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A Rapid and Effective Method for Extracting Oil from Cottonseed in Early Development

An extraction method and cottonseed technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of little correlation between fatty acid accumulation and dynamic changes

Inactive Publication Date: 2017-10-27
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few reports on the dynamic changes of fatty acid accumulation and the correlation between fatty acids during the development of cottonseed

Method used

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  • A Rapid and Effective Method for Extracting Oil from Cottonseed in Early Development
  • A Rapid and Effective Method for Extracting Oil from Cottonseed in Early Development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1, Cottonseed Sampling in Early Development

[0017] Mark the cotton when it blooms, and mark the flowering date, select plants with the same flowering period, mark the flowers that bloom on the same day on the main inflorescence and the first branch inflorescence, and take cotton seeds at the early stage of development 5 and 10 days after flowering Cottonseed samples in each stage were tested three times for each sample.

Embodiment 2

[0018] Embodiment 2, the oil extraction of early stage cottonseed

[0019] Use tweezers to take out the cottonseeds from the bolls, gently peel off the shell of the cottonseeds, etc., take out the cottonseeds and collect them in a 50ml centrifuge tube, freeze them quickly in liquid nitrogen, and store them in a -80°C ultra-low temperature refrigerator.

[0020] Take out the frozen cotton kernel sample, grind it into powder with liquid nitrogen, take 10 μg and put it into a 1.5ml centrifuge tube, add 0.6ml of chloroform-methanol (volume ratio 2:1) mixed solvent, at 40 ℃, 200r / min After 4 hours of treatment on a shaker, centrifuge at 6000r / min for 10 minutes.

[0021] Weigh the weight of the 1.5ml centrifuge tube with constant weight, then absorb the lower oil phase layer into the centrifuge tube, evaporate to dryness on a rotary vacuum dryer at 25°C to constant weight, weigh, subtract the centrifuge tube, and calculate the oil yield up to 30%.

[0022] In the above centrifuge...

Embodiment 3

[0024] Embodiment 3, the GC-MS analysis of cottonseed fatty acid component

[0025] Agilent Technologies 7890A GC System was used for GC-MS analysis, chromatographic column HP-5MS19091S-436 (60m×250μm×0.25μm); injection volume 1μL, split injection method, split ratio 50:1; inlet temperature 250°C ; Carrier gas Ne, 20mL / min. Programmable temperature rise, keep at 150°C for 1min, then rise to 180°C at a rate of 10°C / min, keep for 5min, rise to 230°C at a rate of 3°C / min, keep for 15min, and then rise to 270°C, keep for 20min.

[0026] Gas chromatographic determination shows that, compared with C16:0, C18:0, C18:1, C18:2, C18:3 of mature cottonseed (the relative content is respectively: 25%, 2%, 15%, 57%, 0.2%) In contrast, the accumulation of C16:0, C18:0, and C18:3 fatty acid components in cottonseed was mainly concentrated in the early and middle stages of seed development; , the relative contents of C18:2 and C18:3 were: 34.94%, 21.86%, 7.45%, 14.51%, 15.87% respectively; ...

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Abstract

It is difficult to extract the oil of early-stage cottonseed, which is not conducive to the analysis of its fatty acid components. The present invention establishes an effective, simple and fast method for extracting oil from early-stage cottonseed (5-10 days after flowering). The method avoids the oxidation of polyunsaturated fatty acid components in oils caused by extraction methods such as high temperature and conventional rotary evaporation; the extracted oils can be directly used for the identification and relative content analysis of fatty acid components by GC‑MS, and through extraction The method of adding an internal standard to the oil solvent was used to quantitatively analyze the fatty acid components of cottonseed oil in the early development stage. The invention can effectively and rapidly extract the total oil in the cottonseed, and is convenient for analyzing the dynamic changes of the oil content in the cottonseed and the accumulation of main fatty acids in the early stage of cotton seed development.

Description

technical field [0001] The present invention generally relates to the field of vegetable oil chemistry and the extraction of plant seed oil; more specifically, the present invention relates to a method for effectively and rapidly extracting the total oil from cottonseed 5-10 days after flowering, and using it for fatty acid components Analysis of identification and content. Background technique [0002] Cotton is both the most important fiber crop and an important oil crop. The oil content in cottonseed after husking is 25% to 39%, which is the fifth largest source of vegetable oil in the world after soybean, palm, rapeseed and sunflower. In 2012, the output of cottonseed in my country was about 11.86 million tons, the output of cottonseed oil was 1.447 million tons, and the consumption of cottonseed oil reached 1.444 million tons, making it the fourth largest edible vegetable oil in my country. [0003] In mature cottonseed, the fatty acid components of its oil are mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06
Inventor 刘峰赵伊英孙杰
Owner SHIHEZI UNIVERSITY
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