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Traingular brown algae open culture method and its special culture meidum

A cultivation method and technology of Phaeodactylum, applied in the field of open cultivation method of Phaeodactylum tricornutum and its special medium, can solve the problems of cultivation failure, easy pollution of single-celled green algae, incapable of large-scale cultivation, etc., and achieve prevention and control Effects of Chlorella Pollution

Inactive Publication Date: 2008-07-09
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

[0003] Phaeodactylum tricornutum in natural seawater grows mixed with other microalgae, and the population density is very low, which cannot meet the needs of the aquaculture industry
At present, the nutrient composition of most artificial mediums of Phaeodactylum tricornutum is suitable for the growth of green algae. Therefore, during the cultivation process of Phaeodactylum tricornutum, pollution of single-celled green algae such as Chlorella and salina often occurs. Cellular green algae grows fast, while consuming a large amount of nutrients in the medium, they also secrete extracellular products to inhibit the growth of Phaeodactylum tricornutum, which can easily lead to culture failure
At present, f / 2 medium is often used as medium for Phaeodactylum tricornutum, but this medium can only be cultivated in a small volume (about 1 liter) under laboratory conditions, and it is very easy to contaminate single-celled green algae, making large-scale cultivation impossible

Method used

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  • Traingular brown algae open culture method and its special culture meidum
  • Traingular brown algae open culture method and its special culture meidum
  • Traingular brown algae open culture method and its special culture meidum

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1, the growth cultivation of Phaeodactylum tricornutum in ZBPT medium

[0016] 1. Preparation of ZBPT medium

[0017] 1) Take 1L of natural static seawater, add 1N hydrochloric acid to adjust the pH of the seawater to 3, let it stand for 12 hours, and remove the sediment; then add 1N NaOH to adjust the pH to about 7.5, and let it stand to remove the sediment.

[0018] 2) Quantitatively weigh the following substances to prepare ZBPT medium: sodium nitrate (NaNO 3 ): 148mg; Potassium dihydrogen phosphate (KH 2 PO 4 ), 34.8 mg; sodium silicate (NaSiO 3 ), 238 mg; sodium bicarbonate (NaHCO 3 ), 450mg; f / 2 trace element solution, 1ml; improved f / 2 vitamin solution, 1ml; potassium iodide (KI), 800mg; step 1) dilute seawater to 1000ml. Among them, the f / 2 trace element solution is: zinc sulfate, 23mg; cobalt chloride, 12mg; manganese chloride, 178mg; copper sulfate, 10mg; sodium molybdate, 7.3mg; iron citrate, 3.9g; Disodium acetate, 4.35g; dilute to 1000ml wi...

Embodiment 2

[0023] Embodiment 2, the control effect of the ZBPT medium containing potassium iodide on the pollution of green algae in the cultivation process of Phaeodactylum tricornutum

[0024] Prepare ZBPT medium according to the following formula: sodium nitrate, 135mg; potassium dihydrogen phosphate, 38mg; sodium silicate, 215mg; sodium bicarbonate, 500mg; f / 2 trace element solution, 1ml; improved f / 2 vitamin solution, 1ml ; Set the volume of seawater to 1000ml.

[0025] Some of them were added with 800mg / L potassium iodide, and the other part was not added. Phaeodactylum tricornutum was aerated and cultured for 12 days at a temperature of 25° C., a light time of 8 h / day, an inoculum size of 1:9, and a light intensity of 5000 Lux. Other operations were the same as in Example 1. The growth curve of Phaeodactylum tricornutum figure 2 As shown, A in the figure is without adding potassium iodide; B is adding 800mg / L potassium iodide.

[0026] The results showed that when Phaeodactylu...

Embodiment 3

[0027] Embodiment 3, growth and cultivation of Phaeodactylum tricornutum in different potassium iodide concentration ZBPT medium

[0028] Prepare ZBPT medium according to the following formula: sodium nitrate, 165mg; potassium dihydrogen phosphate, 32mg; sodium silicate, 255mg; sodium bicarbonate, 400mg; f / 2 trace element solution, 1ml; improved f / 2 vitamin solution, 1ml ; Set the volume of seawater to 1000ml.

[0029] Add different concentrations of potassium iodide to it: 0, 200, 400, 600, 800, 1000, 1200, 1400mg / L, at a temperature of 25°C, a light time of 12h / day, an inoculum size of 1:9, and a light intensity of 5000Lux Under the conditions, aerated and cultivated Phaeodactylum tricornutum for 12 days, other operations were the same as in Example 1. The growth curve of Phaeodactylum tricornutum image 3 As shown, the A-H potassium iodide concentrations in the figure are 0, 200, 400, 600, 800, 1000, 1200, 1400mg / L respectively.

[0030] It can be seen from the figure th...

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Abstract

This invention discloses the Phaeodactylum tricornutum trained method and special-purpose Culture medium openly. This invention offers Culture medium is named ZBPT, its prescription is: Sodium nitrate, 135-165mg; potassium dihydrogen phosphate, 32-38mg; Sodium silicate , 215-255mg; Sodium bicarbonate , 400-500mg; KI, 400-1000mg; The trace element solution of f / 2, 1ml; Improved vitamin solution of f / 2, 1ml; The sea water holds to 1000ml definitely. Including the following step: 1)Dispose the culture medium with the sea water; 2)Phaeodactylum tricornutum density is 3*l05 cell / milliliter, according to make trigonometry brown to mean for1:5-9's proportions algae inoculate reach the culture compared with volume of culture, in 19-25 DEG C, illumination intensity 3000-6000 Lux, illumination time is 8-12 h / day terms keeps in touch with the medium.

Description

technical field [0001] The invention relates to a method for cultivating algae and a special culture medium thereof, in particular to an open culture method for Phaeodactylum tricornutum and its special culture medium. Background technique [0002] Phaeodactylum tricornutum (Phaedactylum tricornutum) belongs to the class of diatoms and is a primary productivity algae in common marine ecosystems. Phaeodactylum tricornutum is rich in a variety of unsaturated fatty acids, especially eicosapentaenoic acid (EPA), making it a delicious bait for many precious aquatic animals and a raw material for marine biopharmaceuticals. [0003] Phaeodactylum tricornutum in natural seawater grows mixed with other microalgae, and the population density is very low, which cannot meet the needs of the aquaculture industry. At present, the nutrient composition of most artificial mediums of Phaeodactylum tricornutum is suitable for the growth of green algae. Therefore, during the cultivation proces...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12A01G33/00C12R1/89
CPCY02A40/80
Inventor 郑维发鲍康德
Owner XUZHOU NORMAL UNIVERSITY
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