Method of preparing ginseng saponine monomer from ginseng leaf

A technology of ginsenosides and ginseng leaves, which is used in pharmaceutical formulations, medical preparations containing active ingredients, steroids, etc.

Active Publication Date: 2006-11-29
HAINAN ASIA PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far people have isolated a variety of ginsenosides from ginseng leaves, mainly including ginsenoside F 1 , Ginsenoside Rg 1 , Ginsenoside Rg 2 , ginsenoside Re and other triol groups ginsenoside and ginsenoside Rb 1 , Ginsenoside Rb 2 , Ginsenoside Rb 3 , Ginsenoside Rc, Ginsenoside Rd, Ginse...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 7 kg of ginseng leaves, add water to decoct and extract three times, the amount of water added each time is 18, 14, and 12 times the weight of ginseng leaves, and the decoction time is 2, 1.5, and 1 hour respectively, and the decoction liquid is combined and passed through D4020 macropore adsorption Resin adsorption, rinse with water, elution with 85% ethanol until ginsenoside Re and ginsenoside Rd can not be detected in the eluent (silica gel thin layer chromatography, n-butanol: ethyl acetate: water=4: 1: 5 , upper layer, 10% sulfuric acid spray, 105 ℃ of heating color development, the same below), reclaim ethanol, obtain 980 grams of total saponins of ginseng leaves.

[0017] Take 12 grams of total saponins from ginseng leaves, put it on an alumina column, and elute with water until the eluent cannot detect ginsenoside F 1 , Ginsenoside Rg 1 and ginsenoside Re. The water eluate is absorbed by D4020 macroporous adsorption resin, eluted with 85% ethanol until no gins...

Embodiment 2

[0019] Take 12 grams of total saponins from ginseng leaves, put it on an alumina column, and elute with water until the eluent cannot detect ginsenoside F 1 , Ginsenoside Rg 1 and ginsenoside Re. The water eluate is absorbed by AB-8 macroporous adsorption resin, eluted with 85% ethanol until no ginsenoside F can be detected in the eluate 1 , Ginsenoside Rg 1 and ginsenoside Re, the solvent is recovered to obtain mainly ginsenoside F 1 , Ginsenoside Rg 2 , ginsenoside Re and ginsenoside Rg 1 The mixed saponins, namely mixed saponin A 6.3 grams. Then elute with 50% aqueous methanol solution, and the eluent reclaims the solvent to obtain mainly ginsenoside F 2 , the mixed saponin of Panax notoginsenoside Fe, ginsenoside Rd, i.e. mixed saponin B 1.8 grams. Finally, the aqueous solution of 50% tetrahydrofuran is used for elution, and the solvent is recovered from the eluent to obtain mainly ginsenoside Rd and ginsenoside Rb. 2 , Ginsenoside Rc, Ginsenoside Rb 1 and ginseno...

Embodiment 3

[0021] Take 12 grams of total saponins from ginseng leaves, put it on an alumina column, and elute with water until the eluent cannot detect ginsenoside F 1 , Ginsenoside Rg 1 and ginsenoside Re. The water eluate is absorbed by D101 macroporous adsorption resin, eluted with 85% ethanol until no ginsenoside F can be detected in the eluate 1 , Ginsenoside Rg 1 and ginsenoside Re, the solvent is recovered to obtain mainly ginsenoside F 1 , Ginsenoside Rg 2 , ginsenoside Re and ginsenoside Rg 1 The mixed saponins, namely mixed saponin A 7.4 grams. Then elute with 55% acetone aqueous solution, and the eluent reclaims the solvent to obtain mainly containing ginsenoside F 2 , mixed saponins of Panax notoginsenoside Fe, ginsenoside Rd, i.e. mixed saponin B 2.1 grams. Finally, the aqueous solution of 50% tetrahydrofuran is used for elution, and the solvent is recovered from the eluent to obtain mainly ginsenoside Rd and ginsenoside Rb. 2 , Ginsenoside Rc, Ginsenoside Rb 1 and ...

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PUM

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Abstract

A process for preparing ginsenoside monomer from ginseng leaf includes such steps as preparing general ginsenoside from ginseng leaf, chromatography with alumina column to obtain several groups of ginsenosides, and chromatography by column to obtain different ginsenoside monomers.

Description

technical field [0001] The invention relates to a method for extracting and separating various ginsenoside monomers from ginseng leaves, and more specifically discloses extracting and separating ginsenoside Rb from ginseng leaves 1 , Ginsenoside Rb 2 , Ginsenoside Rb 3 , Ginsenoside Rc, Ginsenoside Rd, Ginsenoside F 2 , Panax notoginsenoside Fe, ginsenoside Re, ginsenoside Rg 1 , Ginsenoside Rg 2 , Ginsenoside F 1 , especially extracting and separating ginsenoside F 1 , Ginsenoside F 2 1. The method of notoginseng saponin Fe belongs to the field of natural medicinal chemistry research. Background technique [0002] Ginseng leaves are the dried leaves of ginseng (Panax ginseng C.A.Mey.), harvested in autumn, dried or dried; it has the functions of invigorating qi, benefiting the lungs, dispelling heat, and promoting body fluid; it is used for cough due to qi deficiency, heat and irritability, and body fluid injury Thirst, blurred vision, tired limbs. [0003] So far ...

Claims

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Application Information

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IPC IPC(8): C07J9/00A61K31/704A61K36/258A61K127/00
Inventor 桂明玉金永日李绪文金永学
Owner HAINAN ASIA PHARM CO LTD
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