Preparation method of Alzheimer's disease animal model

A technology of Alzheimer's disease and animal models, applied to other methods of inserting foreign genetic materials, using microinjection methods, recombinant DNA technology, etc., can solve the problems of complicated pathogenesis and disease process, and achieve low lethality, Increase the probability of occurrence and the effect of good repeatability

Inactive Publication Date: 2011-09-21
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

At present, there are many ways to establish AD models, but due to the complexity of the pathogenesis and disease process of AD, there is no animal model that can fully reproduce and simulate all the characteristics of AD pathology, biochemistry, behavior, etc.

Method used

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  • Preparation method of Alzheimer's disease animal model
  • Preparation method of Alzheimer's disease animal model
  • Preparation method of Alzheimer's disease animal model

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Experimental program
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Embodiment 1

[0020] 1. Test material

[0021] 11. Experimental animals: clean-grade Kunming mice, female, 25-30 g, purchased from the Animal Experiment Center of Jiangsu University. The animals were randomly divided into a model group and a normal group, with 30 animals in the model group and 10 animals in the normal control group. The experimental animals were reared in separate cages, 5 animals per cage.

[0022] 12. Basic feed: Rat and mouse feed (provided by Jiangsu University)

[0023] 1.3. Reagents and instruments:

[0024] abcg4-siRNA: According to the mouse abcg4 gene sequence (NM-138955.3) registered on NCBI, primers were designed, and the abcg4 gene was cloned and sequenced. According to the sequencing results, siRNA was synthesized by Guangzhou Ruibo Biological Company (after testing, the synthetic Kunming mouse The specific target sequence of abcg4-siRNA is GTGAGAAGCAAGAGGTGAA); PBS buffer (NaCl 137mmol / L, KCl 2.7mmol / L, NaCl 2 HPO 4 4.3mmol / L, KH 2 PO 4 1.4mmol / L pH7....

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Abstract

The invention relates to a preparation method of an Alzheimer's disease animal model. The method comprises the following preparation steps: siRNA using animal brain abcg4 as target gene is designed; through the experiment, the target sequence of the specific siRNA which can interfere the expression of abcg4 is GTGAGAAGCAAGAGGTGAA, the siRNA is dissolved with PBS; and an healthy animal is selected, 240mu g / kg (weight) of siRNA is injected in the lateral ventricle of the animal once by using a stereotaxic apparatus, and the animal model is obtained after 48 hours. By adopting the method for preparing the animal model, the time is short and the damage to the experimental animal is not serious.

Description

technical field [0001] The invention relates to the technical field of establishing an animal model of Alzheimer's disease, in particular to a method for preparing an animal model for analyzing the pathology of Alzheimer's disease and screening Alzheimer's disease drugs. Background technique [0002] Alzheimer's disease (AD) is a common degenerative disease of the central nervous system, characterized by progressive memory and cognitive decline, abnormal behavior and social impairment. With the aging of the social population, the incidence of AD is on the rise, and it has become the fourth leading cause of death (after heart disease, tumor and stroke) that threatens the lives of the elderly, and it has become the most serious problem facing geriatrics. one of the problems. According to data, it is estimated that by 2050, the elderly aged 65 and over will account for about 35% of the total population in my country, and the elderly aged 80 and over will account for about 22%....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/89A01K67/027
Inventor 吴金美王雪芳栗凤鹏
Owner JIANGSU UNIV OF SCI & TECH
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