Modified yeast consuming L-arabinose

a technology of l-arabinose and modified yeast, which is applied in the direction of biofuels, microorganisms, biochemistry apparatus and processes, etc., can solve the problems of not being able to use these pentose sugars for growth, not being able to ferment xylose and l-arabinose, and having a relatively low ethanol toleran

Inactive Publication Date: 2005-06-30
FORSKARPATENT I SYD AB
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Problems solved by technology

But these superior glucose fermenting yeasts are unable to ferment xylose and L-arabinose and are also unable to use these pentose sugars for growth.
A few other yeast species such as Pichia sdpitis and Candida shehatae can ferment xylose to ethanol; however, they are not as effective as Saccharomyces for fermentation of glucose and have a relatively low ethanol tolerance.
Thus, they are not suitable for large scale industrial production of ethanol from biomass.
Some yeast can utilize L-arabinose for growth but no yeast can ferment it to commercial amounts of ethanol.
The strain hereby expresses araA, araB and araD, but is incapable of producing any ethanol.

Method used

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Embodiment Construction

[0007] It has now surprisingly been found possible to overcome the problem of having a yeast consuming L-arabinose by means of the present invention by obtaining a method for producing a L-arabinose utilizing yeast strain for the production of ethanol, which method is characterized in that a yeast strain is modified by introducing and expressing B. subtilis araA gene (L-arabinose isomerase), E. coil araB gene (L-ribulokinase) and E. coli araD gene (L-ribulose-5-P 4-epimerase), and carrying additional mutations in its genome or overexpressing the S. cerevisiae TAL1 (transaldolase) gene, enabling it to consume L-arabinose, and to produce ethanol.

[0008] The invention will be described more in detail in the following by reference to a number of experiments described explaining the nature of the invention.

[0009] The application further encompasses the Saccharomyces cerevisiae strain JBY25-4M (DSM 15560) and Saccharomyces cerevisiae strain JBY24-3T (DSM 15559) which were deposited at De...

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Abstract

The present invention relates to a method for producing a L-arabinose utilizing yeast strain for the production of ethanol, whereby a yeast strain is modified by introducing and expressing araA gene (L-arabinose isomerase), araB gene (L-ribulokinase D121-N) and araD gene (L-ribulose-5-P 4-epimerase) and carrying additional mutations in its genome or overexpressing a TAL1 (transaldolase) gene, enabling it to consume L-arabinose, to use it as the only carbon source, and to produce ethanol, as well as a method for producing ethanol using such a modified strain.

Description

TECHNICAL FIELD [0001] The present invention relates to a modified yeast strain, preferably a Saccharomyces cerevisiae, consuming L-arabinose while producing ethanol, as well as a method for producing ethanol. BACKGROUND OF THE INVENTION [0002] Fuel ethanol is considered as a suitable alternative to fossil fuels and it can be produced from plant biomass, which is a low cost and renewable resource available in large amounts. For this reason cellulose biomass, which includes agricultural residues, paper wastes, wood chips, etc., is an ideal abundantly available source of sugars for the fermentation to ethanol. For example when glucose is produced from cereals, hemi-cellulose-containing by-products mainly consisting of the pentose sugars arabinose and xylose (arabinoxylan) are generated. These are presently used as a low price cattle feed. But this resource could be utilized in a more profitable way if it would be integrated into existing starch processing which yields ethanol and star...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N1/18C12N1/19C12N15/52C12N15/74C12P7/06C12P19/02
CPCC12N15/52Y02E50/17C12P7/06Y02E50/10
Inventor BOLES, ECKHARDBECKER, JESSICA
Owner FORSKARPATENT I SYD AB
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