65 results about "Fructose phosphate" patented technology

The invention discloses cell frozen stock solution for freezing and storing nerve cells and a freezing storage method, wherein, the cell frozen stock solution and the freezing storage method are used for freezing and storing the nerve cells, especially primary animal nerve cells that are isolated freshly, such as hippocampus nerve cells. The frozen stock solution is formed by adding basic medium,fetal bovine serum, dimethyl sulfoxide,6-fructose phosphate and flavonoid compounds to the basic medium; 6-fructose phosphate (w / w) achieves the function of improving cell viability (effective cell rate) when the frozen cells are recovered; and in addition, the flavonoid compounds achieve definite oxidation resistance and can prevent free radical from damaging the cells. The cell frozen stock solution and the freezing storage method can improve the survival rate and the cell function of the nerve cells through freezing storage, the survival rate of unfreezing cells can be increased to reach more than 95 percent, the frozen stock solution for the nerve cells can preserve the nerve cells for a long period, the biological activity of the nerve cells can be ensured, and very high utility values can be achieved.

The invention discloses culture solution for primary culture of newly born rat hippocampal neuron and a method for primarily culturing the rat hippocampal neuron. The culture solution is formed by adding 1,6 diphosphonic acid fructose, fructose and nutrition additive into base culture solution Neurobasalmedium. The method for primarily culturing the rat hippocampal neuron comprises the following steps: 1 preparing hippocampal neuron culture solution; 2 drawing materials and digesting the materials; 3 preparing single cell suspension; and 4 conducting inoculating and cultivating. The method for primarily culturing the rat hippocampal neuron is simple, convenient and capable of obtaining hippocampal neuron with good growth state and high purity by adopting the culture solution and has important application value and economical value.

The invention discloses a synthesis method of sugar via extracellular enzyme catalysis, comprising steps that: formaldehyde is reacted with 5-ribulose phosphate under the enzyme catalysis to produce 6-fructose phosphate; the 6-fructose phosphate is converted to the sugar under the enzyme catalysis, or connects glycosyl to an amylum chain or a cellulose chain. The formaldehyde can be produced by hydrogenating or electrolyzing carbon dioxide via the enzyme catalysis or chemical catalysis. The synthesis method also can comprise the step of using the 6-fructose phosphate to regenerate the 5-ribulose phosphate, so as to continuously produce the sugar. The synthesis method of the sugar via the extracellular enzyme catalysis uses a simple reactor, and can adopt the energy-fixed carbon dioxide to industrially produce the sugar.

The invention provides the application of a 1,6-fructose diphosphate sodium salt, fructose and / or borneol composition to the preparation of an antiepileptic drug. The composition consists of 1,6-fructose diphosphate sodium salt and fructose, or 1,6-fructose diphosphate sodium salt and borneol combination, or 1,6-fructose diphosphate sodium salt, fructose and borneol. The composition provided by the invention can improve in vivo stability of 1, 6 1,6-fructose diphosphate, in order to maintain high level of 1,6-fructose diphosphate in the brain for a long time and make the 1,6-fructose diphosphate sodium salt exert antiepileptic effect for a long time. The composition provided by the invention particularly employs chewable tablets, orally disintegrating tablets and dispersible tablets as dosage forms for the antiepileptic drug, and the dosage forms can be directly absorbed in oral mucosa, avoid hepatic first-pass effect and further increase the level of 1,6-fructose diphosphate in the brain, thereby further improving the antiepileptic efficacy of the drug.

The invention belongs to the technical field of bioengineering, and relates to construction of genetic engineering strains, in particular to recombinant escherichia coli and a construction method andapplication thereof. According to the recombinant escherichia coli, an undecylenyl phosphate glucose phosphotransferase gene wcaJ is knocked out, manB, manC, gmd, wcaG and srlD genes are recombined and expressed, a pntAB gene, an ndK gene and a gsK gene are further recombined and expressed, and a pfkA gene is knocked out, so that a synthesis path of forming fructose 6-phosphate is enhanced and theglycolysis process of 6-fructose phosphate is weakened, the accumulation of fructose 6-phosphate is facilitated, and thus more precursor substances are provided for the synthesis of GDP-L-fucose; inaddition, the regeneration of the cofactors NADPH and GTP is enhanced, so that the capacity of producing GDP-L-fucose by the recombinant escherichia coli strain is further improved.

