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Method for cultivating phaeodactylum tricornutum bohlin by virtue of two-stage process with adoption of waste zip-top cans and narrow-mouthed bottle

A technology of Phaeodactylum and cans, which is applied in the field of aquaculture, can solve the problems of large floor area, large basic investment, and large power consumption, and achieve the effects of small floor area, low investment, and low power consumption

Inactive Publication Date: 2016-12-07
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Through dark cultivation, it saves energy, has no pollution, and no harmful substances are produced. It injects a booster into today's microalgae oil production industry, but the equipment is complicated, the basic investment is large, the area is large, and the power consumption is large.

Method used

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  • Method for cultivating phaeodactylum tricornutum bohlin by virtue of two-stage process with adoption of waste zip-top cans and narrow-mouthed bottle
  • Method for cultivating phaeodactylum tricornutum bohlin by virtue of two-stage process with adoption of waste zip-top cans and narrow-mouthed bottle

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Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0013] Embodiment 1 Phaeodactylum tricornutum culture solution is made up of the batching of following parts by weight: industrial ammonia water (containing ammonia 25%-28%) 2 parts, ammonium sulfate 0.2 part, superphosphate 0.06 part, sucrose 0.5 part, glucose 0.4 part , 0.07 parts of potassium silicate, 0.005 parts of copper sulfate, 0.03 parts of ferrous chloride, 0.001 parts of manganese gluconate, 0.003 parts of zinc acetate, 0.007 parts of potassium iodate, 0.002 parts of cobalt sulfate, 0.06 parts of sodium selenate, sodium molybdate 0.02 parts, vanadium pentoxide 0.006 parts, vitamin B1 0.004 parts, vitamin B12 0.0003 parts, vitamin H 0.0006 parts, folic acid 0.0005 parts, inositol 0.006 parts, compound Chinese herbal liquid 4 parts, ethephon 0.00004 parts, boiled and cooled sea water 1000 parts share. Wherein the preparation method of the compound Chinese herbal medicine liquid: take 5 parts of Luo Han Guo, 1 part of loquat fruit, 4 parts of bellflower, 5 parts of Pan...

Embodiment 2 3

[0014] Embodiment 2 The Phaeodactylum tricornutum culture solution is composed of the following ingredients in parts by weight: 3 parts of industrial ammonia (containing 25%-28% of ammonia), 0.3 part of ammonium sulfate, 0.07 part of superphosphate, 0.6 part of sucrose, and 0.4 part of glucose , 0.08 parts of potassium silicate, 0.003 parts of copper sulfate, 0.04 parts of ferrous chloride, 0.002 parts of manganese gluconate, 0.005 parts of zinc acetate, 0.006 parts of potassium iodate, 0.003 parts of cobalt sulfate, 0.07 parts of sodium selenate, sodium molybdate 0.03 parts, vanadium pentoxide 0.007 parts, vitamin B1 0.005 parts, vitamin B12 0.0005 parts, vitamin H 0.0008 parts, folic acid 0.0007 parts, inositol 0.006 parts, compound Chinese herbal liquid 5 parts, ethephon 0.00007 parts, boiled and cooled sea water 1000 parts share. Wherein the preparation method of compound Chinese herbal medicinal liquid: take 7 parts of Luo Han Guo, 2 parts of loquat fruit, 5 parts of bell...

Embodiment 3 3

[0015]Embodiment 3 Phaeodactylum tricornutum culture solution is made up of the batching of following parts by weight: 3 parts of industrial ammonia water (containing ammonia 25%-28%), 0.4 part of ammonium sulfate, 0.08 part of calcium superphosphate, 0.6 part of sucrose, 0.5 part of glucose , 0.09 parts of potassium silicate, 0.006 parts of copper sulfate, 0.06 parts of ferrous chloride, 0.003 parts of manganese gluconate, 0.007 parts of zinc acetate, 0.008 parts of potassium iodate, 0.003 parts of cobalt sulfate, 0.09 parts of sodium selenate, sodium molybdate 0.04 parts, vanadium pentoxide 0.008 parts, vitamin B1 0.004 parts, vitamin B12 0.0008 parts, vitamin H 0.0007 parts, folic acid 0.0005 parts, inositol 0.008 parts, compound Chinese herbal medicine liquid 7 parts, ethephon 0.00007 parts, boiled and cooled sea water 1000 parts share. Wherein the preparation method of the compound Chinese herbal medicine liquid: get 4 parts of Luo Han Guo, 3 parts of loquat fruit, 7 part...

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Abstract

The invention discloses a method for cultivating phaeodactylum tricornutum bohlin by virtue of a two-stage process with adoption of waste zip-top cans and a narrow-mouthed bottle, and belongs to the field of aquaculture. In the past, the phaeodactylum tricornutum bohlin, which is always cultivated by virtue of an open raceway pond, has the problems of being high in basic investment, easily polluted, difficult to scrub, inflexible to operate, large in occupied space, greatly affected by seasons in production and unstable. In order to overcome shortcomings of the conventional technology, the invention provides the method for cultivating the phaeodactylum tricornutum bohlin by virtue of the two-stage process with adoption of the waste zip-top cans and the narrow-mouthed bottle; the prescription of a disclosed phaeodactylum tricornutum bohlin nutrient solution is more scientific and reasonable, and the cultivated phaeodactylum tricornutum bohlin is rapid in growth, small in occupied space, low in investment, low in cost, easy to operate, not easily polluted, convenient to scrub and not limited by the seasons in production; the contents of polyunsaturated fatty acids EPA and DHA in phaeodactylum tricornutum bohlin cells are high, electric energy consumption is low and yield is improved by 45%.

Description

[0001] Technical field The present invention belongs to the field of aquaculture, and in particular relates to a two-step method for cultivating Phaeodactylum tricornutum by using waste cans and narrow-mouth bottles. Background technique [0002] Phaeodactylum tricornutum Bohlin belongs to Diatomphyta, Featheridae, Phaeodactylaceae, Phaeodactyceae, Phaeodactylaceae. It has important economic value because of its high polyunsaturated fatty acid content. It can be used as clam, Oyster, cockle, sea urchin, sea cucumber bait. Regarding the cultivation method of Phaeodactylum tricornutum, in the past Zheng Weifa et al disclosed an open cultivation method of Phaeodactylum tricornutum and its special medium (application number CN200410047884.2). The culture medium provided by this invention is named ZBPT, and its formula is: sodium nitrate, 135-165 mg; potassium dihydrogen phosphate, 32-38 mg; sodium silicate, 215-255 mg; sodium bicarbonate, 400-500 mg; -1000mg; f / 2 trace element so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12M1/24C12R1/89
CPCC12M21/02C12M23/08C12N1/12
Inventor 王培磊徐树建刘笑天刘金民陈玉倩
Owner LINYI UNIVERSITY
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