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45 results about "Fructose phosphate" patented technology

Frozen stock solution for nerve cells and freezing storage method

The invention discloses cell frozen stock solution for freezing and storing nerve cells and a freezing storage method, wherein, the cell frozen stock solution and the freezing storage method are used for freezing and storing the nerve cells, especially primary animal nerve cells that are isolated freshly, such as hippocampus nerve cells. The frozen stock solution is formed by adding basic medium,fetal bovine serum, dimethyl sulfoxide,6-fructose phosphate and flavonoid compounds to the basic medium; 6-fructose phosphate (w / w) achieves the function of improving cell viability (effective cell rate) when the frozen cells are recovered; and in addition, the flavonoid compounds achieve definite oxidation resistance and can prevent free radical from damaging the cells. The cell frozen stock solution and the freezing storage method can improve the survival rate and the cell function of the nerve cells through freezing storage, the survival rate of unfreezing cells can be increased to reach more than 95 percent, the frozen stock solution for the nerve cells can preserve the nerve cells for a long period, the biological activity of the nerve cells can be ensured, and very high utility values can be achieved.
Owner:温州医学院附属第二医院

Culture solution for primary culture of newly born rat hippocampal neuron and preparation method and application thereof

InactiveCN102533654AMetabolic recovery and improvementAvoid damageCulture processNervous system cellsNutritionSingle cell suspension
The invention discloses culture solution for primary culture of newly born rat hippocampal neuron and a method for primarily culturing the rat hippocampal neuron. The culture solution is formed by adding 1,6 diphosphonic acid fructose, fructose and nutrition additive into base culture solution Neurobasalmedium. The method for primarily culturing the rat hippocampal neuron comprises the following steps: 1 preparing hippocampal neuron culture solution; 2 drawing materials and digesting the materials; 3 preparing single cell suspension; and 4 conducting inoculating and cultivating. The method for primarily culturing the rat hippocampal neuron is simple, convenient and capable of obtaining hippocampal neuron with good growth state and high purity by adopting the culture solution and has important application value and economical value.
Owner:温州医学院附属第二医院

Synthesis method of sugar via extracellular enzyme catalysis

The invention discloses a synthesis method of sugar via extracellular enzyme catalysis, comprising steps that: formaldehyde is reacted with 5-ribulose phosphate under the enzyme catalysis to produce 6-fructose phosphate; the 6-fructose phosphate is converted to the sugar under the enzyme catalysis, or connects glycosyl to an amylum chain or a cellulose chain. The formaldehyde can be produced by hydrogenating or electrolyzing carbon dioxide via the enzyme catalysis or chemical catalysis. The synthesis method also can comprise the step of using the 6-fructose phosphate to regenerate the 5-ribulose phosphate, so as to continuously produce the sugar. The synthesis method of the sugar via the extracellular enzyme catalysis uses a simple reactor, and can adopt the energy-fixed carbon dioxide to industrially produce the sugar.
Owner:黄卫东

Application of composition to the preparation of antiepileptic drug

ActiveCN103599115AAddress serious deficienciesImprove antiepileptic effectNervous disorderHydroxy compound active ingredientsFructose phosphateOral mucous membrane
The invention provides the application of a 1,6-fructose diphosphate sodium salt, fructose and / or borneol composition to the preparation of an antiepileptic drug. The composition consists of 1,6-fructose diphosphate sodium salt and fructose, or 1,6-fructose diphosphate sodium salt and borneol combination, or 1,6-fructose diphosphate sodium salt, fructose and borneol. The composition provided by the invention can improve in vivo stability of 1, 6 1,6-fructose diphosphate, in order to maintain high level of 1,6-fructose diphosphate in the brain for a long time and make the 1,6-fructose diphosphate sodium salt exert antiepileptic effect for a long time. The composition provided by the invention particularly employs chewable tablets, orally disintegrating tablets and dispersible tablets as dosage forms for the antiepileptic drug, and the dosage forms can be directly absorbed in oral mucosa, avoid hepatic first-pass effect and further increase the level of 1,6-fructose diphosphate in the brain, thereby further improving the antiepileptic efficacy of the drug.
Owner:ZHEJIANG UNIV