The invention discloses a sports nutritional composition and a preparation method thereof, which relates to the technical field of sports nutrition foods or sports beverages. The sports nutrition composition comprises following raw materials: 1, 6-diphosphate fructose trisodium salt, nicotinamide mononucleotide, guarana sodium extract, couch grass root extract, rhizoma phragmitis extract, soybeanpeptide, wheat oligopeptide, compound amino acid, compound vitamin, carbohydrate, medium-chain triglyceride microcapsule powder, creatine, taurine and compound minerals. The sports nutrition composition provided by the invention not only contains basic nutrients required by sports people, but also improves the athletic ability, prevents or delays the generation of fatigue and improves the exerciseendurance by regulating energy metabolism, perfecting the body's antioxidant system in a sports state, improving the muscle state and the like.

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.

The invention discloses a composition. The composition contains a silybum marianum extract and fructose 1, 6 diphosphate or a derivative thereof, or a salt thereof. The silybum marianum extract is ina silybum marianum extract microcapsule form and / or a silybum marianum extract liposome form and / or a silybum marianum extract self-microemulsifying form, and the composition of the silybum marianum extract and FDP has the effects of reducing liver injury, preventing hangover and resisting fatigue, and particularly can quickly take effect to reduce liver injury and quickly sober up.

The invention relates to a sport nutritional probiotic composition and a preparation method thereof. The sport nutritional probiotic composition comprises the following raw materials in parts by weight: 10 to 30 parts of creatine, 1 to 3 parts of 1,6-fructose diphosphate, 0.5 to 1 part of lactobacillus plantarum TWK10, 10 to 20 parts of isomalto-oligosaccharide, 5 to 15 parts of fructo-oligosaccharide, 10 to 20 parts of xylooligosaccharide, 0.15 to 0.25 part of lactobacillus rhamnosus Lr-G14, 0.17 to 0.26 part of lactobacillus acidophilus LA-G80, 0.2 to 0.3 part of bifidobacterium bifidum BB-G90 and 0.3 to 0.4 part of bifidobacterium lactis BL-G101. According to the composition, the creatine, the fructose 1,6-diphosphate, the lactobacillus plantarum TWK10, prebiotics and probiotics are taken as raw materials to form the sport nutritional probiotic composition, so that the sport performance can be effectively improved, the fatigue can be relieved, the muscle strength can be increased, the intestinal florae can be adjusted, and the gastrointestinal sport stress symptom can be relieved.

The invention provides a construction method of an escherichia coli synthetic strain capable of efficiently fermenting isobutanol in a low-residual-sugar manner. Escherichia coli MG 1655 is taken as a host, an isobutanol synthesis strain E.coli LA09 is constructed, and the fact that a conversion reaction from intracellular 6-phosphofructose into 1,6-fructose bisphosphate is a key limitation factor of residual sugar conversion is determined with a metabonomics method. Heterogenous artificial ED metabolic pathway upstream and downstream fragments derived from a strain Zymomonas mobilis ZM4 are constructed, ED upstream and downstream pathways are matched and adjusted with an artificial promoter in combination of synthetic biology, and one escherichia coli synthetic strain E.coli ED capable of efficiently fermenting isobutanol in the low-residual-sugar manner is constructed; with the initial glucose concentration being 45 g / L, fermentation is completed within 30 h, the yield of isobutanol reaches 13.67 g / L, and the fermenting residual sugar concentration is 0.87 g / L, thereby proving that the method is practical.

The invention provides a high-energy nutrient composition. Active ingredients of the high-energy nutrient composition include, by weight, 500-1000 parts of 1, 6-fructose bisphosphate, 200-320 parts of manyprickle acanthopanax root, 10-16 parts of rhizoma polygonati extract, and 1.2-1.6 part of wolfberry extract. The high-energy nutrient composition can not only provide high energy, but also can enhance oxidation resistance of a human body and is suitable for a user to take during a large amount of exercise, so that aerobic endurance is improved and fatigue recovery is promoted.

