42 results about "Fructose phosphate" patented technology

The invention discloses cell frozen stock solution for freezing and storing nerve cells and a freezing storage method, wherein, the cell frozen stock solution and the freezing storage method are used for freezing and storing the nerve cells, especially primary animal nerve cells that are isolated freshly, such as hippocampus nerve cells. The frozen stock solution is formed by adding basic medium,fetal bovine serum, dimethyl sulfoxide,6-fructose phosphate and flavonoid compounds to the basic medium; 6-fructose phosphate (w/w) achieves the function of improving cell viability (effective cell rate) when the frozen cells are recovered; and in addition, the flavonoid compounds achieve definite oxidation resistance and can prevent free radical from damaging the cells. The cell frozen stock solution and the freezing storage method can improve the survival rate and the cell function of the nerve cells through freezing storage, the survival rate of unfreezing cells can be increased to reach more than 95 percent, the frozen stock solution for the nerve cells can preserve the nerve cells for a long period, the biological activity of the nerve cells can be ensured, and very high utility values can be achieved.

The invention discloses culture solution for primary culture of newly born rat hippocampal neuron and a method for primarily culturing the rat hippocampal neuron. The culture solution is formed by adding 1,6 diphosphonic acid fructose, fructose and nutrition additive into base culture solution Neurobasalmedium. The method for primarily culturing the rat hippocampal neuron comprises the following steps: 1 preparing hippocampal neuron culture solution; 2 drawing materials and digesting the materials; 3 preparing single cell suspension; and 4 conducting inoculating and cultivating. The method for primarily culturing the rat hippocampal neuron is simple, convenient and capable of obtaining hippocampal neuron with good growth state and high purity by adopting the culture solution and has important application value and economical value.

The invention provides the application of a 1,6-fructose diphosphate sodium salt, fructose and / or borneol composition to the preparation of an antiepileptic drug. The composition consists of 1,6-fructose diphosphate sodium salt and fructose, or 1,6-fructose diphosphate sodium salt and borneol combination, or 1,6-fructose diphosphate sodium salt, fructose and borneol. The composition provided by the invention can improve in vivo stability of 1, 6 1,6-fructose diphosphate, in order to maintain high level of 1,6-fructose diphosphate in the brain for a long time and make the 1,6-fructose diphosphate sodium salt exert antiepileptic effect for a long time. The composition provided by the invention particularly employs chewable tablets, orally disintegrating tablets and dispersible tablets as dosage forms for the antiepileptic drug, and the dosage forms can be directly absorbed in oral mucosa, avoid hepatic first-pass effect and further increase the level of 1,6-fructose diphosphate in the brain, thereby further improving the antiepileptic efficacy of the drug.

The invention belongs to the technical field of bioengineering, and relates to construction of genetic engineering strains, in particular to recombinant escherichia coli and a construction method andapplication thereof. According to the recombinant escherichia coli, an undecylenyl phosphate glucose phosphotransferase gene wcaJ is knocked out, manB, manC, gmd, wcaG and srlD genes are recombined and expressed, a pntAB gene, an ndK gene and a gsK gene are further recombined and expressed, and a pfkA gene is knocked out, so that a synthesis path of forming fructose 6-phosphate is enhanced and theglycolysis process of 6-fructose phosphate is weakened, the accumulation of fructose 6-phosphate is facilitated, and thus more precursor substances are provided for the synthesis of GDP-L-fucose; inaddition, the regeneration of the cofactors NADPH and GTP is enhanced, so that the capacity of producing GDP-L-fucose by the recombinant escherichia coli strain is further improved.

The invention discloses a sports nutritional composition and a preparation method thereof, which relates to the technical field of sports nutrition foods or sports beverages. The sports nutrition composition comprises following raw materials: 1, 6-diphosphate fructose trisodium salt, nicotinamide mononucleotide, guarana sodium extract, couch grass root extract, rhizoma phragmitis extract, soybeanpeptide, wheat oligopeptide, compound amino acid, compound vitamin, carbohydrate, medium-chain triglyceride microcapsule powder, creatine, taurine and compound minerals. The sports nutrition composition provided by the invention not only contains basic nutrients required by sports people, but also improves the athletic ability, prevents or delays the generation of fatigue and improves the exerciseendurance by regulating energy metabolism, perfecting the body's antioxidant system in a sports state, improving the muscle state and the like.

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.

The invention discloses a composition. The composition contains a silybum marianum extract and fructose 1, 6 diphosphate or a derivative thereof, or a salt thereof. The silybum marianum extract is ina silybum marianum extract microcapsule form and/or a silybum marianum extract liposome form and/or a silybum marianum extract self-microemulsifying form, and the composition of the silybum marianum extract and FDP has the effects of reducing liver injury, preventing hangover and resisting fatigue, and particularly can quickly take effect to reduce liver injury and quickly sober up.

