A two-photon fluorescence enhancement method and its application

A two-photon fluorescence and fluorescent material technology, applied in the field of optoelectronics, can solve the problems of unfavorable optical signal transmission and utilization, unfavorable low-cost micro-nano composite structure, complex process, etc., and achieve the effect of low cost, wide application range and simple process

Active Publication Date: 2021-08-03
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods often require the use of micro-nano processing methods such as electron beam etching and focused ion beam etching to prepare some complex micro-nano composite structures. On the one hand, these preparation processes are complicated and costly, which is not conducive to the preparation of low-cost , large-area micro-nano composite structure; on the other hand, the noble metal composite structure itself has a large optical loss in the visible light band, which is not conducive to the transmission and utilization of optical signals
All in all, the current methods and means are complex and costly, and still have great limitations for practical applications

Method used

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  • A two-photon fluorescence enhancement method and its application
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  • A two-photon fluorescence enhancement method and its application

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Effect test

Embodiment 1

[0048] A method 100 for enhancing two-photon fluorescence, such as figure 1 shown, including:

[0049] Step 110, stacking and combining the fluorescent material and the dielectric microspheres to obtain a composite dielectric structure.

[0050] Step 120 , irradiating the laser light emitted by the laser source onto the dielectric microspheres in the composite dielectric structure through a microscope objective lens for focusing to obtain a focused light source.

[0051] Step 130, the focused light source excites the fluorescent material to realize enhancement of two-photon fluorescence of the fluorescent material.

[0052] Due to the photon jet effect of the dielectric microspheres, the light beam can be focused on the sub-wavelength scale, so that the light intensity in the focused area is greatly increased. For two-photon fluorescence, the fluorescence intensity is proportional to the square of the excitation light intensity. If a dielectric microsphere is used to focus the...

Embodiment 2

[0055] On the basis of Embodiment 1, in the composite dielectric structure, the dielectric microspheres are a single dielectric microsphere or an array of dielectric microspheres.

[0056] A single microsphere or a microsphere array can greatly enhance the two-photon fluorescence intensity of the fluorescent material, so it has a wide range of applications and can be used according to actual conditions, which is convenient and quick. Among them, a single dielectric microsphere or a dielectric microsphere array can be prepared by the following method: take a small amount of suspension of dielectric microspheres and drop them on a clean quartz substrate and place them in an oven with a temperature of 70°C. Under the action of liquid tension, the dielectric microspheres form a single-layer dense microsphere array or dispersed single dielectric microspheres by self-assembly, and keep them in the oven for about 10 minutes to dry them completely. A single dielectric microsphere or a...

Embodiment 3

[0058] On the basis of embodiment one or embodiment two, when the dielectric microsphere is a single dielectric microsphere, such as figure 2 As shown, before step 110, method 100 also includes:

[0059] Step 140: Determine the microsphere size and microsphere material according to the preset two-photon fluorescence enhancement factor and fluorescent material, and obtain a single dielectric microsphere corresponding to the microsphere size and microsphere material;

[0060] or,

[0061] When the dielectric microsphere is a dielectric microsphere array, such as image 3 As shown, before the step 110, the method 100 also includes:

[0062] Step 150: Determine the microsphere size and microsphere material according to the preset two-photon fluorescence enhancement factor and fluorescent material, and obtain a dielectric microsphere array composed of a plurality of single dielectric microspheres corresponding to the microsphere size and microsphere material .

[0063] Accordi...

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Abstract

The invention relates to a method for enhancing two-photon fluorescence and its application. The method includes: stacking and combining fluorescent materials and dielectric microspheres to obtain a composite dielectric structure; Focusing is carried out on the dielectric microspheres in the structure to obtain a focused light source; the focused light source excites the fluorescent material to realize the enhancement of two-photon fluorescence of the fluorescent material. In the present invention, a composite dielectric structure is prepared by combining the fluorescent material with the dielectric microspheres, and through the focusing of the light source by the dielectric microspheres in the structure, the fluorescence intensity of the fluorescent material can be enhanced by about 100 times under the same laser power Compared with the existing technology for enhancing two-photon fluorescence, the technical solution of the present invention has the advantages of low cost, simple process, applicable to large-area solid-state fluorescent materials, etc., and effectively solves the problem of low two-photon fluorescence efficiency of current fluorescent materials.

Description

technical field [0001] The invention relates to the field of optoelectronic technology, in particular to a method for enhancing two-photon fluorescence and its application. Background technique [0002] At present, the two-photon fluorescence efficiency of fluorescent materials, especially nanomaterials, is often relatively low, which greatly limits its practical application. In the past, people usually use methods such as surface plasmon resonance to enhance the interaction process between light and matter, thereby increasing the efficiency of two-photon fluorescence. However, these methods often require the use of micro-nano processing methods such as electron beam etching and focused ion beam etching to prepare some complex micro-nano composite structures. On the one hand, these preparation processes are complicated and costly, which is not conducive to the preparation of low-cost , Large-area micro-nano composite structure; on the other hand, the noble metal composite s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6458
Inventor 刘为为王凯龙华王兵陆培祥
Owner HUAZHONG UNIV OF SCI & TECH
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