The invention relates to a recombinant yeast, a construction method thereof and an application of the recombinant yeast in preparing tyrosol and derivatives. The recombinant yeast is obtained by introducing an exogenous fructose-6-phosphate phosphoketolase expression gene into a modified yeast cell, and the modified yeast cell is a yeast cell with a metabolic pathway of synthesizing tyrosol through 4-erythrose phosphate and phosphoenolpyruvic acid. The invention first discloses the fact that in a process of expressing fructose-6-phosphate phosphoketolase in yeast, fructose-6-phosphoric acid iscatalyzed into erythrose-4-phosphoric acid and acetyl phosphoric acid while 1,6-fructose diphosphate is synthesized, and xylulose-5-phosphoric acid is catalyzed into glyceraldehyde-3-phosphoric acidand acetyl phosphoric acid, so that distribution of the carbon metabolism flow in yeast is changed, synthesis of an important intermediate substance erythrose-4-phosphoric acid in tyrosol biosynthesisis enhanced, the metabolic pathway for synthesizing tyrosol is optimized, and the yield of tyrosol and derivatives such as hydroxytyrosol and the like is improved.

The invention discloses a gene sequence of Oryza sativa Japonica Group glutamine-fructose-6-phosphate aminotransferase (OsGFAT) derived from Oryza sativa Japonica Group, and application of the gene sequence. The invention provides a method for preparing the OsGFAT. The method comprises the following steps: cloning the gene of the OsGFAT to an escherichia coli expression vector to obtain an escherichia coli recombinant strain capable of heterologously expressing the OsGFAT, and preparing OsGFAT by heterologously expressing the strain. The invention also provides the application of the OsGFAT. The OsGFAT is used for catalytic synthesis of GlcN-6-P and Glc-6-P by an enzymic method.

The invention provides application of 1, 6-fructose bisphosphate sodium salt containing borneol to preparation of antiepileptic drug, wherein the 1,6-fructose bisphosphate sodium salt and borneol are in the proportion of 100:0.01-100:20. Research shows that borneol can enhance the antiepileptic effect of 1, 6-fructose bisphosphate sodium salt; the synergistic effect of borneol is related to increase of fructose bisphosphate sodium salt level in the brain; and combination borneol can significantly improve the antiepileptic therapeutic effect. The medicament is an oral solid preparation selected from a chewing tablet, a dispersible tablet, an orally disintegrating tablet or a common tablet, and is an efficient antiepileptic candidate medicament.

A recombinant yeast is constructed by introducing an expressed gene of exogenous Fructose-6-phosphate phosphoketolase into a modified yeast cell, and the modified yeast cell is a yeast cell with a metabolic pathway for synthesizing tyrosol via Erythrose-4-phosphate and phosphoenolpyruvate. The present invention discloses for the first time that in the process of expressing Fructose-6-phosphate phosphoketolase in a yeast, Fructose-6-phosphate is synthesized into beta-D-Fructose 1,6-bisphosphate and also catalyzed into Erythrose-4-phosphate and Acetyl-phosphate, and Xylulose-5-phosphate is catalyzed into Glyceraldehydes-3-phosphate and Acetyl-phosphate, which change the metabolic flux distribution of carbon in the yeast, enhance the synthesis of Erythrose-4-phosphate as an important intermediate for the biosynthesis of tyrosol, optimize the metabolic pathway for synthesizing tyrosol, and increase the yields of tyrosol and its derivatives such as hydroxytyrosol.

The invention provides a formula and a preparation method of bone peptide composite food, and relates to the technical field of sports food. The formula of the bone peptide composite food comprises the following raw materials in parts by mass: 75 to 100 parts of polydextrose, 68 to 88 parts of bovine collagen peptide powder, 30 to 50 parts of creatine, 30 to 40 parts of milk mineral salt, 0.08 to 0.4 part of tricalcium phosphate, 3 to 8 parts of lindera aggregata leaf powder, 0.1 to 0.8 part of fructo-oligosaccharide, 0.09 to 0.45 part of saussurea involucrata culture powder, 5 to 12 parts of casein phosphopeptide powder, 0.09 to 0.45 part of chitosan oligosaccharide, 0.06 to 0.2 part of magnesium oxide, 0.09 to 0.1 part of 1, 6-fructose diphosphate trisodium salt, 1 to 4 parts of ginseng powder, 2 to 7 parts of rhizoma polygonati powder, 3 to 10 parts of fructus lycii powder, 1 to 8 parts of rhizoma curcumae longae powder, 2 to 6 parts of fructus hippophae powder, 3 to 8 parts of bovine bone peptide powder, 0.08 to 0.12 part of sodium hyaluronate, 0.02 to 0.08 part of vitamin C, 0.04 to 0.06 part of ferric pyrophosphate, 0.02 to 0.05 part of vitamin B6, 0.02 to 0.05 part of vitamin B12, 0.03 to 0.06 part of vitamin D, 0.09 to 0.5 part of citric acid and 0.09 to 0.45 part of stevioside. The bone peptide composite food is high in nutrient and good in eating effect through matching of various substances.

