α-l-arabinofuranosidase and its application in the preparation of ginsenoside rd

Abstract

The invention discloses alpha-L-arabinofuranosidase and application thereof in preparing ginsenoside Rd, and belongs to the field of gene engineering techniques and biological medicines. The amino acid sequence of alpha-L-arabinofuranosidase is as shown in SEQ ID NO.1. The alpha-L-arabinofuranosidase disclosed by the invention is high in conversion property for ginsenoside Rd, and after alpha-L-arabinofuranosidase and ginsenoside Rd are cultured for a certain time, the detection shows that ginsenoside Rc is nearly completely converted into ginsenoside Rd. Alpha-L-arabinofuranosidase is relatively high in resistance to arabinose and is not inhibited by glucose feedback.

Description

technical field

[0001] The invention belongs to the field of genetic engineering technology and biomedicine, and specifically relates to an α-L-arabinofuranosidase and its application, especially the application of enzymatic conversion of multi-component ginsenoside Rc to prepare ginsenoside Rd. Background technique

[0002] Ginseng (Panax ginseng C.A. Meyer) is a perennial herb that likes shade. The fleshy root of wild ginseng is a traditional precious Chinese herbal medicine in my country. It has the functions of regulating blood pressure, protecting the heart, resisting neurasthenia, and improving symptoms of diabetes. Modern medical research has proved that ginsenoside is one of the main effective components of ginseng. Ginsenosides have significant anti-cancer effects, making in-depth research on ginsenosides a hot topic.

[0003] According to the existing reports, more than 180 monomers of ginsenosides have been isolated and identified, but there are large differences ...

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