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Method used for rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells

A fucoxanthin and non-destructive testing technology, which is applied in the direction of measuring devices, instruments, and biological particle analysis, can solve the problems of long analysis process time, fucoxanthin production process and quality control time-consuming and labor-intensive problems, and achieve simplified detection steps , shorten detection time, and simple sample pretreatment

Inactive Publication Date: 2018-07-13
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

The process of drying, extracting and analyzing samples by HPLC takes a long time, and it is time-consuming and labor-intensive for the production process and quality control of fucoxanthin
Usually, xanthin samples contain a large amount of foreign pigments, and the pretreatment method of UV method is too simple, and no corresponding pretreatment measures are taken for the interference of foreign pigments, so there are great limitations

Method used

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  • Method used for rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells
  • Method used for rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells
  • Method used for rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells

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Embodiment Construction

[0044] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention are described in detail below.

[0045] Flow cytometry (Flow Cytometry) can excite the fucoxanthin in the cells of Phaeodactylum tricornutum to emit fluorescence at 488nm, and detect the emission wavelengths of Phaeodactylum tricornutum cells at 533nm (FL1 channel) and 585nm (FL2 channel) respectively. mean fluorescence intensity. The inventors of the present application found that there is a high positive correlation between the average fluorescence intensity at the two specific emission wavelengths and the intracellular fucoxanthin content of Phaeodactylum tricornutum. By measuring the average fluorescence intensity and matching it with the HPLC measurement results, the accumulation of fucoxanthin can be accurately, quickly and non-destructively reflected. Compared with conventional methods, this method can greatly improve the ...

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Abstract

The invention relates to a method used for rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells. The method is capable of realizing rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells, and simplifying detection steps greatly; after establishment of a standard curve, extraction operation of samples is not needed in sample detection, sample pretreatment is simple, detection time is shortened, and at the same time, the method is especially suitable to be used for rapid nondestructive detection of fucoxanthin in microalgae cells, especiallyin Phaeodactylum tricornutum cells.

Description

technical field [0001] The invention belongs to the rapid non-destructive detection technology of intracellular pigments in microalgae, in particular to a method for rapid non-destructive detection of fucoxanthin in Phaeodactylum tricornutum cells based on fluorescence detection technology. Background technique [0002] Fucoxanthin is a carotenoid involved in photosynthesis, accounting for more than 10% of the total carotenoids in nature, and widely exists in algae such as brown algae, diatoms and golden algae. Fucoxanthin combines with chlorophyll and protein to form an FCP complex in algae cells, and plays an important role in physiological functions such as light harvesting, light energy transfer, and light protection in photosynthesis. Fucoxanthin has a unique allene structure and 5,6-epoxy structure (chemical formula as follows), which is different from other common carotenoids (such as β-carotene, lycopene, astaxanthin, etc.). [0003] [0004] Studies have proved ...

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Application Information

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IPC IPC(8): G01N15/14G01N15/10G01N15/00
CPCG01N15/00G01N15/10G01N15/1404G01N15/1434G01N2015/1006G01N15/01G01N15/1409
Inventor 魏东宋培钦杨润青
Owner SOUTH CHINA UNIV OF TECH
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