The invention discloses an application of fructose-1, 6-diphosphate dicalcium salt in a layer feed additive. Layers fed with the feed added with fructose-1, 6-diphosphate dicalcium salt for 45 days are compared with layers fed with the feed without the additive, a result shows that the laying rate is increased by 11.58%, the death rate is decreased by 80%, and the economic benefits of layer feeding are greatly increased.

The invention discloses application of fructose-1, 6-diphosphate in improving the anti-hypoxia ability of the body. The invention discloses application of fructose-1, 6-diphosphate in preparing products for improving the anti-low oxygen or anti-hypoxia ability of animals. Fructose-1, 6-diphosphate serves as a human body metabolic intermediate, exogenous supplementation of fructose-1, 6-diphosphate can improve the body's resistance to plateau hypoxia, and improve the athletic ability and anti-fatigue ability of the body in a plateau hypoxia environment.

The invention provides a high-energy nutrient composition. Active ingredients of the high-energy nutrient composition include, by weight, 500-1000 parts of 1, 6-fructose bisphosphate, 200-320 parts of manyprickle acanthopanax root, 10-16 parts of rhizoma polygonati extract, and 1.2-1.6 part of wolfberry extract. The high-energy nutrient composition can not only provide high energy, but also can enhance oxidation resistance of a human body and is suitable for a user to take during a large amount of exercise, so that aerobic endurance is improved and fatigue recovery is promoted.

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.

The invention discloses a method for preparing fructose 1, 6-diphosphate, which comprises the following steps of: inoculating saccharomycetes into a culture medium, reacting by taking glucose as a substrate, and carrying out solid-liquid separation, clear liquid collection, ultrafiltration, permeate collection, nanofiltration, trapped fluid collection and drying on the obtained reaction liquid to obtain the fructose 1, 6-diphosphate, wherein the culture medium contains aldehyde compounds. When the method is used for producing the FDP, the single-batch catalytic yield is increased from 60g / L to 85g / L.

The invention provides a sports nutritional food formula and a preparation method thereof. Every 30 g of the sports nutritional food comprises the following raw materials of, by weight, 9.5-10.80 g of anhydrous dextrose, 6.4-8.2 g of maltooligosaccharide, 6.9-9.2 g of cane sugar, 3.5-5.5 g of crystalline fructose, 0.2-0.3 g of 1.6-fructose diphosphate trisodium salt, 0.75-0.9 g of L-carnitine, 0.05-0.15 g of edible salt sodium chloride, 1-3 g of grape seed extract, 0.4-0.8 g of tea polyphenol, 0.4-0.6 mg of thiamine hydrochloride, 0.4-0.6 mg of riboflavin, 0.4-0.6 mg of pyridoxine hydrochloride, 0.1-0.2 g of sodium bicarbonate and 0.5-0.7 g of monopotassium phosphate. According to the formula, carbohydrate distribution is reasonable, sodium salt content proportion is scientific, concentration coordination of carbohydrate and sodium salt is achieved, vitamin B content proportion is reasonable, the effect of exciting the cerebral cortex is achieved, and the purpose of refreshing can be achieved; and in addition, the formula also has the effect of shrinking blood vessels, is beneficial to prolonging the exercise time, is also beneficial to reducing the body fat content, and is beneficial to relieving the exercise oxidative stress.

The invention relates to the technical field of functional foods, in particular to a nutritional supplement. The nutritional supplement comprises the following raw materials in parts by weight of 720-900 parts of carbohydrate, 20-80 parts of 1, 6-fructose diphosphate, 15-70 parts of taurine, 12-70 parts of plant polyphenol and 0.1-0.8 part of vitamin B. The ORAC value of the plant polyphenol is not less than 15000 mu mol TE / g, and the content of oligomeric proanthocyanidins is not less than 45%. The nutritional supplement disclosed by the invention can be stably compounded, and is good in water solubility, proper in osmotic pressure and quick to absorb; and the athletic ability of athletes can be improved, and important organs and tissues of bodies can be protected.

