Henpox virus universal transfer carrier, recombination henpox virus and its preparation method

A fowlpox virus and transfer vector technology, applied in the field of fowlpox virus universal transfer vector, can solve the problems of difficult application of recombinant virus, low expression efficiency, unfavorable recombinant virus screening, etc. expressive effect
CN1955301AInactive Publication Date: 2007-05-02POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
Publication Date
2007-05-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention relates to a universal transfer vector of poxvirus avium, recombinant poxvirus avium and its preparation. Duplicate nonessential region fragment of Poxvirus avium is used to amplificate and clone for pPCI carrier with PCR. Gene cassette of green fluorescent protein(GFP) is constructed with early or late composed promotor of avian molluscum roup virus, and loxP sequence is transferred into its two sides, and then inserting into pPCI together to obtain universal transfer plasmid vector pHBP, which together with Poxvirus avium genome DNA cotransfecting CEF to obtain recombinant virus rFPVGFP.
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Description

technical field

[0001] The universal fowlpox virus transfer carrier of the invention belongs to the high-tech field of biotechnology, and is specially used for the construction of exogenous gene recombinant fowlpox virus. Background technique

[0002] Fowlpox virus is a member of the genus Fowlpoxvirus of the Poxviridae Vertebratepoxvirinae subfamily. Its host range is narrow and it only infects poultry. It presents abortive infection in mammals, but it does not affect the expression of foreign genes and DNA replication. . Therefore, it can not only be used to develop genetically engineered live vector vaccines for poultry, but also can be used as a non-replicating virus vector to develop mammalian genetically engineered live vector drugs for the prevention or treatment of diseases in animals other than poultry and even humans. The protective antigen genes of avian influenza virus, Newcastle disease virus and Marek's disease virus have been successfully expressed in fowl po...

Claims

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