Recombinant escherichia coli and construction method and application thereof

PendingCN112574936AImprove the ability to produce GDP-L-fucoseWeaken the glycolytic processBacteriaMicroorganism based processesEscherichia coliPhosphotransferase Gene
The invention belongs to the technical field of bioengineering, and relates to construction of genetic engineering strains, in particular to recombinant escherichia coli and a construction method andapplication thereof. According to the recombinant escherichia coli, an undecylenyl phosphate glucose phosphotransferase gene wcaJ is knocked out, manB, manC, gmd, wcaG and srlD genes are recombined and expressed, a pntAB gene, an ndK gene and a gsK gene are further recombined and expressed, and a pfkA gene is knocked out, so that a synthesis path of forming fructose 6-phosphate is enhanced and theglycolysis process of 6-fructose phosphate is weakened, the accumulation of fructose 6-phosphate is facilitated, and thus more precursor substances are provided for the synthesis of GDP-L-fucose; inaddition, the regeneration of the cofactors NADPH and GTP is enhanced, so that the capacity of producing GDP-L-fucose by the recombinant escherichia coli strain is further improved.
Owner:HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1

Sports nutrition composition and preparation method thereof

The invention discloses a sports nutritional composition and a preparation method thereof, which relates to the technical field of sports nutrition foods or sports beverages. The sports nutrition composition comprises following raw materials: 1, 6-diphosphate fructose trisodium salt, nicotinamide mononucleotide, guarana sodium extract, couch grass root extract, rhizoma phragmitis extract, soybeanpeptide, wheat oligopeptide, compound amino acid, compound vitamin, carbohydrate, medium-chain triglyceride microcapsule powder, creatine, taurine and compound minerals. The sports nutrition composition provided by the invention not only contains basic nutrients required by sports people, but also improves the athletic ability, prevents or delays the generation of fatigue and improves the exerciseendurance by regulating energy metabolism, perfecting the body's antioxidant system in a sports state, improving the muscle state and the like.
Owner:NANJING SHENGNUO BIO TECH IND

Preparation method of 4-phosphoric acid erythrose

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.
Owner:ANHUI BBCA FERMENTATION TECH ENG RES

Composition containing silybum marianum extract and fructose 1, 6 diphosphate and application of composition in liver protection, sobering and fatigue resistance

The invention discloses a composition. The composition contains a silybum marianum extract and fructose 1, 6 diphosphate or a derivative thereof, or a salt thereof. The silybum marianum extract is ina silybum marianum extract microcapsule form and / or a silybum marianum extract liposome form and / or a silybum marianum extract self-microemulsifying form, and the composition of the silybum marianum extract and FDP has the effects of reducing liver injury, preventing hangover and resisting fatigue, and particularly can quickly take effect to reduce liver injury and quickly sober up.
Owner:南京北极光生物科技有限公司

Sport nutritional probiotic composition and preparation method thereof

The invention relates to a sport nutritional probiotic composition and a preparation method thereof. The sport nutritional probiotic composition comprises the following raw materials in parts by weight: 10 to 30 parts of creatine, 1 to 3 parts of 1,6-fructose diphosphate, 0.5 to 1 part of lactobacillus plantarum TWK10, 10 to 20 parts of isomalto-oligosaccharide, 5 to 15 parts of fructo-oligosaccharide, 10 to 20 parts of xylooligosaccharide, 0.15 to 0.25 part of lactobacillus rhamnosus Lr-G14, 0.17 to 0.26 part of lactobacillus acidophilus LA-G80, 0.2 to 0.3 part of bifidobacterium bifidum BB-G90 and 0.3 to 0.4 part of bifidobacterium lactis BL-G101. According to the composition, the creatine, the fructose 1,6-diphosphate, the lactobacillus plantarum TWK10, prebiotics and probiotics are taken as raw materials to form the sport nutritional probiotic composition, so that the sport performance can be effectively improved, the fatigue can be relieved, the muscle strength can be increased, the intestinal florae can be adjusted, and the gastrointestinal sport stress symptom can be relieved.
Owner:山东探克生物科技股份有限公司

