The invention provides a 
microscope objective optical 
system for 
video imaging, which is formed by sequentially arranging a first lens set, a second lens set, a light splitting 
prism, a third lens set, a fourth lens set, a fifth lens set, a sixth lens set, a seventh lens set, an eighth lens set and a ninth lens set along an 
optical axis from left to right, wherein a pre-positioned objective set of the 
system is formed by the first lens set and the second lens set; a main objective set of the 
system is formed by the third lens set, the fourth lens set, the fifth lens set, the sixth lens set, the seventh lens set, the eighth lens set and the ninth lens set; the 
focal length of the third lens se is fU3, the 
focal length of the fourth lens set is fU4 and the 
focal length of the main objective set is fE2; and a relation of the fU3, the fU4 and the fE2 is as follows: the absolute value of fU3 / fU4 is more than 0.46 and less than 0.68, and absolute value of fU3 / fE2 is more than 6.23 and less than 7.53. The 
microscope objective optical system disclosed by the invention has the characteristics of large-numerical-value hole 
diameter, large view field, long working distance, small relative 
distortion and high 
amplification factor in a waveband range of 360-550nm, and the 
imaging quality of the system reaches to the 
diffraction limit.