The application discloses a multi-channel molecular interaction detection device and method based on imaging and photoelectric array detection, and relates to the technical field of molecular interaction detection. The device comprises a multi-channel
optical fiber array, an imaging optical
system, a photoelectric detection unit and a
signal processing module. The imaging optical
system can perform spatial separation imaging on multiple light signals, or perform
time sequence separation imaging through a high-speed
shutter and channel
polling. The photoelectric detection unit adopts a
CMOS / CCD area sensor, a
photodiode array or a
photomultiplier tube array, so that a single detection module replaces traditional multiple spectrometers. The
signal processing module realizes non-
crosstalk signal extraction and analysis of each channel through ROI partition,
time sequence division or channel addressing. The application can be compatible with multiple signals such as biological layer interference spectrum,
laser reflection spectrum and up-conversion nanometer material probe
emission spectrum, has the advantages of low cost, small size, high channel consistency and strong expansibility, can effectively suppress channel
crosstalk, and is suitable for high-
throughput detection scenes.