Construction method of escherichia coli synthetic strain capable of efficiently fermenting isobutanol in low-residual-sugar manner

The invention provides a construction method of an escherichia coli synthetic strain capable of efficiently fermenting isobutanol in a low-residual-sugar manner. Escherichia coli MG 1655 is taken as a host, an isobutanol synthesis strain E.coli LA09 is constructed, and the fact that a conversion reaction from intracellular 6-phosphofructose into 1,6-fructose bisphosphate is a key limitation factor of residual sugar conversion is determined with a metabonomics method. Heterogenous artificial ED metabolic pathway upstream and downstream fragments derived from a strain Zymomonas mobilis ZM4 are constructed, ED upstream and downstream pathways are matched and adjusted with an artificial promoter in combination of synthetic biology, and one escherichia coli synthetic strain E.coli ED capable of efficiently fermenting isobutanol in the low-residual-sugar manner is constructed; with the initial glucose concentration being 45 g / L, fermentation is completed within 30 h, the yield of isobutanol reaches 13.67 g / L, and the fermenting residual sugar concentration is 0.87 g / L, thereby proving that the method is practical.
Owner:TIANJIN UNIV

High-energy nutrient composition

The invention provides a high-energy nutrient composition. Active ingredients of the high-energy nutrient composition include, by weight, 500-1000 parts of 1, 6-fructose bisphosphate, 200-320 parts of manyprickle acanthopanax root, 10-16 parts of rhizoma polygonati extract, and 1.2-1.6 part of wolfberry extract. The high-energy nutrient composition can not only provide high energy, but also can enhance oxidation resistance of a human body and is suitable for a user to take during a large amount of exercise, so that aerobic endurance is improved and fatigue recovery is promoted.
Owner:BEIJING COMPETITOR SPORTS SCI & TECH

Recombinant yeast, construction method thereof and application of recombinant yeast in preparing tyrosol and derivatives

ActiveCN111139194AAltered carbon flux distributionOptimizing Metabolic PathwaysFungiGenetically modified cellsHydroxytyrosolTyrosol
The invention relates to a recombinant yeast, a construction method thereof and an application of the recombinant yeast in preparing tyrosol and derivatives. The recombinant yeast is obtained by introducing an exogenous fructose-6-phosphate phosphoketolase expression gene into a modified yeast cell, and the modified yeast cell is a yeast cell with a metabolic pathway of synthesizing tyrosol through 4-erythrose phosphate and phosphoenolpyruvic acid. The invention first discloses the fact that in a process of expressing fructose-6-phosphate phosphoketolase in yeast, fructose-6-phosphoric acid iscatalyzed into erythrose-4-phosphoric acid and acetyl phosphoric acid while 1,6-fructose diphosphate is synthesized, and xylulose-5-phosphoric acid is catalyzed into glyceraldehyde-3-phosphoric acidand acetyl phosphoric acid, so that distribution of the carbon metabolism flow in yeast is changed, synthesis of an important intermediate substance erythrose-4-phosphoric acid in tyrosol biosynthesisis enhanced, the metabolic pathway for synthesizing tyrosol is optimized, and the yield of tyrosol and derivatives such as hydroxytyrosol and the like is improved.
Owner:SHANDONG HENGLU BIOTECH CO LTD

Preparation and application of glutamine-fructose-6-phosphate aminotransferase coding gene and glutamine-fructose-6-phosphate aminotransferase enzyme

