Preparation and application of glutamine-fructose-6-phosphate aminotransferase coding gene and glutamine-fructose-6-phosphate aminotransferase enzyme

A technology of aminotransferase and fructose phosphate, applied in the direction of transferase, application, genetic engineering, etc., can solve the problem that GFAT has no relevant transformation research
CN112852774APending Publication Date: 2021-05-28DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2021-05-28

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Abstract

The invention discloses a gene sequence of Oryza sativa Japonica Group glutamine-fructose-6-phosphate aminotransferase (OsGFAT) derived from Oryza sativa Japonica Group, and application of the gene sequence. The invention provides a method for preparing the OsGFAT. The method comprises the following steps: cloning the gene of the OsGFAT to an escherichia coli expression vector to obtain an escherichia coli recombinant strain capable of heterologously expressing the OsGFAT, and preparing OsGFAT by heterologously expressing the strain. The invention also provides the application of the OsGFAT. The OsGFAT is used for catalytic synthesis of GlcN-6-P and Glc-6-P by an enzymic method.
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Description

technical field

[0001] The invention relates to a gene sequence of phosphofructose aminotransferase OsGFAT and a preparation method thereof, in particular to the application of the enzyme in the production of glucose-6-phosphate and glucosamine-6-phosphate. The invention also provides the recombinant plasmid and recombinant genetically engineered strain of the phosphofructose aminotransferase, as well as a method for producing and isolating glucose-6-phosphate and glucosamine-6-phosphate by using the enzyme. Background technique

[0002] Acetylglucosamine (GlcNAc, N-acetyl-β-D-glucosamine) is an amino sugar derivative of glucose. In organisms, it is a monosaccharide with important physiological and biochemical functions after glucose. Often in the form of UDP-GlcNAc, it participates in many biological reaction processes including the nervous system and immune system and the structural composition of various glycoconjugates in organisms. In organisms, the main pathway for g...

Claims

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