The invention provides a high-energy nutrient composition. Active ingredients of the high-energy nutrient composition include, by weight, 500-1000 parts of 1, 6-fructose bisphosphate, 200-320 parts of manyprickle acanthopanax root, 10-16 parts of rhizoma polygonati extract, and 1.2-1.6 part of wolfberry extract. The high-energy nutrient composition can not only provide high energy, but also can enhance oxidation resistance of a human body and is suitable for a user to take during a large amount of exercise, so that aerobic endurance is improved and fatigue recovery is promoted.

The invention discloses a gene sequence of Oryza sativa Japonica Group glutamine-fructose-6-phosphate aminotransferase (OsGFAT) derived from Oryza sativa Japonica Group, and application of the gene sequence. The invention provides a method for preparing the OsGFAT. The method comprises the following steps: cloning the gene of the OsGFAT to an escherichia coli expression vector to obtain an escherichia coli recombinant strain capable of heterologously expressing the OsGFAT, and preparing OsGFAT by heterologously expressing the strain. The invention also provides the application of the OsGFAT. The OsGFAT is used for catalytic synthesis of GlcN-6-P and Glc-6-P by an enzymic method.

The invention provides a formula and a preparation method of bone peptide composite food, and relates to the technical field of sports food. The formula of the bone peptide composite food comprises the following raw materials in parts by mass: 75 to 100 parts of polydextrose, 68 to 88 parts of bovine collagen peptide powder, 30 to 50 parts of creatine, 30 to 40 parts of milk mineral salt, 0.08 to 0.4 part of tricalcium phosphate, 3 to 8 parts of lindera aggregata leaf powder, 0.1 to 0.8 part of fructo-oligosaccharide, 0.09 to 0.45 part of saussurea involucrata culture powder, 5 to 12 parts of casein phosphopeptide powder, 0.09 to 0.45 part of chitosan oligosaccharide, 0.06 to 0.2 part of magnesium oxide, 0.09 to 0.1 part of 1, 6-fructose diphosphate trisodium salt, 1 to 4 parts of ginseng powder, 2 to 7 parts of rhizoma polygonati powder, 3 to 10 parts of fructus lycii powder, 1 to 8 parts of rhizoma curcumae longae powder, 2 to 6 parts of fructus hippophae powder, 3 to 8 parts of bovine bone peptide powder, 0.08 to 0.12 part of sodium hyaluronate, 0.02 to 0.08 part of vitamin C, 0.04 to 0.06 part of ferric pyrophosphate, 0.02 to 0.05 part of vitamin B6, 0.02 to 0.05 part of vitamin B12, 0.03 to 0.06 part of vitamin D, 0.09 to 0.5 part of citric acid and 0.09 to 0.45 part of stevioside. The bone peptide composite food is high in nutrient and good in eating effect through matching of various substances.

The invention discloses application of fructose-1, 6-diphosphate in improving the anti-hypoxia ability of the body. The invention discloses application of fructose-1, 6-diphosphate in preparing products for improving the anti-low oxygen or anti-hypoxia ability of animals. Fructose-1, 6-diphosphate serves as a human body metabolic intermediate, exogenous supplementation of fructose-1, 6-diphosphate can improve the body's resistance to plateau hypoxia, and improve the athletic ability and anti-fatigue ability of the body in a plateau hypoxia environment.

The invention relates to a heart-nourishing food health-preserving tea granule and belongs to the field of nutritious foods. Purposes of the tea granule are good effect and high effectiveness. The tea granule is prepared by raw materials such as poria cocos, peach kernel, gynostemma pentaphylla, 1.6-fructose diphosphate and folium mori, has effects of strengthening body resistance, activating blood, dissolving stasis, nourishing a heart and invigorating a pulse, and has the advantages of good effect and high effectiveness.

The invention provides a sports nutritional food formula and a preparation method thereof. Every 30 g of the sports nutritional food comprises the following raw materials of, by weight, 9.5-10.80 g of anhydrous dextrose, 6.4-8.2 g of maltooligosaccharide, 6.9-9.2 g of cane sugar, 3.5-5.5 g of crystalline fructose, 0.2-0.3 g of 1.6-fructose diphosphate trisodium salt, 0.75-0.9 g of L-carnitine, 0.05-0.15 g of edible salt sodium chloride, 1-3 g of grape seed extract, 0.4-0.8 g of tea polyphenol, 0.4-0.6 mg of thiamine hydrochloride, 0.4-0.6 mg of riboflavin, 0.4-0.6 mg of pyridoxine hydrochloride, 0.1-0.2 g of sodium bicarbonate and 0.5-0.7 g of monopotassium phosphate. According to the formula, carbohydrate distribution is reasonable, sodium salt content proportion is scientific, concentration coordination of carbohydrate and sodium salt is achieved, vitamin B content proportion is reasonable, the effect of exciting the cerebral cortex is achieved, and the purpose of refreshing can be achieved; and in addition, the formula also has the effect of shrinking blood vessels, is beneficial to prolonging the exercise time, is also beneficial to reducing the body fat content, and is beneficial to relieving the exercise oxidative stress.