The invention discloses a gene sequence of Oryza sativa Japonica Group glutamine-fructose-6-phosphate aminotransferase (OsGFAT) derived from Oryza sativa Japonica Group, and application of the gene sequence. The invention provides a method for preparing the OsGFAT. The method comprises the following steps: cloning the gene of the OsGFAT to an escherichia coli expression vector to obtain an escherichia coli recombinant strain capable of heterologously expressing the OsGFAT, and preparing OsGFAT by heterologously expressing the strain. The invention also provides the application of the OsGFAT. The OsGFAT is used for catalytic synthesis of GlcN-6-P and Glc-6-P by an enzymic method.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Medicament application of 1, 6-fructose bisphosphate sodium salt containing borneol

InactiveCN103599114AGood anti-epileptic effectAct as a flavoring agentNervous disorderHydroxy compound active ingredientsFructose phosphateOrally disintegrating tablet
The invention provides application of 1, 6-fructose bisphosphate sodium salt containing borneol to preparation of antiepileptic drug, wherein the 1,6-fructose bisphosphate sodium salt and borneol are in the proportion of 100:0.01-100:20. Research shows that borneol can enhance the antiepileptic effect of 1, 6-fructose bisphosphate sodium salt; the synergistic effect of borneol is related to increase of fructose bisphosphate sodium salt level in the brain; and combination borneol can significantly improve the antiepileptic therapeutic effect. The medicament is an oral solid preparation selected from a chewing tablet, a dispersible tablet, an orally disintegrating tablet or a common tablet, and is an efficient antiepileptic candidate medicament.
Owner:ZHEJIANG UNIV

Method for constructing the recombinant yeasts for preparation of tyrosol and derivatives and its application

A recombinant yeast is constructed by introducing an expressed gene of exogenous Fructose-6-phosphate phosphoketolase into a modified yeast cell, and the modified yeast cell is a yeast cell with a metabolic pathway for synthesizing tyrosol via Erythrose-4-phosphate and phosphoenolpyruvate. The present invention discloses for the first time that in the process of expressing Fructose-6-phosphate phosphoketolase in a yeast, Fructose-6-phosphate is synthesized into beta-D-Fructose 1,6-bisphosphate and also catalyzed into Erythrose-4-phosphate and Acetyl-phosphate, and Xylulose-5-phosphate is catalyzed into Glyceraldehydes-3-phosphate and Acetyl-phosphate, which change the metabolic flux distribution of carbon in the yeast, enhance the synthesis of Erythrose-4-phosphate as an important intermediate for the biosynthesis of tyrosol, optimize the metabolic pathway for synthesizing tyrosol, and increase the yields of tyrosol and its derivatives such as hydroxytyrosol.
Owner:SHANDONG HENGLU BIOTECH CO LTD

Formula and preparation method of bone peptide composite food

The invention provides a formula and a preparation method of bone peptide composite food, and relates to the technical field of sports food. The formula of the bone peptide composite food comprises the following raw materials in parts by mass: 75 to 100 parts of polydextrose, 68 to 88 parts of bovine collagen peptide powder, 30 to 50 parts of creatine, 30 to 40 parts of milk mineral salt, 0.08 to 0.4 part of tricalcium phosphate, 3 to 8 parts of lindera aggregata leaf powder, 0.1 to 0.8 part of fructo-oligosaccharide, 0.09 to 0.45 part of saussurea involucrata culture powder, 5 to 12 parts of casein phosphopeptide powder, 0.09 to 0.45 part of chitosan oligosaccharide, 0.06 to 0.2 part of magnesium oxide, 0.09 to 0.1 part of 1, 6-fructose diphosphate trisodium salt, 1 to 4 parts of ginseng powder, 2 to 7 parts of rhizoma polygonati powder, 3 to 10 parts of fructus lycii powder, 1 to 8 parts of rhizoma curcumae longae powder, 2 to 6 parts of fructus hippophae powder, 3 to 8 parts of bovine bone peptide powder, 0.08 to 0.12 part of sodium hyaluronate, 0.02 to 0.08 part of vitamin C, 0.04 to 0.06 part of ferric pyrophosphate, 0.02 to 0.05 part of vitamin B6, 0.02 to 0.05 part of vitamin B12, 0.03 to 0.06 part of vitamin D, 0.09 to 0.5 part of citric acid and 0.09 to 0.45 part of stevioside. The bone peptide composite food is high in nutrient and good in eating effect through matching of various substances.
Owner:丛志甲

