Liquid core waveguide fluorescence detector

A technology of fluorescence detection and liquid core waveguide, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of interference of detection results, etc., and achieve the effects of low cost, improved signal-to-noise ratio, and wide application range

Inactive Publication Date: 2013-07-03
常州博世伟业生物科技有限公司
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Problems solved by technology

The excitation light and fluorescence signals of this type of liquid core waveguide technology are transmitted from one end together for collection and detection, but the separation of excitation light and fluorescence cannot be achieved well, and there is great interference to the detection results

Method used

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specific Embodiment 1

[0028] Such as figure 1 As shown, the liquid-core optical fiber 8 uses a Teflon AF2400-coated quartz capillary with a specification of 50 μm ID×45 / 70 cm (separation length / total length). The excitation light source 1 is a laser, and the liquid core optical fiber 8 is composed of a coating layer 10, a quartz layer 11, and a liquid medium 12, and its refractive index increases sequentially. The shading plate 7 is a black plastic sheet with an inner diameter of 0.4 mm and a thickness of 0.5 mm. The shading plate 7 is a diaphragm in the filter system, and the optical filter 6 is a long-pass optical interference filter with a cut-off wavelength of 520 nm. One end of the liquid core fiber 8 removes a small piece of coating layer 10 as an incident window, and the excitation light ray 2 emitted by the excitation light source 1 enters the interior of the liquid core fiber 8 from the incident window at a certain angle. The excitation light ray 2 is totally reflected on the inner wall o...

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Abstract

The invention relates to a liquid core waveguide detector, in particular to a liquid core waveguide fluorescence detector, which comprises a liquid core optical fiber, an exciting light source, and a fluorescence detection component. The side wall of the liquid core optical fiber is provided with an incident window; exciting liquid emitted by the exciting light source is emitted into the liquid core optical fiber at a proper angle through the incident window on the side all of the liquid core optical fiber, and is spread in a total reflection way along the axis direction of the liquid core optical fiber; a test sample in a liquid medium in the liquid core optical fiber is excited and emits fluorescence; and the fluorescence detection component detects the fluorescence transmitted through the side wall of the liquid core optical fiber. The liquid core waveguide detector has the advantages that: the exciting light and the fluorescence are separated by a liquid core waveguide principle, and the background noise of the detector can be reduced effectively; the exciting light is spread in a capillary tube along the axis, the exciting optical path and utilization rate are greatly increased, and the sensitivity of the detector is improved; meanwhile, the device is simple in structure, convenient to use, low in cost and wide in application range.

Description

technical field [0001] The invention relates to a liquid core waveguide detection device, in particular to a liquid core waveguide fluorescence detection device. Background technique [0002] Liquid Core Waveguide (LCW) phenomenon, also known as liquid core fiber. It means that when the refractive index of the peripheral material is lower than that of the liquid core, and the incident angle of the incident light is greater than the critical incident angle, the light will be totally reflected at the interface between the liquid and the pipe wall, and the light within a certain angle will travel along the liquid Axial propagation of the core that conducts the output from the other end with little loss. [0003] The research on LCW first began in the middle of the 19th century. Colladon, Babinet, Tyndall and others studied the phenomenon of light propagating in stream water through total internal reflection. By the end of the 19th century, the phenomenon was widely known for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 张凌怡刘翻张权青
Owner 常州博世伟业生物科技有限公司
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