A flow-through fluorescence detection cell

Abstract

The invention provides a flow-through fluorescent detection pool, which is composed of an upper clamp, a lower clamp and a quartz tube. The upper clamping part has a cylindrical structure, the lower end surface is provided with a through rectangular upper groove, and the upper end face is vertically provided with a light hole; the top of the lower clamping part is provided with a penetrating rectangular lower groove; the quartz tube is clamped in the flow cell Between the upper groove and the lower groove, the penetrating directions of the upper groove and the lower groove are parallel to the axial direction of the quartz tube. When the light source is irradiated onto the quartz tube through the hole, the strong reflected excitation light generated by the optical interface on the outer surface of the air-quartz tube is blocked by the two inner edges of the upper groove, and cannot be transmitted into the fluorescence collection optical path, thus effectively weakening the reflected excitation light The resulting high background noise improves the detection signal-to-noise ratio. The detection cell has a simple structure and is easy to manufacture, and is suitable for capillary electrophoresis, liquid chromatography and flow analysis fluorescence detectors.

Description

technical field

[0001] The patent of the present invention relates to the technical field of fluorescence detectors, more specifically, to a flow-through fluorescence detection cell. Background technique

[0002] Liquid chromatography and capillary electrophoresis, as high-efficiency separation techniques, are widely used in the separation and analysis of mixed substances. Fluorescence detector (FLD) is a highly sensitive and highly selective detector, combined with liquid chromatography, capillary electrophoresis and fluorescence detector, widely used to detect complex mixed trace biological and environmental substances. The detection cell is one of the core components of the fluorescence detector, and the design of the detection cell directly affects the performance of the entire detection system. Researchers usually design the detection cell according to the designed excitation and emission light path structure. There are generally three ways to focus or collect the exc...

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