, differential detection of acceleration is realized, and the acceleration measurement precision is improved.

The invention relates to a fluorescence collecting object lens of a biological chip scanner, which is based on a CCD or CMOS imaging method. The fluorescence collecting object lens comprises eight lenses, and fluorescence signals successively penetrate through a plano-convex positive lens J1, a double-concave negative lens J2, a concave-convex positive lens J3, a plano-concave positive lens J4, a plano-convex positive lens J5, a concave-convex negative lens J6, a plano-plane lens J7 and a convex-concave positive lens J8 from an object side to a CCD imaging surface, wherein the lenses are arranged on a optical axis, the double-convex positive lens J5 and the concave-convex negative lens J6 form a double gummed lens, an aperture diaphragm is arranged on the surface of the plano-convex positive lens J4 close to the object side, and the plano-plane lens J7 is an optical filter of emission fluorescence. The fluorescence collecting object lens of the biological chip scanner provided by the invention is simple in structure, low in cost, and large in view field, can rapidly increase scanning speed, substantially improves the fluorescence collection efficiency and detection flexibility, and can be suitable for all visible optical bands.