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Method for extracting fucoxanthin from phaeodactylum tricornutum with dimethyl ether fluid

A technology of Phaeodactylum tricornutum fucoxanthin and dimethyl ether, which is applied in the field of microalgae cultivation, can solve the problems that do not involve the extraction of Phaeodactylum tricornutum fucoxanthin, the destruction of Phaeodactylum tricornutum fucoxanthin, the extraction solvent and High energy consumption and other problems, to achieve the effect of reliability and safety, reduce energy consumption, and reduce consumption

Active Publication Date: 2018-06-08
YUNNAN AIERKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These four patents all use subcritical fluid dimethyl ether as the extraction agent, and the process methods are similar, only the materials are different, but none of them involve the extraction of fucoxanthin from Phaeodactylum tricornutum
[0010] In the production practice of extracting fucoxanthin from Phaeodactylum tricornutum, the water content of the fresh algae of Phaeodactylum tricornutum usually exceeds 60%, and various drying and dehydration methods such as thermal processing, electromagnetic processing, and vacuum processing are used, all of which are harmful to the fucoxanthin of Phaeodactylum tricornutum. The siliceous shell of Phaeodactylum tricornutum is relatively tightly combined, and it is difficult for organic solvents to penetrate into the cytoplasm effectively to contact with liposomes such as pigments, and the extraction efficiency of fucoxanthin is low; Phaeodactylum tricornutum fucoxanthin The element content is about 1.0%, the concentration of the extract is quite low, and the extraction solvent and energy consumption remain high
The general subcritical extraction method, the static immersion extraction is also difficult to meet the requirements

Method used

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  • Method for extracting fucoxanthin from phaeodactylum tricornutum with dimethyl ether fluid
  • Method for extracting fucoxanthin from phaeodactylum tricornutum with dimethyl ether fluid
  • Method for extracting fucoxanthin from phaeodactylum tricornutum with dimethyl ether fluid

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Embodiment 1

[0050] A method for extracting fucoxanthin from Phaeodactylum tricornutum with dimethyl ether fluid, comprising steps:

[0051] 1) Freezing and thawing fresh algae of Phaeodactylum tricornutum;

[0052] 2) Dimethyl ether fluid dynamic gradient countercurrent extraction of broken algae mud;

[0053] 3) The extract solution is precipitated to separate the fucoxanthin.

[0054] The fresh algae of Phaeodactylum tricornutum in the step 1) is the fresh algae of Phaeodactylum tricornutum dried by a high-speed centrifuge, and the water content mass percentage is 65.0%.

[0055] The fresh algae of Phaeodactylum tricornutum are first quick-frozen, then refrigerated, and finally thawed to achieve freeze-thaw wall breaking. The quick-freezing temperature is -70°C, the quick-freezing time is 8.0h, the cold storage temperature is -20°C, the thawing temperature is 20°C, and the thawing time is is 0.5, and the number of freeze-thaw cycles is 1 time.

[0056] The dimethyl ether fluid in the...

Embodiment 2

[0063] A method for extracting fucoxanthin from Phaeodactylum tricornutum with dimethyl ether fluid, comprising steps:

[0064] 1) Freezing and thawing fresh algae of Phaeodactylum tricornutum;

[0065] 2) Dimethyl ether fluid dynamic gradient countercurrent extraction of broken algae mud;

[0066] 3) The extract solution is precipitated to separate the fucoxanthin.

[0067] The fresh algae of Phaeodactylum tricornutum in the step 1) is the fresh algae of Phaeodactylum tricornutum dried by a high-speed centrifuge, and the water content mass percentage is 70.0%.

[0068] The fresh algae of Phaeodactylum tricornutum are first quick-frozen, then refrigerated, and finally thawed to achieve freeze-thaw breaking. The quick-freezing temperature is -55°C, the quick-freezing time is 102.0h, the cold storage temperature is -15°C, the thawing temperature is 25°C, and the thawing time 1.0h, freeze-thaw times 23 times.

[0069] The dimethyl ether fluid in the step 2) is a subcritical fl...

Embodiment 3

[0076] A method for extracting fucoxanthin from Phaeodactylum tricornutum with dimethyl ether fluid, comprising steps:

[0077] 1) Freezing and thawing fresh algae of Phaeodactylum tricornutum;

[0078] 2) Dimethyl ether fluid dynamic gradient countercurrent extraction of broken algae mud;

[0079] 3) The extract solution is precipitated to separate the fucoxanthin.

[0080] The fresh algae of Phaeodactylum tricornutum in the step 1) is the fresh algae of Phaeodactylum tricornutum dried by a high-speed centrifuge, and the water content mass percentage is 5.0%.

[0081] The fresh algae of Phaeodactylum tricornutum are first quick-frozen, then refrigerated, and finally thawed to achieve freeze-thaw breaking. The quick-freezing temperature is -40°C, the quick-freezing time is 12.0h, the cold storage temperature is -10°C, the thawing temperature is 30°C, and the thawing time is 1.5h, freeze-thaw times 3 times.

[0082] The dimethyl ether fluid in the step 2) is a subcritical fl...

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Abstract

The invention discloses a method for extracting fucoxanthin from phaeodactylum tricornutum with a dimethyl ether fluid. The method comprises the steps as follows: 1) fresh phaeodactylum tricornutum issubjected to freezing and thawing wall breaking; 2) wall-broken alga paste is subjected to dynamic gradient countercurrent extraction with the dimethyl ether fluid; 3) an extraction solution is subjected to desolvation for separation of fucoxanthin. Damage to effective ingredients in microalgae due to dewatering with physical and chemical means can be effectively avoided, energy consumption of the extraction process can be substantially reduced, dynamic gradient countercurrent extraction operation is adopted, the concentration of effective ingredients of the extraction solution can be effectively increased, the dosage of an extraction agent can be decreased, the extraction efficiency can be improved, the fucoxanthin from phaeodactylum tricornutum can be extracted efficiently, and the method is reliable and safe.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a method for extracting fucoxanthin from Phaeodactylum tricornutum with dimethyl ether fluid. Background technique [0002] Fucoxanthin, also known as fucoidin, fucoxanthin, molecular formula C 42 h 58 o 6 , the structural formula is as follows, the molecular weight is 658.91, the density is 1.09, the melting point is 166-168°C, and the CAS registration number is 3351-86-8. Important carotenoids are substances that brown algae and are indicator pigments of diatom plants. [0003] [0004] Fucoxanthin has a unique structure and shows potential value advantages with promising application prospects in a variety of pharmacological activities, including anti-inflammatory, anti-tumor, anti-obesity, anti-oxidation, anti-acne, anti-diabetes, anti-malarial and anti-lipidemia and other physiological activities It has been partially confirmed, and other pot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32C07D303/32
Inventor 张吉和沈家会蒋子华李春华王兴勇张勇
Owner YUNNAN AIERKANG BIOTECH
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