Method for determining content of ginsenoside in panax traditional Chinese medicine

A technology of ginsenosides and pseudo-ginsenosides, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult ginsenoside component detection, few index components, weak specificity, etc., and achieves perfect quality standards and strong specificity. , the effect of high sensitivity

Active Publication Date: 2021-09-24
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0003] However, due to the limitations of existing analytical techniques, it is difficult to conduct a comprehensive and accurate detection of ginsenoside components in traditional Chinese medicines of the genus Panax genus, and the current determination methods used for quality control of traditional Chinese medicines of the genus Panax genus lack universality and index components. Therefore, it is

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  • Method for determining content of ginsenoside in panax traditional Chinese medicine

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preparation example Construction

[0045] By adopting the preparation method of the sample solution to be tested in the present application, the sample solution to be tested including 15 kinds of ginsenosides can be obtained, so that the content detection results of the ginsenoside components in the sample to be tested are more comprehensive, accurate and credible.

[0046] In some embodiments of the present application, the chromatographic column is selected from any one of CORTECS UPLC Shield RP18, Kinetex EVO C18, BEH Shield RP18 or Atlantis Premier BEH C18 AX.

[0047] The inventors found in the research that using the gradient elution of the present application, the 15 kinds of ginsenosides can be separated better. Preferably, in some embodiments of the present application, the gradient elution is specifically: 0-7 minutes, 20% B; 7-9 minutes, 20-24% B; 9-32 minutes, 24-26% B; 32-72 minutes, 26-26% B; 72-80 minutes, 26-35 %B; 80-86 minutes, 35-50%B; 86-92 minutes, 50-60%B; 92-95 minutes, 60-98%B; 95-98 min...

Embodiment 1

[0065] Embodiment 1 chromatographic column is determined

[0066] Chromatographic conditions: select 12 chromatographic columns as shown in Table 3 for the chromatographic columns respectively.

[0067] Mobile phase: Phase A is 0.1% formic acid aqueous solution, phase B is acetonitrile; column temperature: 30°C; flow rate: 0.3mL / min; injection volume: 3μL; gradient elution: according to the different chromatographic columns in Table 3 for ginsenoside Two gradients were used to determine the different retention capacities.

[0068] Among them, when using the chromatographic columns with the numbers 1, 3, 5, and 7-9 in Table 3, the elution gradient: 0-7min, 20% B; 7-9min, 20-24% B; 9-24min, 24% -27%B; 24-50min, 27-27%B; 50-58min, 27-28%B; 58-62min, 28-32%B; 62-66min, 32-35%B; 66-71min, 35% -50%B; 71-75min, 50-65%B; 75-78min, 65-98%B; 78-81min, 98%B;

[0069]When using the chromatographic columns numbered 2, 4, 6, and 10-12 in Table 3, the elution gradient: 0-7min, 20% B; 7-11...

Embodiment 2

[0073] Embodiment 2 column temperature is determined

[0074] Chromatographic conditions: select the column temperature as 25°C, 28°C, 30°C, 35°C, and 40°C respectively.

[0075] Chromatographic column: CORTECS UPLC Shield RP18; mobile phase: phase A is 0.1% formic acid aqueous solution, phase B is acetonitrile; flow rate: 0.3mL / min; injection volume: 3μL; gradient elution: 0-7min, 20% B; 7 -9min, 20-24%B; 9-24min, 24-27%B; 24-50min, 27-27%B; 50-58min, 27-28%B; 58-62min, 28-32%B; 62 -66min, 32-35%B; 66-71min, 35-50%B; 71-75min, 50-65%B; 75-78min, 65-98%B; 78-81min, 98%B.

[0076] Detection conditions: atomization temperature: 40°C; data acquisition frequency: 5Hz; filter constant: 3.6s; power function: 1.00; gain: 100pA.

[0077] Preparation of the ginseng sample solution to be tested: take 1 g of the sample numbered PG-1 in Table 2, grind and pulverize, accurately weigh 100 mg into a 15 mL centrifuge tube, add 3 mL of 70% methanol aqueous solution for ultrasonic extraction ...

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Abstract

The invention provides a method for determining the content of ginsenoside in a panax traditional Chinese medicine, which adopts an ultra-high performance liquid chromatography electrospray detector method to simultaneously determine the content of 15 kinds of ginsenoside in the panax traditional Chinese medicine. The 15 kinds of ginsenoside comprise notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, 24 (R)-pseudoginsenoside F11, ginsenoside Rf, ginsenoside Ra2, ginsenoside Rb1, ginsenoside Rc, ginsenoside Ro, ginsenoside Rb2, ginsenoside Rb3, panax japonicus saponin IV, ginsenoside Rd, panax japonicus saponin IVa and 20 (R)-ginsenoside Rg3. By adopting the method disclosed by the invention, the contents of 15 kinds of ginsenoside in various panax traditional Chinese medicines can be simultaneously determined by reasonably selecting chromatographic conditions and detection conditions, and the method has the advantages of simplicity, convenience, accuracy, high sensitivity, strong specificity and the like, so that the method can be credibly, comprehensively and accurately used for quality control of the panax traditional Chinese medicines.

Description

technical field [0001] The application relates to the technical field of determination of components of traditional Chinese medicines, in particular to a method for determining the content of ginsenosides in traditional Chinese medicines of the genus Panax genus. Background technique [0002] Most of the traditional Chinese medicines belonging to ginseng are tonic Chinese medicines, among which ginseng, notoginseng, and American ginseng are valuable traditional Chinese medicines. The main active ingredient of ginseng traditional Chinese medicine is ginsenoside, which has the functions of preventing and treating cardiovascular diseases, enhancing immunity, calming the nerves, anti-cancer and anti-fatigue. [0003] However, due to the limitations of existing analytical techniques, it is difficult to conduct a comprehensive and accurate detection of ginsenoside components in traditional Chinese medicines of the genus Panax genus, and the current determination methods used for q...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/065
Inventor 杨文志李雪胡莹徐晓艳王思淼王洪达陈博学
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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