The application relates to the field of agricultural biotechnology, in particular to an Escherichia coli engineering bacterium and a fermentation production method of N-acetylglucosamine. The present invention improves the supply of product precursors in mutant Escherichia coli chassis cells by cutting off the shunting of N-acetylglucosamine synthesis precursor 6-fructose phosphate by truncating the glycolysis pathway and the pentose phosphate pathway, and then utilizing the free high-copy The plasmid expresses the key enzymes of the N-acetylglucosamine synthesis pathway in the chassis cells, and the mixed carbon source medium is used to ferment and produce N-acetylglucosamine, and the yield of N-acetylglucosamine reaches 2.8 g / L.

The invention relates to the technical field of nutritional health-care drinks, in particular to an FDP cyclic adenosine monophosphate compound drink composition with a myocardial protection effect. The formula comprises the following raw materials: 8 g of water, 2 g of a red date extracting solution, 0.15 g of 1, 6-fructose diphosphate trisodium salt, 0.1 g of deer myocardial peptide powder, 0.1 g of anhydrous citric acid, 0.004 g of potassium sorbate, 0.00003 g of vitamin B1, 0.00003 g of vitamin B2 and 0.00003 g of vitamin B6. The preparation method comprises the following steps: molecular docking; performing ultrasonic synergism; carrying out steady-state treatment; and mixing. The invention provides the FDP cyclic adenosine monophosphate compound drink composition with the myocardial protection effect, and the efficacy improvement and clinical application value of a compound myocardial protection product are improved.

The invention discloses a method for separating and purifying 1, 6-fructose diphosphate from a 1, 6-fructose diphosphate reaction liquid, which comprises the following steps: adjusting the pH value of the 1, 6-fructose diphosphate reaction liquid to be acidic, performing microfiltration, collecting permeate liquid, performing first electrodialysis desalination to obtain a reaction liquid after first desalination, loading the reaction liquid to strongly basic anion exchange resin, and performing adsorption, impurity washing and elution to obtain the 1, 6-fructose diphosphate. The method comprises the following steps: adding a sodium salt solution into an eluent containing 1, 6-fructose diphosphate, collecting the eluent containing 1, 6-fructose diphosphate, carrying out second electrodialysis desalination to obtain a 1, 6-fructose diphosphate solution, carrying out nanofiltration concentration, collecting a concentrated solution, crystallizing, carrying out suction filtration, washing, and drying to obtain the 1, 6-fructose diphosphate existing in a sodium salt form. The electrodialysis technology is high in desalination efficiency and high in target substance yield; the resin in the chromatographic separation section can be recycled after being regenerated, so that the separation cost is low; the purity of the fructose 1, 6-diphosphate existing in the form of sodium salt obtained by the separation and purification method exceeds 99.0%, and the total yield exceeds 95%.

The invention discloses a preparation method of a healthy feed additive for protecting hepatopancreas of procambarus clarkii, and belongs to the technical field of healthy breeding of shrimps and crabs. The preparation method comprises the following specific preparation processes: weighing microcrystalline cellulose, glucose, glucose-6-phosphoric acid, fructose-6-phosphoric acid, trehalose, glutamine, glutamic acid, glutamide-6-phosphoric acid, glucosamine, taurine, cholesterol and a mixture of trehalose and glucosamine according to mass parts, uniformly mixing, and storing the mixed mixture under a light-closed refrigeration condition. According to the invention, after the prepared healthy feed additive for protecting hepatopancreas of procambarus clarkii is uniformly mixed with commercialized procambarus clarkia feed according to a ratio of 1: 49, the obtained mixture is easy to absorb, the hepatopancreas form and function of the procambarus clarkia can be remarkably protected, the survival rate and weight gain rate of the procambarus clarkia are increased, and the culture benefit is obviously improved.

The invention provides an application of fructose diphosphate in preparation of a medicine for treating affective disorders. Experimental results of the invention show that the fructose diphosphate can improve depression and anxiety states, and can effectively treat the affective disorders, especially has a significant therapeutic effect on epileptic comorbidity affective disorders caused by epilepsy. Therefore, the fructose diphosphate has very good application prospects in the preparation of the medicine for treating the affective disorders, especially the epileptic comorbidity affective disorders.