The invention discloses a method for cultivating 
phaeodactylum tricornutum bohlin by virtue of a two-stage process with adoption of waste zip-top cans and a narrow-mouthed 
bottle, and belongs to the field of 
aquaculture. In the past, the 
phaeodactylum tricornutum bohlin, which is always cultivated by virtue of an open 
raceway pond, has the problems of being high in basic investment, easily polluted, difficult to scrub, inflexible to operate, large in occupied space, greatly affected by seasons in production and unstable. In order to overcome shortcomings of the conventional technology, the invention provides the method for cultivating the 
phaeodactylum tricornutum bohlin by virtue of the two-stage process with adoption of the waste zip-top cans and the narrow-mouthed 
bottle; the prescription of a disclosed phaeodactylum tricornutum bohlin 
nutrient solution is more scientific and reasonable, and the cultivated phaeodactylum tricornutum bohlin is rapid in growth, small in occupied space, low in investment, low in cost, easy to operate, not easily polluted, convenient to scrub and not limited by the seasons in production; the contents of polyunsaturated fatty acids EPA and DHA in phaeodactylum tricornutum bohlin cells are high, 
electric energy consumption is low and yield is improved by 45%.