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Coextraction method for phaeodactylum tricornutum fucoxanthine and polyunsaturated fatty acid

A technology for unsaturated fatty acids and Phaeodactylum fucoidans, which is applied in the field of bioengineering, can solve the problems of different physical and chemical properties, inapplicability, etc., and achieves the effects of easy recovery, cost control, and easy acquisition.

Active Publication Date: 2019-11-15
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the paper "Research on the Process of Extracting Tea Seed Oil and Tea Saponin by n-Hexane-Water-Ethanol Two-liquid Phase System" (Lv Qi, 2017-05-01, "Jiangxi University of Science and Technology"), n-hexane-ethanol double The process of extracting tea seed oil and tea saponin in liquid phase system, but the physical and chemical properties of fucoxanthin and EPA are different from those in the literature. Therefore, the process method in the literature is not suitable for triangular brown Extraction of Fucoxanthin and Unsaturated Fatty Acids from Dactylobacter algae

Method used

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  • Coextraction method for phaeodactylum tricornutum fucoxanthine and polyunsaturated fatty acid
  • Coextraction method for phaeodactylum tricornutum fucoxanthine and polyunsaturated fatty acid
  • Coextraction method for phaeodactylum tricornutum fucoxanthine and polyunsaturated fatty acid

Examples

Experimental program
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Effect test

Embodiment 1

[0058] A method for co-extracting Phaeodactylum tricornutum fucoxanthin and polyunsaturated fatty acids, comprising the steps of:

[0059] 1. Take a small amount of the harvested algae mud and weigh it. After calculating the weight, put the wet algae mud into an oven at 105°C for 12 hours. After drying, calculate the weight after drying. Calculate the water content of the wet algae mud according to the weight difference before and after.

[0060] 2. When the water content of the wet algae mud does not exceed 85%, add absolute ethanol to the wet algae mud. The mass volume ratio of the wet algae mud to absolute ethanol is 1:3, and the unit is g / ml.

[0061] 3. Stir the mixed solution at 45°C with an automatic stirrer, and keep away from light during the whole process.

[0062] 4. After stirring and extracting for 3 hours, use a suction filtration device to separate the solid algae residue from the extract, and use a polypropylene filter membrane with a pore size of 10 μm for su...

Embodiment 2

[0074] Example 2 is basically the same as Example 1, except that the extraction temperatures used in step 3 and step 6 are 24°C, 37°C, 45°C and 60°C, respectively.

[0075] It has been tested that the extraction rate of fucoxanthin is different at different temperatures, and the extraction efficiency is the highest at 45°C. The extraction efficiency of EPA is also different at different temperatures, and the extraction efficiency of EPA is the highest at 37°C and 45°C. Based on the above, 45°C is the optimal temperature for extraction ( image 3 ).

Embodiment 3

[0077] Embodiment 3 is basically the same as Example 1, the difference being that the ratio of ethanol and normal hexane used in step 5 is different, when the quality of algae cake 1 obtained in step 5 is 1, use different volume ratios of ethanol and normal hexane to carry out Extraction is respectively ethanol:n-hexane=1:3; ethanol:n-hexane=1:2; ethanol:n-hexane=1:1; ethanol:n-hexane=1:0.5.

[0078] After testing, different solvent ratios will also affect the extraction efficiency, mainly affecting the extraction of oil. Under the condition of ethanol:n-hexane=1:2 or 1:3, the extraction efficiency of oil is the highest, based on economical efficiency Considering, use ethanol: the condition of normal hexane=1:2 as optimal oil extraction condition ( Figure 4 ).

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Abstract

The invention relates to a coextraction method for phaeodactylum tricornutum fucoxanthine and a polyunsaturated fatty acid. The coextraction method comprises the following steps: (1) taking wet alga mud, adding ethanol, performing stirring extraction so as to obtain a mixed liquid of the wet alga mud and the ethanol, and performing solid-liquid separation so as to obtain a pre-extraction liquid and alga cakes 1; (2) mixing the algae cakes 1 with ethanol and normal hexane, performing stirring extraction so as to obtain a double-liquid phase mixed liquid, and performing solid-liquid separation so as to obtain a double-liquid phase extraction liquid and filter cakes 2; and (3) mixing the pre-extraction liquid with the double-liquid phase extraction liquid so as to obtain a mixed liquid, performing layering treatment so as to obtain an ethanol phase and a normal hexane phase, drying the ethanol phase so as to obtain a fucoxanthine extract, and drying the normal hexane phase, so as to obtain a polyunsaturated fatty acid extract. By adopting the method provided by the invention, the fucoxanthine and the polyunsaturated fatty acid can be effectively coextracted, and phaeodactylum tricornutum can be in best use.

Description

technical field [0001] The invention relates to a co-extraction method of Phaeodactylum tricornutum fucoxanthin and polyunsaturated fatty acid, belonging to the technical field of bioengineering. Background technique [0002] Fucoxanthin, also known as fucoxanthin or fucoxanthin, is an oxygen-containing derivative of carotenoids and is commonly found in brown algae and diatoms. Fucoxanthin functions as a light-harvesting pigment in algal cells. Fucoxanthin, chlorophyll a and chlorophyll c form fucoxanthin-chlorophyll protein complex (Fucoxanthin-Chlorophyll Protein, FCP), which is an important photosynthetic system element in diatoms. Fucoxanthin has been proven to be a safe and effective dietary supplement with various physiological activities such as anti-obesity, anti-diabetes, anti-oxidation, anti-inflammation, and anti-tumor [1]. [0003] Fucoxanthin is a kind of fat-soluble pigment, which is hardly soluble in water and other highly polar solvents under neutral condit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D301/32C07D303/32C07C51/42C07C57/03
CPCC07C51/42C07D301/32C07D303/32C07C57/03
Inventor 范勇李福利丁晓婷王丽娟胡光荣
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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