Method for producing fucoxanthin by using mutant strain for expressing exogenous pyruvate phosphate dikinase in phaeodactylum tricornutum

A technology of pyruvate phosphate and Phaeodactylum, applied in the field of bioengineering, can solve the problem of low fucoxanthin production
CN112695047AActive Publication Date: 2021-04-23QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
6 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Publication Date
2021-04-23

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a method for producing fucoxanthin by using a mutant strain for expressing exogenous pyruvate phosphate dikinase in phaeodactylum tricornutum, and particularly relates to algae preference codon optimization on an original CyPPDK gene to obtain a new CyPPDK gene with a nucleotide sequence as shown in SEQ ID NO.7; the constructed shuttle expression vector is named as pPha-fcp-PPDK; and the obtained new gene is introduced into a host algae cell phaeodactylum tricornutum cell in an electric transformation manner, and a transformant is screened to obtain a phaeodactylum tricornutum transgenic algae strain for overexpression of pyruvate phosphate dikinase. Compared with an unoptimized transformant, the optimized transformant for phosphorylation sites of the CyPPDK gene has the advantages that the biomass accumulation is improved by 33.3%, the content of EPA in grease is improved by 11.5%, and the fucoxanthin yield is improved by 21.2%.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for producing fucoxanthin by expressing an overexpression mutant strain of exogenous pyruvate phosphate dikinase in Phaeodactylum tricornutum, belonging to the technical field of bioengineering. Background technique

[0002] Phaeodactylum tricornutum belongs to Diatomphyta, Featheria, Phaeodactylum, and Phaeodactylum genus. It is an important aquatic bait, rich in active substances such as EPA (Eicosapentaenoic Acid) and fucoxanthin (Fucoxanthin). Fucoxanthin, also known as fucoxanthin and fucoxanthin, is an oxygen-containing derivative of carotene, which has an allene bond, an epoxide and a conjugated carbonyl group on its molecular polyene chain group, which is a partial polar carotenoid. Fucoxanthin has a variety of biological activities. Taking fucoxanthin can speed up metabolism, increase the metabolism of triglyceride and cholesterol, and has pharmacological effects such as anti-inflammatory, anti-tumor, anti-o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More