Light path device of fluid analysis equipment

Abstract

The invention discloses a light path device of fluid analysis equipment. The light path device comprises a laser irradiation unit, a fluid tank and a fluorescent light splitting unit, wherein a flowing fluid sample is borne in the fluid tank and contains a fluorescent dye labelled substance to be detected; the laser irradiation unit carries out laser irradiation on the fluid sample in the fluid tank and obtains the labelled fluorescent light through excitation; the fluorescent light is received by a fluorescent light receiver of the fluid analysis equipment after being split by the fluorescent light splitting unit; the fluorescent light splitting unit comprises a corner cube used for dispersing the fluorescent light into a plurality of fluorescent light beams; the corner cube is arranged between the fluid tank and the fluorescent light receiver of the fluid analysis equipment. The invention aims to provide the light path device used for the fluid analysis equipment such as flow cytometers. The light path device can reduce the light energy loss during fluorescent light splitting, improve the fluorescent light collection efficiency and enhance the capacity of processing weak signals of subsequent circuits.

Description

technical field [0001] The invention relates to an optical path device of fluid analysis equipment, in particular to an optical path device of a flow cytometer. Background technique [0002] Flow cytometer is a precision biomedical analysis instrument integrating optics, electronics, fluid mechanics, cytochemistry and computer technology, which is used for rapid quantitative analysis and sorting of cells. With the development of various technologies, flow cytometry has become an important tool for biological and medical research. Higher sensitivity, resolution and more excitation wavelengths are the goals of flow cytometer developers and researchers. combine figure 1 The schematic diagram of the optical path device structure of the flow cytometer shown in the prior art, the multiple lasers (such as 405nm, 488nm, 633nm, etc.) The beam mirror 20 implements multi-path laser synthesis, and focuses the laser beams inside the fluid pool 40 through a common focusing lens 30 . Be...

AI Technical Summary

Problems solved by technology

Because the same focusing lens is used when the laser beams are combined, resulting in a wide spectral range, it is necessary to achromatize the focusing optical system, and it is also difficult to achieve uniform focus spots and effective use of light energy

At present, the fluorescence collection optical system adopts the collection mirror 50 and the optical fiber and other light guide unit 60 for transmission. Although the layout of the follow-up optical path is flexible and convenient, the main problem is that the coupling efficiency of the fiber end is not high, resulting in weaker fluorescence signals. Detection of Weak Signals Brings Greater Challenges

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