Method of preparing ginseng saponine monomer from ginseng leaf
A technology of ginsenosides and ginseng leaves, which is used in pharmaceutical formulations, medical preparations containing active ingredients, steroids, etc.
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Embodiment 1
[0016] 7 kg of ginseng leaves, add water to decoct and extract three times, the amount of water added each time is 18, 14, and 12 times the weight of ginseng leaves, and the decoction time is 2, 1.5, and 1 hour respectively, and the decoction liquid is combined and passed through D4020 macropore adsorption Resin adsorption, rinse with water, elution with 85% ethanol until ginsenoside Re and ginsenoside Rd can not be detected in the eluent (silica gel thin layer chromatography, n-butanol: ethyl acetate: water=4: 1: 5 , upper layer, 10% sulfuric acid spray, 105 ℃ of heating color development, the same below), reclaim ethanol, obtain 980 grams of total saponins of ginseng leaves.
[0017] Take 12 grams of total saponins from ginseng leaves, put it on an alumina column, and elute with water until the eluent cannot detect ginsenoside F 1 , Ginsenoside Rg 1 and ginsenoside Re. The water eluate is absorbed by D4020 macroporous adsorption resin, eluted with 85% ethanol until no gins...
Embodiment 2
[0019] Take 12 grams of total saponins from ginseng leaves, put it on an alumina column, and elute with water until the eluent cannot detect ginsenoside F 1 , Ginsenoside Rg 1 and ginsenoside Re. The water eluate is absorbed by AB-8 macroporous adsorption resin, eluted with 85% ethanol until no ginsenoside F can be detected in the eluate 1 , Ginsenoside Rg 1 and ginsenoside Re, the solvent is recovered to obtain mainly ginsenoside F 1 , Ginsenoside Rg 2 , ginsenoside Re and ginsenoside Rg 1 The mixed saponins, namely mixed saponin A 6.3 grams. Then elute with 50% aqueous methanol solution, and the eluent reclaims the solvent to obtain mainly ginsenoside F 2 , the mixed saponin of Panax notoginsenoside Fe, ginsenoside Rd, i.e. mixed saponin B 1.8 grams. Finally, the aqueous solution of 50% tetrahydrofuran is used for elution, and the solvent is recovered from the eluent to obtain mainly ginsenoside Rd and ginsenoside Rb. 2 , Ginsenoside Rc, Ginsenoside Rb 1 and ginseno...
Embodiment 3
[0021] Take 12 grams of total saponins from ginseng leaves, put it on an alumina column, and elute with water until the eluent cannot detect ginsenoside F 1 , Ginsenoside Rg 1 and ginsenoside Re. The water eluate is absorbed by D101 macroporous adsorption resin, eluted with 85% ethanol until no ginsenoside F can be detected in the eluate 1 , Ginsenoside Rg 1 and ginsenoside Re, the solvent is recovered to obtain mainly ginsenoside F 1 , Ginsenoside Rg 2 , ginsenoside Re and ginsenoside Rg 1 The mixed saponins, namely mixed saponin A 7.4 grams. Then elute with 55% acetone aqueous solution, and the eluent reclaims the solvent to obtain mainly containing ginsenoside F 2 , mixed saponins of Panax notoginsenoside Fe, ginsenoside Rd, i.e. mixed saponin B 2.1 grams. Finally, the aqueous solution of 50% tetrahydrofuran is used for elution, and the solvent is recovered from the eluent to obtain mainly ginsenoside Rd and ginsenoside Rb. 2 , Ginsenoside Rc, Ginsenoside Rb 1 and ...
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