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Method for culturing microalgae

A microalgae culture and microalgae technology, applied in the field of microalgae culture, can solve the problems of low biomass and slow growth rate, and achieve the effects of high growth rate, short cycle and reducing environmental pollution

Inactive Publication Date: 2010-12-22
ENERGY RES INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned method mainly adopts an autotrophic culture method, which has the disadvantages of slow growth rate and relatively low biomass.

Method used

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  • Method for culturing microalgae
  • Method for culturing microalgae

Examples

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Effect test

Embodiment 1

[0019] Embodiment 1: Step 1: Acidogenic fermentation. With 500g cow dung as the fermentation substrate, add water to adjust the solid content to 6%, fill it in the reaction device, inoculate 25% activated sludge (taken from the sewage treatment plant) and start fermentation, the temperature is controlled at 25 ± 1 ° C, the produced The gas is collected in the gas storage tank, and after 48 hours of fermentation, the fermentation product is subjected to solid-liquid separation through a screw extruder.

[0020] Step 2: Cultivation of algae species: the algae species used is chlorella vulgaris (purchased from Texas Algae Collection, UTEX 2714). The medium component is NH 4 Cl 400mg / L, KH 2 PO 4 56mg / L, K 2 HPO 4 108mg / L, MgSO 4 ·7H 2 O 100mg / L, glacial acetic acid 1mg / L, trace elements 1ml / L, the total oil content in this medium can reach more than 40% of the dry weight of the cells, and it is oil-rich microalgae. The algae has been experimentally confirmed to survive in...

Embodiment 2

[0022] Embodiment 2: The similarities between this embodiment and Example 1 will not be described in detail. The difference is that the autotrophic process can be continued after the heterotrophic culture. On the basis of Example 1, the microalgae after the heterotrophic culture are placed in In the tank-type photobioreactor, the mixed gas in the gas cylinder is circulated for autotrophic culture. The culture conditions are 28°C and 6000Lux light intensity. After 7 days, the biomass of microalgae is 18g / L, and the oil content is 37 %, hydrogen purity over 90%.

Embodiment 3

[0023] Embodiment 3: Step 1: Acidogenic fermentation. Use 500g corn stalks as the fermentation substrate, mix it with 500g cow dung and fill it in the reaction device, add water to adjust the solid content to 8%, inoculate 25% activated sludge (taken from the biogas digester) and start fermentation, the temperature is controlled at 25 ± 1°C, the generated gas was collected in the gas storage tank, and after 72 hours of fermentation, the fermentation product was separated from solid and liquid by a screw extruder. The rest of the steps are the same as step 2 in the embodiment. After 5 days, the biomass is 18g / L and the oil content is 39%.

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Abstract

The invention relates to a method for culturing microalgae, which is characterized by comprising the following steps of: (1) fermenting to produce acid, enabling the supernatant which is acid liquor obtained after fermentation to flow to a culture device, and collecting the produced gas mixture in a gas storage tank; (2) inoculating the cultured microalgae to the acid liquor contained in the culture device for heterotrophic culture; and (3) circularly introducing the gas mixture in the gas storage tank to a photobioreactor for aerobic autotrophic culture. In the invention, the acid production stage through methane fermentation and the microalgae culturing process are coupled, and heterotrophy and autotrophy are combined; thus, oil-rich microalgae with high biomass is cultured, and meanwhile, a hydrogen-rich gas with high concentration can be obtained. The method is mainly used for culturing the oil-rich microalgae.

Description

technical field [0001] The invention relates to microalgae cultivation technology. Background technique [0002] With the continuous aggravation of the global energy crisis and the continuous enhancement of people's awareness of environmental protection, renewable resources such as biodiesel have attracted more and more attention. Biodiesel is a fatty acid monoester obtained by transesterification of animal, vegetable or microbial oils and low-carbon alcohols. It is renewable and biodegradable, and its combustion and emission performance is better than that of fossil diesel. It is an environmentally friendly energy source. Gradually become a substitute for fossil diesel. Microalgae is a single-celled plant with the characteristics of high photosynthetic efficiency, strong environmental adaptability, short growth cycle and high biomass. In recent years, due to the development of biodiesel industry, the comprehensive development and utilization of oil-rich microalgae with hi...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12P5/02C12P3/00C12N1/12C02F11/04C12R1/89
Inventor 周文广李岩张晓东
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
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