45 results about "Fructose phosphate" patented technology

The invention relates to a recombinant yeast, a construction method thereof and application thereof for producing tyrosol and a derivative. The recombinant yeast is acquired by introducing an exogenous fructose-6-phosphate phosphoketolase into an engineered yeast cell, and the engineered yeast cell is a yeast cell having a metabolic pathway for the synthesis of tyrosol via erythrose-4-phosphate and phosphoenolpyruvate. It is disclosed for the first time that in the process of expressing the fructose-6-phosphate phosphoketolase in the yeast, fructose-6-phosphate is catalyzed into erythrose-4- phosphate and acetyl phosphate while synthesizing 1,6-fructose diphosphate, xylulose-5-phosphate is catalyzed into glyceraldehydes-3-phosphate and acetyl phosphate, which alters the distribution of carbon metabolism in yeast, and enhances the synthesis of erythrose-4-phosphate, an important intermediate of tyrosol biosynthesis. The metabolic pathway of synthetic tyrosol is optimized, and the yieldof derivatives such as tyrosol and hydroxytyrosol is improved.

The invention provides a high-energy nutrient composition. Active ingredients of the high-energy nutrient composition include, by weight, 500-1000 parts of 1, 6-fructose bisphosphate, 200-320 parts of manyprickle acanthopanax root, 10-16 parts of rhizoma polygonati extract, and 1.2-1.6 part of wolfberry extract. The high-energy nutrient composition can not only provide high energy, but also can enhance oxidation resistance of a human body and is suitable for a user to take during a large amount of exercise, so that aerobic endurance is improved and fatigue recovery is promoted.

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.

The application relates to the field of agricultural biotechnology, in particular to an Escherichia coli engineering bacterium and a fermentation production method of N-acetylglucosamine. The present invention improves the supply of product precursors in mutant Escherichia coli chassis cells by cutting off the shunting of N-acetylglucosamine synthesis precursor 6-fructose phosphate by truncating the glycolysis pathway and the pentose phosphate pathway, and then utilizing the free high-copy The plasmid expresses the key enzymes of the N-acetylglucosamine synthesis pathway in the chassis cells, and the mixed carbon source medium is used to ferment and produce N-acetylglucosamine, and the yield of N-acetylglucosamine reaches 2.8 g / L.

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.

The present invention relates to a composition for preventing white hair, promoting black hair, and preventing, improving or treating vitiligo or hypopigmentation, comprising fructose 1, 6-diphosphate or a salt, a solvate, a stereoisomer or a hydrate thereof as an active ingredient, the invention aims to solve the problem that in the prior art, the influence on the formation of melanin is not large, and the feeling of cutting is not given out. According to the present invention, the composition comprises fructose 1, 6-diphosphate or a salt, solvate, stereoisomer or hydrate thereof, and thus has excellent efficacy in preventing white hair, promoting black hair, and treating vitiligo or hypopigmentation.