Application of fructose-1, 6-diphosphate dicalcium salt in layer feed additive

The invention discloses an application of fructose-1, 6-diphosphate dicalcium salt in a layer feed additive. Layers fed with the feed added with fructose-1, 6-diphosphate dicalcium salt for 45 days are compared with layers fed with the feed without the additive, a result shows that the laying rate is increased by 11.58%, the death rate is decreased by 80%, and the economic benefits of layer feeding are greatly increased.
Owner:NANJING BIOTOGETHER

Application of fructose-1, 6-diphosphate in improving anti-hypoxia ability of body

The invention discloses application of fructose-1, 6-diphosphate in improving the anti-hypoxia ability of the body. The invention discloses application of fructose-1, 6-diphosphate in preparing products for improving the anti-low oxygen or anti-hypoxia ability of animals. Fructose-1, 6-diphosphate serves as a human body metabolic intermediate, exogenous supplementation of fructose-1, 6-diphosphate can improve the body's resistance to plateau hypoxia, and improve the athletic ability and anti-fatigue ability of the body in a plateau hypoxia environment.
Owner:INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL

Application of fructose 1,6-diphosphate in preparation of medicine for spontaneous abortion

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.
Owner:THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV

Sports nutritional food formula and preparation method thereof

The invention provides a sports nutritional food formula and a preparation method thereof. Every 30 g of the sports nutritional food comprises the following raw materials of, by weight, 9.5-10.80 g of anhydrous dextrose, 6.4-8.2 g of maltooligosaccharide, 6.9-9.2 g of cane sugar, 3.5-5.5 g of crystalline fructose, 0.2-0.3 g of 1.6-fructose diphosphate trisodium salt, 0.75-0.9 g of L-carnitine, 0.05-0.15 g of edible salt sodium chloride, 1-3 g of grape seed extract, 0.4-0.8 g of tea polyphenol, 0.4-0.6 mg of thiamine hydrochloride, 0.4-0.6 mg of riboflavin, 0.4-0.6 mg of pyridoxine hydrochloride, 0.1-0.2 g of sodium bicarbonate and 0.5-0.7 g of monopotassium phosphate. According to the formula, carbohydrate distribution is reasonable, sodium salt content proportion is scientific, concentration coordination of carbohydrate and sodium salt is achieved, vitamin B content proportion is reasonable, the effect of exciting the cerebral cortex is achieved, and the purpose of refreshing can be achieved; and in addition, the formula also has the effect of shrinking blood vessels, is beneficial to prolonging the exercise time, is also beneficial to reducing the body fat content, and is beneficial to relieving the exercise oxidative stress.
Owner:叶姝 +1

Nutritional supplement

The invention relates to the technical field of functional foods, in particular to a nutritional supplement. The nutritional supplement comprises the following raw materials in parts by weight of 720-900 parts of carbohydrate, 20-80 parts of 1, 6-fructose diphosphate, 15-70 parts of taurine, 12-70 parts of plant polyphenol and 0.1-0.8 part of vitamin B. The ORAC value of the plant polyphenol is not less than 15000 mu mol TE / g, and the content of oligomeric proanthocyanidins is not less than 45%. The nutritional supplement disclosed by the invention can be stably compounded, and is good in water solubility, proper in osmotic pressure and quick to absorb; and the athletic ability of athletes can be improved, and important organs and tissues of bodies can be protected.
Owner:BEIJING HUAJIN PHARM CO LTD