The invention relates to the technical field of functional foods, in particular to a nutritional supplement. The nutritional supplement comprises the following raw materials in parts by weight of 720-900 parts of carbohydrate, 20-80 parts of 1, 6-fructose diphosphate, 15-70 parts of taurine, 12-70 parts of plant polyphenol and 0.1-0.8 part of vitamin B. The ORAC value of the plant polyphenol is not less than 15000 mu mol TE/g, and the content of oligomeric proanthocyanidins is not less than 45%. The nutritional supplement disclosed by the invention can be stably compounded, and is good in water solubility, proper in osmotic pressure and quick to absorb; and the athletic ability of athletes can be improved, and important organs and tissues of bodies can be protected.

The invention discloses a preparation method of a healthy feed additive for protecting hepatopancreas of procambarus clarkii, and belongs to the technical field of healthy breeding of shrimps and crabs. The preparation method comprises the following specific preparation processes: weighing microcrystalline cellulose, glucose, glucose-6-phosphoric acid, fructose-6-phosphoric acid, trehalose, glutamine, glutamic acid, glutamide-6-phosphoric acid, glucosamine, taurine, cholesterol and a mixture of trehalose and glucosamine according to mass parts, uniformly mixing, and storing the mixed mixture under a light-closed refrigeration condition. According to the invention, after the prepared healthy feed additive for protecting hepatopancreas of procambarus clarkii is uniformly mixed with commercialized procambarus clarkia feed according to a ratio of 1: 49, the obtained mixture is easy to absorb, the hepatopancreas form and function of the procambarus clarkia can be remarkably protected, the survival rate and weight gain rate of the procambarus clarkia are increased, and the culture benefit is obviously improved.

The invention provides a treatment agent for laying hen pecking and a preparation method thereof. Firstly, serratia marcescens ATCC14756 and clostridium butyricum ATCC19398 are respectively activatedand singly fermented, and are then mixed to prepare seed liquid; next, rhizoma acori tatarinowii and spirulina are mixed and extracted to prepare mixed slurry; finally, the mixed slurry is added intothe seed liquid; and mixed fermentation is performed. The serratia marcescens and the clostridium butyricum can produce various metabolites after the fermentation treatment; and antibacterial performance and immune enhancing performance are realized. The rhizoma acori tatarinowii has the sedation effect. The spirulina can be used for regulating various body functions. D-fructose-1,6-diphoshatecalcium salt can provide an energy source on one hand, and can provide a calcium source on the other hand. By using the treatment agent provided by the invention, antibacterial and anti-inflammatory effects can be achieved; the occurrence and diffusion of other complication diseases can be avoided; the sedation effect is achieved on laying hens; the further diffusion of the pecking is primarily controlled; meanwhile, the immunity of the laying hens is improved; the requirements of the laying lens on amino acids, vitamins and mineral substances are met; and the reoccurrence of the laying hen pecking is avoided from the source.

The invention discloses an application of 1,6-fructose diphosphate and salt formed by the1,6-fructose diphosphate in the preparation of medicine for preventing and treating human enveloped virus infection and virus inactivating agents. The copying level of a variety of common human viruses pretreated by 1,6-fructose diphosphate sodium salt on cells is detected, and the virus killing activity of the 1,6-fructose diphosphate sodium salt on the various human viruses is evaluated. Results show that the 1,6-fructose diphosphate sodium salt has dose-dependent virus killing activity on enveloped viruses. Experiments prove that the virus killing function of the 1,6-fructose diphosphate sodium salt depends on temperature. The 1,6-fructose diphosphate is a glycolysis mesostate, and meanwhile the function of adjusting metabolism is achieved. The compound can inactivate a variety of enveloped viruses, and a broad prospect of developing the compound into the medicine for preventing and treating human enveloped virus infection and the virus inactivating agents is achieved.

The invention discloses a pharmaceutical composition and application thereof, and the pharmaceutical composition is composed of 1, 6-fructose diphosphate (FDP) or a pharmaceutically acceptable salt thereof, and nicotinamide mononucleotide NMN/nicotinamide adenine dinucleotide NAD + or a pharmaceutically acceptable salt thereof. The invention also discloses application of the compound in preparation of medicines for preventing and/or treating primary dysmenorrhea and infertility. The invention discloses an action mechanism of FDP and NMN/NAD < + > combined administration in treating a mouse primary dysmenorrhea model, and finds that the FDP and NMN/NAD < + > combined administration has a better curative effect than FDP or NMN/NAD < + > independent administration, and can obviously reduce the writhing reaction frequency of a female mouse, increase the incubation period of the writhing reaction, reduce the gene expression level of COX-2 and PGF2alpha in uterine tissues, increase the gene expression level of PGE2, and improve the mouse primary dysmenorrhea treatment effect. Infiltration of inflammatory cells is reduced, and uterine tissue edema and inflammatory pathology degree are improved; in addition, combined administration of FDP and NMN/NAD + can significantly increase the pregnancy rate of female mice compared with independent administration of FDP or NMN/NAD +.