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Detector of fluorescence induced by light-emitting diode in high brightness

A light-emitting diode and induced fluorescence technology, which is applied in fluorescence/phosphorescence, color/spectral characteristic measurement, instruments, etc., can solve the problems of limited fluorescence collection efficiency, complex structure, and inability to use fluorescence collection lenses, etc.

Inactive Publication Date: 2006-02-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although this structure can reduce excitation light interference to a certain extent, its disadvantages are complex structure, large volume, and high cost.
These shortcomings determine that the orthogonal optical structure is not conducive to the miniaturization or miniaturization of the system
In addition, limited by steric hindrance, fluorescence collection lenses with large numerical apertures cannot be used in the orthogonal structure, so the fluorescence collection efficiency is limited

Method used

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  • Detector of fluorescence induced by light-emitting diode in high brightness
  • Detector of fluorescence induced by light-emitting diode in high brightness
  • Detector of fluorescence induced by light-emitting diode in high brightness

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Embodiment

[0022] The light-emitting diode-induced fluorescence detector of the present invention is used to detect phenylalanine labeled with fluorescein isothiocyanate in capillary electrophoresis.

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Abstract

An induced fluorescence detector of high brightness LED is composed of exciting light source, lens set, and beam splitting mirror, filter, photoelectric converting component and detection cell. it is featured as setting fluorescent collection light path and exciting light path at the same side of detection cell in coaxial optical structure and setting 45 degree angle of beam splitting mirror to axis of exciting light path and fluorescent collection light path .

Description

technical field [0001] The invention relates to the technical field of trace detection, and is a light-emitting diode-induced fluorescence detector, which is mainly applicable to the detection of trace samples in the fields of biology, environment, food monitoring and the like. Background technique [0002] As a photoluminescent detection technology, fluorescence detection has high sensitivity and high selectivity, and is widely used in the detection of micro and trace samples. As a key component of a fluorescence detection system, the excitation light source is often one of the most expensive components of the system. Gas discharge lamps and lasers are the most widely used excitation sources for fluorescence detection. However, this light source has the disadvantages of large size, high energy consumption and short life. As an economical miniature light source, high-brightness light-emitting diodes (LEDs) have gradually attracted people's attention in recent years. High-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/25G01N30/02
Inventor 关亚风杨丙成谭峰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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