Escherichia coli engineering bacteria and fermentation production method of n-acetylglucosamine

ActiveCN112877272BIncrease precursor supplyIncrease supplyBacteriaHydrolasesBiotechnologyEscherichia coli
The application relates to the field of agricultural biotechnology, in particular to an Escherichia coli engineering bacterium and a fermentation production method of N-acetylglucosamine. The present invention improves the supply of product precursors in mutant Escherichia coli chassis cells by cutting off the shunting of N-acetylglucosamine synthesis precursor 6-fructose phosphate by truncating the glycolysis pathway and the pentose phosphate pathway, and then utilizing the free high-copy The plasmid expresses the key enzymes of the N-acetylglucosamine synthesis pathway in the chassis cells, and the mixed carbon source medium is used to ferment and produce N-acetylglucosamine, and the yield of N-acetylglucosamine reaches 2.8 g / L.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Preparation method of healthy feed additive for protecting hepatopancreas of procambarus clarkii

The invention discloses a preparation method of a healthy feed additive for protecting hepatopancreas of procambarus clarkii, and belongs to the technical field of healthy breeding of shrimps and crabs. The preparation method comprises the following specific preparation processes: weighing microcrystalline cellulose, glucose, glucose-6-phosphoric acid, fructose-6-phosphoric acid, trehalose, glutamine, glutamic acid, glutamide-6-phosphoric acid, glucosamine, taurine, cholesterol and a mixture of trehalose and glucosamine according to mass parts, uniformly mixing, and storing the mixed mixture under a light-closed refrigeration condition. According to the invention, after the prepared healthy feed additive for protecting hepatopancreas of procambarus clarkii is uniformly mixed with commercialized procambarus clarkia feed according to a ratio of 1: 49, the obtained mixture is easy to absorb, the hepatopancreas form and function of the procambarus clarkia can be remarkably protected, the survival rate and weight gain rate of the procambarus clarkia are increased, and the culture benefit is obviously improved.
Owner:YANCHENG INST OF TECH

Application of fructose diphosphate in preparation of medicament for treating affective disorders

The invention provides an application of fructose diphosphate in preparation of a medicine for treating affective disorders. Experimental results of the invention show that the fructose diphosphate can improve depression and anxiety states, and can effectively treat the affective disorders, especially has a significant therapeutic effect on epileptic comorbidity affective disorders caused by epilepsy. Therefore, the fructose diphosphate has very good application prospects in the preparation of the medicine for treating the affective disorders, especially the epileptic comorbidity affective disorders.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV

Treatment agent for laying hen pecking and preparation method thereof

The invention provides a treatment agent for laying hen pecking and a preparation method thereof. Firstly, serratia marcescens ATCC14756 and clostridium butyricum ATCC19398 are respectively activatedand singly fermented, and are then mixed to prepare seed liquid; next, rhizoma acori tatarinowii and spirulina are mixed and extracted to prepare mixed slurry; finally, the mixed slurry is added intothe seed liquid; and mixed fermentation is performed. The serratia marcescens and the clostridium butyricum can produce various metabolites after the fermentation treatment; and antibacterial performance and immune enhancing performance are realized. The rhizoma acori tatarinowii has the sedation effect. The spirulina can be used for regulating various body functions. D-fructose-1,6-diphoshatecalcium salt can provide an energy source on one hand, and can provide a calcium source on the other hand. By using the treatment agent provided by the invention, antibacterial and anti-inflammatory effects can be achieved; the occurrence and diffusion of other complication diseases can be avoided; the sedation effect is achieved on laying hens; the further diffusion of the pecking is primarily controlled; meanwhile, the immunity of the laying hens is improved; the requirements of the laying lens on amino acids, vitamins and mineral substances are met; and the reoccurrence of the laying hen pecking is avoided from the source.
Owner:长沙凯泽工程设计有限公司
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