Synthesis method of sugar via extracellular enzyme catalysis

A synthesis method and technology of catalyzing sugar, applied in the direction of fermentation, etc., can solve the problems of complex structure reactor and low efficiency

Inactive Publication Date: 2012-03-21
黄卫东
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to overcome the dark reaction process of plant photosynthesis used in the existing artificial synthetic sugar, which is driven by input energy regeneration ATP, requires a complex structure reactor, and has low efficiency, and provides a new one that does not require input energy regeneration ATP , resulting in increased efficiency and a sugar synthesis method that does not require complex reactors

Method used

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  • Synthesis method of sugar via extracellular enzyme catalysis
  • Synthesis method of sugar via extracellular enzyme catalysis
  • Synthesis method of sugar via extracellular enzyme catalysis

Examples

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Effect test

Embodiment 1

[0045] Embodiment 1, formaldehyde is made raw material, produces glucose: in continuous flow reactor, water is made solvent, adds the required enzyme of reaction, and table 1 has listed these enzymes and the reaction that they catalyze, adds intermediate product 6 phosphate glucose in addition, Keeping the temperature of the reactor at 25°C, the reactant formaldehyde is continuously added to the reflux liquid and flows into the reactor, and the solution flows out continuously from the outlet of the reactor. After separating the glucose and removing part of the water, it flows back into the reactor. The concentration of formaldehyde in the solution at the inlet of the reactor is 30%, and the concentration of glucose at the outlet of the reactor is about 10%. Since no by-products can be detected, the formaldehyde is nearly 100% converted into glucose. In this embodiment, chemical catalysis can also be added, carbon dioxide is hydrogenated to formaldehyde, and the raw materials ar...

Embodiment 2

[0049] Embodiment 2, hydrogen and carbon dioxide are made raw materials, and product is starch: in continuous flow reactor, add water and do solvent, add the required enzyme of reaction, table 2 has listed these enzymes and the reaction that they catalyze, and enzyme is fixed at high On the molecular carrier, the intermediate product 6-phosphate glucose, NAD + , ATP, and maltotriose can also be added as a primer for synthesizing starch. Keep the temperature of the reactor at 25°C, continuously feed carbon dioxide and hydrogen into the reactor, and the solution will flow out continuously from the outlet of the reactor. After separating glucose and removing part of the water , backflow into the reactor. The starch concentration at the outlet of the reactor was about 8%, and hydrogen and carbon dioxide were nearly 100% converted into starch because no by-products were detected.

[0050] Table 2: Reactions and enzymes used in artificial photosynthesis dark reaction pathway driven...

Embodiment 3

[0053] Example 3, hydrogen and coal-fired power plant exhaust gas are used as raw materials, and the product is starch: a carbon dioxide fixation reactor, a sugar synthesis reactor and a separator are used to form a system, and water is used as a solvent. When starting the reactor, add synthetic starch to the reactor aqueous solution. All the enzymes required for the sugar reaction, Table 3 shows these enzymes and the reactions they catalyze, and the intermediate product 6-phosphate glucose, NAD + , ATP, keep the reactor temperature at 25°C, feed gas containing carbon dioxide into the carbon dioxide fixation reactor, such as waste gas containing 10% carbon dioxide from a coal-fired power plant, feed high-purity hydrogen into the sugar synthesis reactor, and react The liquid stays in the carbon dioxide fixation reactor for a period of time, carries out the carbon dioxide fixation reaction under the enzyme catalysis, and then discharges into the sugar synthesis reactor, stays for...

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Abstract

The invention discloses a synthesis method of sugar via extracellular enzyme catalysis, comprising steps that: formaldehyde is reacted with 5-ribulose phosphate under the enzyme catalysis to produce 6-fructose phosphate; the 6-fructose phosphate is converted to the sugar under the enzyme catalysis, or connects glycosyl to an amylum chain or a cellulose chain. The formaldehyde can be produced by hydrogenating or electrolyzing carbon dioxide via the enzyme catalysis or chemical catalysis. The synthesis method also can comprise the step of using the 6-fructose phosphate to regenerate the 5-ribulose phosphate, so as to continuously produce the sugar. The synthesis method of the sugar via the extracellular enzyme catalysis uses a simple reactor, and can adopt the energy-fixed carbon dioxide to industrially produce the sugar.

Description

Technical field [0001] The invention belongs to the synthesis of organic matter, in particular to the synthesis of sugar. Background technique [0002] Sugar is the main component of human food, which is mainly derived from plant photosynthesis. Plant photosynthesis includes light reactions that use chlorophyll to absorb solar energy and transfer energy to NADPH and ATP, and dark reactions that use NADPH and ATP to convert carbon dioxide and water to form carbohydrates. The photosynthesis efficiency of plants in nature is low. Theoretically, the maximum photosynthesis efficiency of plants is only 4.6-6.0%. The main reasons for the low efficiency include, first, chlorophyll can only absorb 400-700 Nanophotons; second, every time 8 photons are absorbed, only 2 NADPH molecules and 3 ATP molecules needed to transform a molecule of carbon dioxide can be produced. Even if calculated according to the energy of red light photons, only 43% of the absorbed energy is transferred to O...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02
Inventor 黄卫东
Owner 黄卫东
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