Method for promoting accumulation of fucoxanthin and/or lipids in phaeodactylum tricornutum

A technology for the accumulation of fucoxanthin and lipids in algae, which is applied to single-celled algae, and the addition of compounds to stimulate growth. The effect of increasing the content and easy operation

Inactive Publication Date: 2015-12-09
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no published research report on the use of 2,4-epibrassinolide to promote the accumulation of fucoxanthin and / or lipids in Phaeodactylum tricornutum

Method used

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  • Method for promoting accumulation of fucoxanthin and/or lipids in phaeodactylum tricornutum
  • Method for promoting accumulation of fucoxanthin and/or lipids in phaeodactylum tricornutum
  • Method for promoting accumulation of fucoxanthin and/or lipids in phaeodactylum tricornutum

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Embodiment 1

[0036] A method for promoting the accumulation of fucoxanthin and / or lipids in Phaeodactylum tricornutum, after inoculating Phaeodactylum tricornutum in several growth stages into seawater culture solution at a volume ratio of 10%, adding 2,4-epibrassinosin Lactone to make the final concentration of 0.05-0.15mg / L, at a culture temperature of 20°C, a light intensity of 5000lx, a light cycle of 12h / 12h, fully shake the flask 3 times a day, and co-culture for 10d. Add the sterilized Ningbo University No. 3 mother solution to the sterilized seawater in a ratio of 1:1000 and shake it evenly. The formula of the Ningbo University No. 3 mother solution is: KNO 3 10g, KH 2 PO 4 1g, FeSO 4 ·7H 2 O0.25g, MnSO 4 0.025g, EDTANa 2 1g, VB 1 0.0006,dH 2 O100ml.

Embodiment 2

[0038] The relationship between different concentrations of EBR treatment and the cell number of Phaeodactylum tricornutum

[0039] Using OD 680 The light absorption value of Phaeodactylum tricornutum is used to measure the cell number of Phaeodactylum tricornutum per unit volume of culture medium, and the OD of Phaeodactylum tricornutum is regularly measured every day 680 value, and according to the regression equation, obtain the growth status of the Phaeodactylum tricornutum treated by different concentrations of EBR, and obtain the growth curve figure after utilizing different concentrations of EBR to process Phaeodactylum tricornutum, as figure 2 As shown, the results show that: Phaeodactylum tricornutum can grow within the concentration range of 0~0.2mg / L EBR, the overall growth is relatively fast within 1~6d, and it is in the logarithmic growth phase, and the algae cell density within 7~8d Little change, in the stable period, after the 8th day, the algae cell density ...

Embodiment 3

[0041] The relationship between different concentrations of EBR treatment and the biomass and specific growth rate of Phaeodactylum tricornutum

[0042] Phaeodactylum tricornutum was treated with different concentrations of EBR, and its biomass and specific growth rate were changed. The result is as image 3 Shown: When the EBR concentration is less than 0.05mg / L, the biomass and specific growth rate of Phaeodactylum tricornutum are positively correlated with the concentration, and when the treatment concentration is greater than 0.05mg / L, they are negatively correlated with the EBR concentration. The biomass under the culture condition of 0.05mg / L was the highest, reaching 1.54g / L, which was 1.29 times of the control group, 1.18 times of the culture of 0.1mg / L, 1.27 times of the culture of 0.15mg / L, and 1.27 times of the culture of 0.2mg / L 1.64 times lower. The specific growth rate also reached the maximum under the condition of 0.05 mg / L, which was 1.04 times that of the c...

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Abstract

The invention discloses a method for promoting accumulation of fucoxanthin and / or lipids in phaeodactylum tricornutum. The method is characterized by including the following steps: inoculating a seawater culture solution with a plurality of phaeodactylum tricornutum in a growing period according to the volume ratio of 10%, adding 2, 4-epibrassinolide into the seawater culture solution to enable the final concentration of the seawater culture solution to be 0.05-0.15mg / L, fully shaking a flask three times a day at the culture temperature of 20 DEG C under the conditions of illumination intensity being 5000lx and photoperiod being 12h / 12h, and culturing 10d of the phaeodactylum tricornutum, wherein the optimal concentration for fucoxanthin accumulation is 0.05mg / L, and the optimal concentration for lipid accumulation is 0.1mg / L. The method has the advantages that through addition of a certain amount of 2, 4-epibrassinolide in a culture medium, the accumulation speed of the fucoxanthin and / or the lipids in the phaeodactylum tricornutum can be increased effectively, and the content of the fucoxanthin and / or the lipids in the phaeodactylum tricornutum can be increased remarkably; the method is simple to operate and low in cost, and production cycle is short.

Description

technical field [0001] The invention relates to Phaeodactylum tricornutum, in particular to a method for promoting accumulation of fucoxanthin and / or lipid in Phaeodactylum tricornutum. Background technique [0002] Fucoxanthin is a class of carotenoids widely present in brown algae and diatoms. This pigment is not only found in macroalgae such as kelp and wakame, but also in microalgae, especially in diatoms. Its molecular structure contains unusual conjugated double bonds and 5,6-monoepoxy groups, which make it anti-cancer, anti-obesity, anti-inflammatory, anti-diabetic and anti-angiogenesis. [0003] The survival and development of human beings are inseparable from energy. Due to the limitation and non-renewability of traditional fossil fuels such as petroleum and coal, fossil fuels are increasingly depleted and cause serious environmental problems. It is imminent to find green renewable energy. Among many biofuels, microalgae have the advantages of short growth cycle, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12
Inventor 王小飞龚一富王何瑜
Owner NINGBO UNIV
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