Micro-nano system fluid chip detection system and detection method

Abstract

The utility model relates to a fluid chip detection system and detection method of micro-nano rise system, which is characterized in comprising an incident light generation system, a dual focal fluorescence collection system, a collecting light processing system, a circulation flowing stereo temperature control stage, and a microfluid chip; wherein the incident light generation system comprises at least a light source and a condenser arranged on the output of the light; the dual focal fluorescence collection system comprises a front imaging lens group and a rear imaging lens group and a detector arranged on the output of the post lens group; the collecting light processing system comprises an A / D acquisition card connected with the output of the detector, and a computer connected with the output of the A / D acquisition card; the temperature control stage comprises a motion platform bearing the microfluid chip, the heat transfer media around the motion platform, a heater arranged around the heat transfer media and a heat insulation device arranged outside the heater, the computer is connected with the motion platform through a motion controller, and connected with the heater and a temperature transmitter through a temperature feedback circuit. The utility model has the advantages of simple structure, low cost, high fluorescence efficiency collection and high detection sensitivity, convenient use and easy for promotion and popularization.

Description

technical field

[0001] The invention relates to a detection method and system of a microfluidic chip, in particular to a detection method and system of a micro-nanoliter system (1nL-10μL) fluid chip with low cost and relatively high detection sensitivity and resolution. Background technique

[0002] Microfluidic chip technology, also known as "Lab-on-a-chip", or micro-separation in Miniaturized Total Analysis System (μTAS: Micro-separation in Miniaturized Total Analysis System), is an emerging technology in the late 20th century. It is a multidisciplinary interdisciplinary technology, which uses system integration engineering technologies such as micro-electromechanical processing, computers, electronics, materials science, optics, mechanics, automatic control, software, etc. and multidisciplinary application fields such as analytical chemistry, biology, and medicine to carry out organic research. Cross-combination integrates discontinuous processes such as sample preparatio...

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