Accurate and rapid organophosphorus pesticide detection method for vegetables

A technology of organophosphorus pesticides and detection methods, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of poor reproducibility of detection cycle detection, affecting consumers' food safety, and difficulty in storing vegetables for a long time, and shortening the grinding time. , The effect of less dosage, less solvent and sample dosage

Inactive Publication Date: 2019-07-12
ANHUI SANLIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the amount of pesticides remains high, and the unreasonable use of some pesticides makes the pesticide residues in agricultural products exceed the standard, which affects the food safety of consumers. The daily detection and risk monitoring of pesticide residues are becoming more and more important
With the improvement of people's living standards, people pay more and more attention to food safety issues. Vegetables are a kind of food that is closely related to people's lives. It is of great significance to detect their organophosphorus pesticide residues. In addition, because vegetables are not easy to store for a long time, if the detection If the cycle is too long and the detection reproducibility is poor, the detection significance will be greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] An accurate and rapid detection method for organophosphorus pesticides for vegetables, comprising the following steps:

[0028] S1. Preparation of standard sample solution: take phorate, methyl parathion, and malathion, and dilute them with n-hexane to a mass concentration of 0.2mg / kg, 0.5mg / kg, 1mg / kg, 5mg / kg, 10mg / kg, 50mg / kg standard sample solution;

[0029] S2. Mix 55 parts of acetone and 105 parts of cyclohexane uniformly by weight to obtain an eluent;

[0030] S3. Send 100 parts of vegetables and 60 parts of silicon dioxide into a grinder for grinding and crushing, the grinding pressure is 0.13 MPa, and the grinding time is 22 minutes to obtain a sample;

[0031] S4. Add 2.3 parts of anhydrous sodium sulfate, 2.5 parts of sample, and 2 parts of anhydrous sodium sulfate to the bottom of the syringe in parts by weight, compact it, use 10 parts of eluent to rinse and then flow out through the needle hole of the syringe, collect and wash Remove the liquid and place...

Embodiment 2

[0041] An accurate and rapid detection method for organophosphorus pesticides for vegetables, comprising the following steps:

[0042] S1. Preparation of standard sample solution: take phorate, methyl parathion, and malathion, and dilute them with n-hexane to a mass concentration of 0.2mg / kg, 0.5mg / kg, 1mg / kg, 5mg / kg, 10mg / kg, 50mg / kg standard sample solution;

[0043] S2. Mix 100 parts of acetone and 180 parts of cyclohexane uniformly by weight to obtain an eluent;

[0044] S3. Send 100 parts of vegetables and 78 parts of silicon dioxide into a grinder for grinding and crushing, the grinding pressure is 0.18 MPa, and the grinding time is 40 minutes to obtain a sample;

[0045] S4. Add 4 parts of anhydrous sodium sulfate, 3.8 parts of sample, and 3.5 parts of anhydrous sodium sulfate to the bottom of the syringe in parts by weight, compact, use 13.5 parts of eluent to rinse and then flow out through the needle hole of the syringe, collect the washed Remove the liquid and pla...

Embodiment 3

[0055] An accurate and rapid detection method for organophosphorus pesticides for vegetables, comprising the following steps:

[0056] S1. Preparation of standard sample solution: take phorate, methyl parathion, and malathion, and dilute them with n-hexane to a mass concentration of 0.2mg / kg, 0.5mg / kg, 1mg / kg, 5mg / kg, 10mg / kg, 50mg / kg standard sample solution;

[0057] S2. Mix 85 parts of acetone and 175 parts of cyclohexane uniformly by weight to obtain an eluent;

[0058] S3. Send 100 parts of vegetables and 75 parts of silicon dioxide into a grinder for grinding and crushing, the grinding pressure is 0.15 MPa, and the grinding time is 35 minutes to obtain a sample;

[0059] S4. Add 3 parts of anhydrous sodium sulfate, 3 parts of sample, and 3 parts of anhydrous sodium sulfate to the bottom of the syringe in parts by weight, compact, use 12 parts of eluent to rinse and then flow out through the needle hole of the syringe, collect the washed Remove the liquid and place it in ...

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Abstract

The invention discloses an accurate and rapid organophosphorus pesticide detection method for vegetables. The method comprises the following steps: preparing a standard sample solution; evenly mixingacetone and cyclohexane to obtain eluent; putting vegetables and silicon dioxide into a grinding machine to be ground and smashed with the grinding pressure being 0.12-1. 0.18 MPa and with the grinding time being 20-20 min. 40 minutes to obtain a sample; sequentially adding anhydrous sodium sulfate, a sample and anhydrous sodium sulfate to the bottom of the injector, compacting, leaching with theeluent, flowing out through a needle hole part of the injector, collecting the eluent, volatilizing the eluent in a ventilation cabinet until the eluent is dry, adding acetone for dissolving, and fixing the volume to obtain a to-be-detected solution; and analyzing by using a gas chromatography detector. The method disclosed by the invention is simple, high in detection sensitivity, high in reproducibility and high in detection speed.

Description

technical field [0001] The invention relates to the technical field of organophosphorus detection, in particular to an accurate and rapid detection method for organophosphorus pesticides used in vegetables. Background technique [0002] The development of modern agriculture has made the production of agricultural products more and more dependent on exogenous substances such as pesticides, antibiotics and hormones. Among them, the amount of pesticides remains high, and the unreasonable use of some pesticides makes the pesticide residues in agricultural products exceed the standard, affecting the food safety of consumers. The daily detection and risk monitoring of pesticide residues are becoming more and more important. With the improvement of people's living standards, people pay more and more attention to food safety issues. Vegetables are a kind of food that is closely related to people's lives. It is of great significance to detect their organophosphorus pesticide residues...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047G01N2030/062
Inventor 张世伟刘群
Owner ANHUI SANLIAN UNIV
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