Fluorescence enhanced chip based on all-medium artificial microstructure super-surface

Abstract

The invention discloses a fluorescence enhanced chip based on an all-medium artificial microstructure super-surface. An upper layer of the chip is composed of periodically-arranged nano-medium columnclusters, and a lower layer is a medium substrate; the refractive index of the upper layer is greater than that of the lower layer; the collective magnetic dipole resonance wavelength and the collective electric dipole resonance wavelength of the chip satisfy that one is equal to the excitation wavelength of a fluorescent marker, and the other is equal to the radiation wavelength of the fluorescent marker. In the periodically-arranged nano-medium column clusters, each of periodic units is a square or a regular hexagon of the same size; four nano-medium columns of the same size are contained ineach of the periodic units. The fluorescence enhanced chip disclosed by the invention has the benefits that a fluorescence signal is simultaneously enhanced from three aspects of enhancing an excitation light local field, improving the radiation quantum efficiency and improving the fluorescence collection efficiency, so that the detection sensitivity of the fluorescent marker is greatly improved.The whole chip is arranged on a low-refractive index substrate and has the advantages that the material loss is low, the preparation is simple, and the structure size can be changed to work in different wavebands.

Description

technical field [0001] The invention belongs to the field of surface-enhanced fluorescence signals, and in particular relates to a fluorescence-enhanced chip based on an all-dielectric artificial microstructure metasurface, which is mainly used for detecting substances marked by fluorescent molecular groups, identifying substances, and analyzing the properties of substances. Under the excitation of visible light or near-infrared light source, the fluorescence enhancement chip expresses in the form of visible and near-infrared light through the process of up-conversion or down-conversion fluorescence, and can realize rapid and sensitive qualitative and quantitative detection of the sample to be tested. Background technique [0002] In molecular biology, biochemistry, protein chip technology and other life science fields, people often use fluorescent markers to achieve quantitative and qualitative detection of specific substances (such as nucleic acids, antigens, enzymes, etc.)...

AI Technical Summary

Problems solved by technology

However, metals have a large material loss in the visible and near-infrared bands, especially when the fluorescent marker is too close to the metal structure (less than 15 nanometers), its fluorescence efficiency will be greatly reduced, this phenomenon is also called fluorescence quenching

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