Method and device for enhancing fluorescence intensity of gold nanosphere
A technology of gold nanospheres and fluorescence intensity, which is applied in the field of nanomaterials and optics, can solve the problems of difficult industrial production, limited enhancement effect, and high cost, and achieve the effects of low cost, enhanced effect, and improved diagnostic accuracy
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[0026] A method for enhancing the fluorescence intensity of gold nanospheres in this embodiment comprises the following steps:
[0027] 1) Preparation of agglomerated gold nanosphere sample 4:
[0028] A piece of cover glass was taken, washed with acetone, potassium hydroxide and deionized water respectively, and the gold nanosphere solution was spin-coated on the cover glass by spin coating method to prepare agglomerated gold nanosphere sample.
[0029] The thickness of the cover glass is 0.17 mm.
[0030] The gold nanospheres are prepared by a seed-mediated growth method, the obtained gold nanospheres have a diameter of about 160 nanometers, and the optical density of the gold nanosphere solution used for spin coating is 0.005.
[0031] The specific parameters of the spin-coating method are: 1. first spin-coating with a speed of 500 rpm for 10 seconds; 2. then spin-coating with a speed of 2000 rpm for 20 seconds; Second.
[0032] figure 2 The transmission electron micro...
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