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Method for extracting biological protein from aurantiochytrium sp residue

A technology of Schizochytrium and protein, applied in animal feed, animal feed, application, etc., can solve the problem that it is difficult to perfectly replace fish meal, achieve comprehensive utilization and increase economic benefits

Inactive Publication Date: 2013-05-01
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many studies have been carried out on the use of plant protein resources to replace fish meal, it is difficult to achieve the effect of replacing fish meal perfectly, mainly for the following reasons: the balance of amino acid composition (fish meal contains high levels of essential amino acids and sulfur-containing amino acids), protein Bioavailability (amino acids in fish meal are mainly in the form of small peptides that are very easy to digest and absorb), essential polyunsaturated fatty acid content (fish meal contains more polyunsaturated fatty acids, such as docosahexaenoic acid DHA content 0.03%~0.9%) etc.

Method used

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  • Method for extracting biological protein from aurantiochytrium sp residue

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preparation example Construction

[0027] Preparation of secondary seed liquid: Put the above-mentioned primary seed liquid into a 500ml shake flask containing 100ml seed culture medium, in a shaker at 20~30℃, rotate at 150~200rpm, cultivate for 24~48h to obtain secondary For the seed liquid, the above-mentioned seed medium and the first-level seed liquid use the same medium.

[0028] Connect the secondary seed liquid into a fermenter equipped with fermentation medium, the inoculum amount is 2-10%v / v, the aeration rate is 0.2-2vvm, the rotating speed is 200-800rpm, the tank temperature is 20-30℃, and the pH is 6-7. Schizochytrium oil, the above fermentation medium includes the following components: glucose 20-60g / L, yeast extract 5-30g / L, peptone 5-20g / L, potassium dihydrogen phosphate 0.5-8g / L, magnesium sulfate 0.5~5g / L, sodium citrate 0.5~5g / L, sea crystal 5~30g / L, vitamin B110~100mg / L, vitamin B610~100mg / L, vitamin B123~50mg / L, biotin 2~50mg / L. The above fermentation method can be batch fermentation, fed-bat...

Embodiment 1

[0031] Centrifuge the above fermentation broth, wash the insoluble matter twice, collect the insoluble matter algae, add an appropriate amount of water to adjust the viscosity, perform high-pressure homogenization and break the wall at a pressure of 40Mpa, and cycle twice;

[0032] Add n-hexane and ethanol to extract 3 times, and the organic phase is evaporated to dry the solvent to obtain crude oil. After alkali refining, decolorization and deodorization, 562 grams of polyunsaturated fatty acid refined oil is obtained, containing 38.5% of DHA;

[0033] Add 1% pectinase to the remaining algae residue, hydrolyze at 50°C for 3 hours, then add 1% protease, temperature 50°C, action time 3 hours, spray drying, inlet air temperature 160°C, outlet air temperature 80°C, get dry powder about 350 G, protein content is about 56.5%, DHA content is 0.02%, it can be used as protein feed additive.

Embodiment 2

[0035] Centrifuge the fermentation broth, wash the insoluble matter twice, collect the insoluble matter algae, add appropriate amount of water to adjust the viscosity, and break the wall by enzymatic method;

[0036] Add n-hexane and ethanol to extract 3 times, evaporate the organic phase to dry the solvent to obtain crude oil. After alkali refining, decolorization and deodorization, 551 grams of polyunsaturated fatty acid refined oil is obtained, containing 38.3% DHA;

[0037] Add 0.1% pectinase to the remaining algae residue, hydrolyze at 60°C for 5 hours, then add 0.1% protease, hydrolyze at 60°C for 5 hours, spray dry, inlet air temperature 190°C, outlet air temperature 90°C, get dry powder about 354 grams, protein The content is about 56.9%, and the DHA content is 0.05%. It can be used as a protein feed additive.

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Abstract

The invention provides a method for extracting a biological protein from an aurantiochytrium sp residue. The method comprises the following steps: performing fermentation culture on an aurantiochytrium sp strain, separating obtained fermentation broth, collecting fronds, washing the collected fronds, and performing oil extraction to obtain crude oil; and alkali-refining, decolorizing and deodorizing the prepared crude oil to obtain aurantiochytrium sp oil and an aurantiochytrium sp residue, wherein the protein content of the aurantiochytrium sp residue is up to more than 50% through secondary enzymolysis, most of the protein exists in the form of small peptide capable of being easily digested, and the content of DHA (Docosahexaenoic Acid) in a polyunsaturated fatty acid is 0.02%-2%, so that the aurantiochytrium sp residue is a good fish meal protein feed additive. According to the method for extracting the biological protein from the aurantiochytrium sp residue, the waste aurantiochytrium sp residue obtained after extracting the aurantiochytrium sp oil is used fully so as to recycle the waste and improve the economic benefit; the comprehensive utilization of microalgae cells is realized; and the cleaner production of an unsaturated fatty acid is realized.

Description

Technical field [0001] The invention relates to a method for extracting biological protein, in particular to a method for extracting biological protein from Schizochytrium spp. Background technique [0002] As one of the important feed ingredients, fish meal has high protein content, balanced nutrition, and is beneficial to the absorption and utilization of feeding animals; fish fat contains a large proportion of polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), Eicosapentaenoic acid (EPA) has a good promoting effect on the normal growth and development of feeding animal larvae; fish meal is also a good source of calcium, phosphorus, iodine, selenium and other minerals. In addition, fish meal also contains a variety of vitamins, so Fish meal is an excellent feed material that is widely used and difficult to replace. However, with the development of social economy and excessive plunder of resources, limited fishmeal resources are gradually exhausted, which has brou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/14
Inventor 李悦明崔球张希铭宋晓金徐建春高莽夏修峦
Owner QINGDAO LANGYATAI GRP
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